Navigation Links
Transgenic Animals

Blastocysts are placed onto a petri dish in one or more drops of medium. Blastocysts should be positioned close together in one area of the drop. The ES cells are placed in another drop. All drops on the dish are covered with oil. The method of procedure is as follows: The transfer capillary is directed to the focus level and the position is stored as Position 1. Position 2 is defined beside or above this drop.

The drop with the ES cells is placed in focus and several ES cells are aspirated into the transfer capillary by rotating the knob of the CellTram Oil. The transfer capillary, which now contains the cells, is moved up into the overlay medium. The petri dish is moved in order to bring a blastocyst in the other drop into view. One blastocyst is taken with the second TransferMan (the holding capillary) in the Position 1 and moved to the injection position. In this position, the ES cells are injected into the cavity of this blastocyst.

The injected blastocyst is stored in Position 2 by simply pressing the joystick button of the manipulator on the left-hand side.

Both techniques can be easily performed with the Eppendorf Micromanipulator TransferMan, CellTram Air, CellTram Oil and the FemtoJet.

The automation of repetitive tasks while maintaining easy operation allows the user to concentrate on the micromanipulations themselves. This enables rapid work with good results. Replace one TransferMan with an InjectMan and expand the versatility of the system to include
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2. Transgenic Zebrafish by Electroporation
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transgenic Animals

(Date:5/1/2015)... 2015 According to a ... (Equipment, Consumables), by Type (Monoclonal, Polyclonal), by Technology ... Institutes) - Analysis & Global Forecast to 2019", ... Antibody Production Market for the forecast period of ... reach $2.572 Billion by 2019 from $1.425 Billion ...
(Date:4/30/2015)... Blythewood, South Carolina (PRWEB) May 01, 2015 ... steam system services, is extremely proud to announce that ... have been named as a winner of a Queen’s ... business success for the design and manufacture of a ... is the revolutionary alternative to traditional steam flow ...
(Date:4/30/2015)... 2015   Tamir Biotechnology , a leading developer ... its Ebola antiviral therapy program. Clinical ... currently being evaluated and considered by a number of ... During the first quarter of 2015, the company participated ... The Evaluation of Potential Treatments and Vaccines For Ebola ...
(Date:4/30/2015)... 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ), ... it has commenced an underwritten public offering of its ... underwriters a 30-day option to purchase up to an ... offered in the public offering. The offering is subject ... as to whether or when the offering may be ...
Breaking Biology Technology:Antibody Production Market worth $2.572 Billion by 2019 2Antibody Production Market worth $2.572 Billion by 2019 3Antibody Production Market worth $2.572 Billion by 2019 4Spirax Sarco Receives a Queen’s Award for Innovation 2Spirax Sarco Receives a Queen’s Award for Innovation 3Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 2Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 3Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 4BioAmber Inc. Announces Public Offering Of Common Stock 2BioAmber Inc. Announces Public Offering Of Common Stock 3
... 8 Northwest Biotherapeutics, Inc. (OTC Bulletin Board: NWBO; AIM: ... of a putative securities class action lawsuit, In re ... The Company has agreed to pay in settlement US$1 ... The settlement must be approved by the Court. ...
... AcelRx Pharmaceuticals, Inc.,announced today it has hired Dr. ... this new position, Dr. Skowronski will be responsible ... medical input,and review of clinical development strategy. ... Chief Medical Officer. , ...
... CHICAGO, Jan. 8 Allscripts announced today that,Tully-Wihr ... signed a,definitive agreement to resell the web-based Allscripts ... to 100 physicians. , ... Founded in 1906, Tully-Wihr has been building ...
Cached Biology Technology:Northwest Biotherapeutics Settles Class Action Complaint, and SEC Investigation Closed Without Action 2Northwest Biotherapeutics Settles Class Action Complaint, and SEC Investigation Closed Without Action 3Tully-Wihr Company Selects Web-Based Allscripts Electronic Health Record for 100 Northern California Physicians 2Tully-Wihr Company Selects Web-Based Allscripts Electronic Health Record for 100 Northern California Physicians 3Tully-Wihr Company Selects Web-Based Allscripts Electronic Health Record for 100 Northern California Physicians 4Tully-Wihr Company Selects Web-Based Allscripts Electronic Health Record for 100 Northern California Physicians 5
(Date:4/14/2015)... , April 14, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... for the 2015 New York Design Awards under the ... New York City Design Awards are part of a ... over 2,500 ratings from the marketplace, industry and judging ...
(Date:4/8/2015)... N.C. , April 8, 2015  Infinisource, ... solutions and services, and Morpho, a leading supplier ... partnership to produce the market,s most advanced biometrically-enabled ... Infinisource,s iSolved NXG time clock is setting a ... and labor data for the small and mid-size ...
(Date:4/2/2015)... , April 2, 2015 ... touch fingerprint sensor FPC1025 from the distributor World Peace ... commence during Q2 2015 although the major part of ... sensors will be used by smartphone manufacturers in ... MSEK is included in the communicated revenue guidance of ...
Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 110 Million 2
... in space create in humans and animals so-called optical flow ... a forward movement, the objects flow by laterally, objects at ... change at all. At a higher level in the visual ... the visual information, so that animals can differentiate between their ...
... major differences between women with serious depression and healthy ... and emotions. , The study adds further evidence ... the brain -- specifically in the endogenous opioid system ... pain and stress-reduction system. The findings also show significant ...
... Professor Nigel Dimmock at the University of Warwick is ... This has been shown to protect animals against ... the full range of influenza A infections, including H5N1 ... The 'protecting virus' provides instant protection, and completely prevents ...
Cached Biology News:Two nerve cells in direct contact 2Two nerve cells in direct contact 3New brain-chemistry differences found in depressed women 2New brain-chemistry differences found in depressed women 3'Protecting virus' offers instant flu protection and converts flu infections into their own vaccines 2'Protecting virus' offers instant flu protection and converts flu infections into their own vaccines 3
... Fluoroskan Ascent FL is ... and luminometric measurement technologies ... list of advanced features ... Luminoskan Ascent. Fluoroskan Ascent ...
... Thermo Forma's stainless ... a patented adjustable mechanism ... 6 different shaking angles, ... rack placement.This universal rack ...
... brings together two mature, well-understood technologies: ... surfaces. This combination produces a sensitive ... detection of molecular binding events without ... Protein-specific capture molecules are immobilized on ...
... NGGC3' 3'CGGN NNNNCCG5' Inactivation Conditions: Heat inactivation ... treatment followed by ethanol precipitation. Storage Conditions: ... EDTA, 1.0mM DTT, 0.01% BSA, 50% glycerol. ... Store at -20C. Unit Definition: One unit ...
Biology Products: