Navigation Links
Transgenic Animals

Blastocysts are placed onto a petri dish in one or more drops of medium. Blastocysts should be positioned close together in one area of the drop. The ES cells are placed in another drop. All drops on the dish are covered with oil. The method of procedure is as follows: The transfer capillary is directed to the focus level and the position is stored as Position 1. Position 2 is defined beside or above this drop.

The drop with the ES cells is placed in focus and several ES cells are aspirated into the transfer capillary by rotating the knob of the CellTram Oil. The transfer capillary, which now contains the cells, is moved up into the overlay medium. The petri dish is moved in order to bring a blastocyst in the other drop into view. One blastocyst is taken with the second TransferMan (the holding capillary) in the Position 1 and moved to the injection position. In this position, the ES cells are injected into the cavity of this blastocyst.

The injected blastocyst is stored in Position 2 by simply pressing the joystick button of the manipulator on the left-hand side.

Both techniques can be easily performed with the Eppendorf Micromanipulator TransferMan, CellTram Air, CellTram Oil and the FemtoJet.

The automation of repetitive tasks while maintaining easy operation allows the user to concentrate on the micromanipulations themselves. This enables rapid work with good results. Replace one TransferMan with an InjectMan and expand the versatility of the system to include
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2. Transgenic Zebrafish by Electroporation
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transgenic Animals

(Date:1/22/2015)... Eckert & Ziegler Vitalea Science (EZVS), a leading ... pharmaceutical and biotech industry, has announced that Dr. Mark ... of Director of Science & Technology. , “Mark brings ... drug metabolism and bioanalysis to EZVS in both pharma ...
(Date:1/22/2015)... (PRWEB) January 21, 2015 Cambridge Semantics, the ... technology, today announced that 2014 was a record-breaking year across ... capabilities of the Anzo Smart Data Platform and our Smart ... utilize insights from diverse data which led to record growth ...
(Date:1/22/2015)... zFlo Motion , a US leader in ... product from a new partner. STT-Systems has developed ... 15 years, with offerings in the optical-motion capture, 3D scanning, ... (inertial measurement unit), iSen, is opening eyes around the world. ...
(Date:1/22/2015)... NEW BRUNSWICK , Nueva Jersey , 22 ... Award for Biomedical Research abre hoy su llamada ... las personas cuya investigación científica ha hecho, o tiene ... de la salud humana. Las nominaciones se aceptarán hasta ...
Breaking Biology Technology:Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 2Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 3Cambridge Semantics Announces Record Results for 2014 23D Motion Capture Just Got Easy 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
... DURHAM, N.C. Scientists drew fittingly from Roman mythology ... after the god Janus, who is usually depicted as ... years, scientists have been fascinated by the tantalizing possibilities ... display devices, sensors and many other devices. However, realizing ...
... , , , HANGZHOU, China, ... Board: CCEL), one of the largest and most established leaders in stem cell ... Biosciences, Inc. (SEB), a privately-held stem cell and genomics technology company located near ... Zhejiang Pr o vince , People,s Re ...
... , COLLEGEVILLE, Pa., Aug. 11 Wyeth ... it received notice that the action date for the U.S. Food ... License Application (BLA) for Prevnar 13(TM), (Pneumococcal 13-valent ... 2009, to December 30, 2009. In response to an FDA ...
Cached Biology Technology:Capping a two-faced particle gives duke engineers complete control 2Cryo-Cell Announces Strategic Partnership With S-Evans Biosciences, Inc. 2Cryo-Cell Announces Strategic Partnership With S-Evans Biosciences, Inc. 3Cryo-Cell Announces Strategic Partnership With S-Evans Biosciences, Inc. 4Cryo-Cell Announces Strategic Partnership With S-Evans Biosciences, Inc. 5Cryo-Cell Announces Strategic Partnership With S-Evans Biosciences, Inc. 6Wyeth Provides Regulatory Update on Prevnar 13* in the United States 2Wyeth Provides Regulatory Update on Prevnar 13* in the United States 3Wyeth Provides Regulatory Update on Prevnar 13* in the United States 4Wyeth Provides Regulatory Update on Prevnar 13* in the United States 5Wyeth Provides Regulatory Update on Prevnar 13* in the United States 6
(Date:1/22/2015)... 13, 2015 Technology Showcase, Hall E -   EyeLock ... today announced it will showcase its EyeLock ID technology integrated ... Department of Energy,s Oak Ridge National Laboratory (ORNL) ... EyeLock,s iris identity authentication technology is being used to validate ...
(Date:1/22/2015)... and POINT ROBERTS, Washington , January 20, ... leading sectors including technology and tech stocks, releases video from ... master pickpocket and security consultant Apollo Robbins . ... talks about the Wocket™ biometric smart wallet, a product of ...
(Date:1/22/2015)... EyeLock, Inc. , a market leader of iris-based identity authentication ... to the new role of Senior Vice President of Mobile ... mobile platforms and wearable solutions for EyeLock,s technologies. Gerber brings ... semiconductor industry to his role at EyeLock, with expertise in ...
Breaking Biology News(10 mins):EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2
... trying to find ways to get more energy out of the ... and carbon dioxide and then convert it to oxygen and sugars. ... dispersed throughout the plant like that sweet corn we look ... products we use is not as efficient as we would like. ...
... omega-3 fatty acid is about to improve an American ... move biotechnology onto the average consumer,s daily radar. U.S. ... environmental impacts and increase production to feed a growing ... , Recently, the U.S. Food and Drug Administration issued ...
... BEDFORD, Mass. , March 16 ... provider of imaging and biometrics software, today announced ... Martin,s Advanced Technology Workstation (ATW).  Lockheed Martin recently ... ATWs for the Next Generation Identification (NGI) system. ...
Cached Biology News:Frogs, foam and fuel: University of Cincinnati researchers convert solar energy to sugars 2New biotech advance to add heart healthy omega-3s to US diet 2Aware Biometrics Software Provided for Advanced Technology Workstations 2Aware Biometrics Software Provided for Advanced Technology Workstations 3Aware Biometrics Software Provided for Advanced Technology Workstations 4
... a synthetic peptide whose sequence is from the C-terminal end of the ... extends from amino acids 429 to 448 of RAR*. The sequence ... - V - S - P - S - S - V ... - Q - S - P - L - L - Q ...
... is the next step in the evolution ... track system that delivers labware to multiple ... 10 times the throughput of ordinary microplate ... a variety of configurations to meet any ...
... use of small interfering RNA (siRNA) molecules ... interference,(RNAi) pathway have generated tremendous interest in ... for experimental use can be,synthesized in vitro, ... into the target cells in vitro or ...
... Varians unique collision reaction interface (CRI) ... of troublesome spectroscopic interferences. The result ... without the worry of complex multipole ... cells. And, by coupling autotuning of ...
Biology Products: