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Transgenic Animals

Blastocysts are placed onto a petri dish in one or more drops of medium. Blastocysts should be positioned close together in one area of the drop. The ES cells are placed in another drop. All drops on the dish are covered with oil. The method of procedure is as follows: The transfer capillary is directed to the focus level and the position is stored as Position 1. Position 2 is defined beside or above this drop.

The drop with the ES cells is placed in focus and several ES cells are aspirated into the transfer capillary by rotating the knob of the CellTram Oil. The transfer capillary, which now contains the cells, is moved up into the overlay medium. The petri dish is moved in order to bring a blastocyst in the other drop into view. One blastocyst is taken with the second TransferMan (the holding capillary) in the Position 1 and moved to the injection position. In this position, the ES cells are injected into the cavity of this blastocyst.

The injected blastocyst is stored in Position 2 by simply pressing the joystick button of the manipulator on the left-hand side.

Both techniques can be easily performed with the Eppendorf Micromanipulator TransferMan, CellTram Air, CellTram Oil and the FemtoJet.

The automation of repetitive tasks while maintaining easy operation allows the user to concentrate on the micromanipulations themselves. This enables rapid work with good results. Replace one TransferMan with an InjectMan and expand the versatility of the system to include
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Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
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3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
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