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Transgenic Animals

al holding of the blastocysts is performed by using CellTram Air. CellTram Oil is used for transferring the ES cells. As aforementioned, one inverted microscope, in combination with Hoffmann modulation contrast optics or differential interference contrast optics, makes the ES cell transfer quick and simple. The setting of the positions is carried out as shown in Fig. 6:
Fig. 6 TransferMan for Transgenic Animals Transfer of ES cells Right-hand side - injection position: The area where the transfer capillary is kept for injection (focus level, magnification x20 or x40 times) is defined as Position 1. Position 2 is its parking position. This can be outside and above the droplet (in the overlay medium). This capillary holder is connected to CellTram Oil. Left-hand side - holding function: The area where the uninjected blastocysts are placed is set to Position 1. Here one blastocyst can be easily taken by using CellTram Air and VacuTip. This blastocyst is moved via the joystick to a central position to inject the ES cells.

After ES cells have been injected, the capillary is moved to a parking position (can be outside the view). This position is defined as Position 2. Pressing the joystick button moves the capillary back to Position 1. The procedure then restarts.

Preparation of the petri dish:
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