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Transgenic Animals

places the injection material directly into the end of the Femtotip.

Transfer of Embryonic Stem Cells - Injection of ES cells into the cavity of blastocysts using a microscope (inverted), micromanipulator equipment and transfer / holding devices [2].

Embryonic stem cells are derived from the inner cell masses of normal blastocysts (early embryos, mostly mouse embryos). These cells are pluripotent, which means they can develop into any type of tissue. Therefore, removing ES cells from the culture and placing them back into an early embryo (blastocyst) allows them to divide and become part of the embryo. Transfection allows foreign genetic material to be inserted in vitro into these cells. The target of this process is homologous recombination with the chromosome of the cell, i.e. introduction or exchange of DNA at one location with homologous DNA sequences. Following transfection, the descendants of one individual cell (clone) are analyzed using microbiological methods to ascertain whether any mutation is present. Cells of the identified clones which showed the desired reaction are multiplied in vitro and injected into blastocysts.

These injected blastocysts are then implanted into the uterus of pseudo-pregnant females.

Setup of the workstation: As for the injection of DNA, two TransferMan devices are used for controlling the holding and transfer capillaries. The actu
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Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2. Transgenic Zebrafish by Electroporation
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
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