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Transgenic Animals

Fig. 5 ES Cell Transfer Microinjection is carried out as follows:
An embryo is brought into the injection position using the holding capillary. The tip of the injection capillary is aligned with the pronucleus and inserted directly into the pronucleus. Approximately 1 pl DNA solution is injected during every injection process. The injection can be considered successful when the pronucleus increases in size. If the volume of the pronucleus does not increase, the injection is considered unsuccessful. The injection would then occur into cytoplasm, where the injection drop shows different contrast. If the tip comes too close to the endogenous DNA during the injection process, the DNA sticks to the capillary when it is pulled out. If this occurs, the capillary should be discarded. The correct injection volume of the Transjector is set by defining the injection time and the injection volume for all embryos. Femtotips and Microloaders Femtotips, the standard capillaries for microinjection, are ideal for the injection of DNA, RNA, proteins and dyes into living cells. The shapes of the capillary tip ensures high cell viability and minimizes blockages by the injection material. The capillary opening is constant, allowing reproducible injection volumes in the femtoliter range.

Femtotips are available in two shapes for injecting cultured cells or suspended cells. Backfilling with an Eppendorf Microloader easily
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