The DNA may be injected into either of these pronuclei with no difference in results. Usually the injection is into the male pronucleus because it is larger than the female nucleus and also because it is closer to the oocyte surface.
These oocytes are subsequently transferred into the uterus of pseudopregnant
recipient animals and develop to term.
The actual holding of the embryo is carried out using CellTram Air.
Two possibilities are available for injecting DNA:
using the CellTram Air as a simple injection device or using the FemtoJet for reproducible and programmable injections.
Together with an inverted microscope, combined with Hoffmann modulation
contrast optics or
differential interference contrast optics, microinjection can be effected quickly and simply.