Navigation Links
Transgenic Animals

cyte, the male and the female pronuclei are visible as individuals under normal light microscope and not fused into a so-called zygote.

The DNA may be injected into either of these pronuclei with no difference in results. Usually the injection is into the male pronucleus because it is larger than the female nucleus and also because it is closer to the oocyte surface.

These oocytes are subsequently transferred into the uterus of pseudopregnant recipient animals and develop to term.

Setup of the workstation: One TransferMan micromanipulator is used for moving the holding capillary, the other TransferMan is used for moving the injection capillary.

The actual holding of the embryo is carried out using CellTram Air.

Two possibilities are available for injecting DNA:
using the CellTram Air as a simple injection device or using the FemtoJet for reproducible and programmable injections.

Together with an inverted microscope, combined with Hoffmann modulation contrast optics or
differential interference contrast optics, microinjection can be effected quickly and simply.

Preparation of the object slide: One drop of medium is placed on the slide. The embryos to be injected (10 to 20) are placed in one area. The whole droplet is covered with oil. Operating:
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2. Transgenic Zebrafish by Electroporation
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transgenic Animals

(Date:1/22/2015)... January 22, 2015 Crystal Diagnostics (CDx) Xpress ... it has received AOAC-PTM Certifications for the six non-O157 Shiga ... O145; collectively referred to as STEC or the “Big-6”) as ... forming unit (cfu) per 325 g of raw ground beef ...
(Date:1/22/2015)... WI (PRWEB) January 22, 2015 Dr. Greg ... the 12th annual Scripps Natural Supplements Pre-Conference seminar on probiotics ... Conference is an annual continuing education conference for health care ... and included the topic of probiotics in health. Dr. Leyer ...
(Date:1/22/2015)... Diagenode, Inc., a leading global ... and complete solutions for epigenetics research, recently launched ... the need for manual processing. The new ChIPettor ... of histones or transcription factors and a semi-automated ...
(Date:1/22/2015)... PALO ALTO, Calif. , Jan. 22. 2015  Varian Medical ... equipment and software, has been honored for its commitment to ... most sustainable companies. Varian is the highest ranked healthcare equipment ... ranking, announced today during the World Economic Forum at ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Varian Honored Among World's 100 Most Sustainable Corporations 2
... WILMINGTON, Mass., Sept. 22 Xenon Corp., a company,internationally ... of Pulsed,Light systems, might have found the silver bullet ... their fight against disease., It,s called Pulsed light. ... flashed on mushrooms -- increases the vitamin D content ...
... China indicates that genetically modified cotton, altered to express the ... but also reduces pests among other nearby crops that have ... new ideas for controlling pests and maximizing crop yields in ... the journal Science on Friday, 19 September. ...
... Manipulating embryo-derived stem cells before transplanting them may hold ... spinal cord injuries in humans. Research published in BioMed ... lead to cell based therapies for victims of paralysis ... risk of transplant induced pain syndromes. , Dr. Stephen ...
Cached Biology Technology:Massachusetts Company May Have Silver Bullet In Vitamin D Fight Against Disease 2Massachusetts Company May Have Silver Bullet In Vitamin D Fight Against Disease 3GM crops protect neighbors from pests 2Breakthrough in spinal injury treatment 2
(Date:1/22/2015)... NEW YORK , Jan. 22, 2015   EyeLock, Inc. ... announced it has appointed Steve Gerber to the ... Gerber will be responsible for leading development of mobile platforms ... 25 years of success and innovation in the semiconductor industry ...
(Date:12/22/2014)... 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD and ... on the growing mobile commerce market, announces it has retained ... for the 2015 International CES debut of the Wocket™ biometric ... be at the NXT-ID booth January 6th and 7 th ...
(Date:12/19/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ) ... Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology ... http://photos.prnewswire.com/prnh/20130307/600769 Following the acquisition ... reading feature with the iPhone 5S. It is currently ...
Breaking Biology News(10 mins):EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2
... inflammation takes a "back door" approach to evade natural ... Broadening knowledge of how viruses cause infection, a new ... to bypass the body's defenses. , "This study helps ... Jeffrey M. Bergelson, M.D., a pediatric infectious diseases specialist ...
... long known that the social insects in the ... unusual mechanism for sex determination: Unfertilized eggs develop ... the development of an unfertilized egg into an ... , One mystery has been the origin of ...
... and computer scientists at the Universtity of Helsinki, Finland, ... how gene expression is controlled. The results of this ... A genome milestone was reached in 2001 when sequencing ... followed by complete chemical read-outs of DNA sequence for ...
Cached Biology News:Research details how a virus hijacks cell signals to cause infection 2Insects that produce males from unfertilized eggs reveal a surprising cellular feat 2Insects that produce males from unfertilized eggs reveal a surprising cellular feat 3Cracking the genetic code for control of gene expression 2
Anti-NeuroD 6 Immunogen: Synthetic peptide. Available Date: 36708...
HOXC11 Antibody...
HOXC11 Antibody...
Anti-MMP-14, catalytic domain, clone LEM-2/15.8 Immunogen: Synthetic peptide: amino acid sequence 218-233 within the catalytic domain Available Date: 38579...
Biology Products: