Navigation Links
Transgenic Animals

cyte, the male and the female pronuclei are visible as individuals under normal light microscope and not fused into a so-called zygote.

The DNA may be injected into either of these pronuclei with no difference in results. Usually the injection is into the male pronucleus because it is larger than the female nucleus and also because it is closer to the oocyte surface.

These oocytes are subsequently transferred into the uterus of pseudopregnant recipient animals and develop to term.

Setup of the workstation: One TransferMan micromanipulator is used for moving the holding capillary, the other TransferMan is used for moving the injection capillary.

The actual holding of the embryo is carried out using CellTram Air.

Two possibilities are available for injecting DNA:
using the CellTram Air as a simple injection device or using the FemtoJet for reproducible and programmable injections.

Together with an inverted microscope, combined with Hoffmann modulation contrast optics or
differential interference contrast optics, microinjection can be effected quickly and simply.

Preparation of the object slide: One drop of medium is placed on the slide. The embryos to be injected (10 to 20) are placed in one area. The whole droplet is covered with oil. Operating:
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2. Transgenic Zebrafish by Electroporation
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transgenic Animals

(Date:8/22/2014)... 2014 Ophthalmic drug delivery technologies ... challenging endeavors as the anatomy, physiology and biochemistry ... the foreign substances. Ocular drug delivery technologies provide ... and minimal use of needles; also it enables ... provides specific targeting within the ocular globe. On ...
(Date:8/22/2014)... August 22, 2014 Nerium International, ... is in the spotlight again. Nerium will showcase ... its NeriumFirmTM Body Contouring Cream to Hollywood’s elite ... Creations during the 66th Primetime Emmy® Awards. , ... extract, third-party clinical trials show that NeriumAD night ...
(Date:8/21/2014)... group at the Institute for Molecular Medicine Finland ... has developed a novel "man and machine" decision ... diagnostic aid was described in PLOS One ... is based on computer vision algorithms similar to ... visualization of only the diagnostically most relevant areas. ...
(Date:8/21/2014)... , Aug. 21, 2014 Telomere Biosciences, ... their Nutraceutical "TELO-20 for Dogs" with Telomerase ... supplement in the world for dogs. Telomeres are ... chromosome in the body. A wealth of ground-breaking ... in Telomere Science and Aging, including Nobel laureates, ...
Breaking Biology Technology:Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 4Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 2Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 3A novel 'man and machine' decision support system makes malaria diagnostics more effective 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3
... p.m., and do you know where your 16-year-old daughter is? ... homework. You wish. Chances are shes probably taking advantage of ... chat rooms, websites and to instant message her friends, including ... has never met in person. , ,Unfortunately, as most parents ...
... with two research grants coming over the transom almost ... a non-profit subsidiary of the Wisconsin Alumni Research Foundation ... study of human embryonic stem cells. , ,The grant ... Human Embryonic Stem Cell Research come from the National ...
... CHICAGO - Here is a joke my dad told me (actually, he ... guys were walking on a beach and they found a bottle with ... out. "Thanks guys! I was in that bottle for 150 years," the ... for letting me out." , ,So the first guy says: "I ...
Cached Biology Technology:Parental Control - Options Vary for Internet Restrictions 2Parental Control - Options Vary for Internet Restrictions 3Double header for research dollars from Washington 2Women in Midwest IT Have to Fight, Work Harder 2Women in Midwest IT Have to Fight, Work Harder 3
(Date:8/22/2014)... . NAIROBI (22 August ... looming as a major threat to food security across the ... last five years, 1.7 million African farmers in 13 countries ... and helped them double or even triple crop yields. , ... Africa (AGRA) focuses on intensive efforts initiated five years ago ...
(Date:8/22/2014)... and clinical research and technological advances, the fight ... spanning multiple fronts. , Work happening in a ... new and more selective therapies for cancer. Researchers ... enzyme overexpressed in certain cancersand they have tested ... Chemistry professor Christopher Cairo and his team synthesized ...
(Date:8/22/2014)... is a multifunctional enzyme that localizes ... neuroprotective and cholesterol-synthesizing activities. DHCR24 overexpression ... amyloid β deposition. Dr. Xiuli Lu ... China constructed two recombinant adenoviruses (Ad-rSYN1-DHCR24-myc ... specifically in neuronal cells. They also ...
Breaking Biology News(10 mins):Effort to confront Africa's soil health crisis helps millions of farmers triple yields 2Effort to confront Africa's soil health crisis helps millions of farmers triple yields 3Effort to confront Africa's soil health crisis helps millions of farmers triple yields 4New enzyme targets for selective cancer therapies 2New enzyme targets for selective cancer therapies 3
... , July 19, 2013 Research ... of the "Iris Biometrics - Global Strategic Business ... (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , This report analyzes the ... Thousands. The report provides separate comprehensive analytics for the ...
... 2013 Scientists have identified higher levels of a receptor ... that may serve as a new drug target for the ... in EMBO Molecular Medicine , show that elevated levels ... transfection", are associated with a lower likelihood of survival for ...
... take calcium supplements to lower their phosphorous levels may be ... those who take other non-calcium based treatments, according to a ... The study, published today in the Lancet , calls ... phosphate levels in patients with chronic kidney disease. The researchers ...
Cached Biology News:Iris Biometrics - Global Strategic Business Report 2Calcium linked to increased risk of heart disease and death in patients with kidney disease 2
...
...
... kits are designed to rapidly and ... using our patented APA Technology. APAgene ... an extraordinarily competitive price. All necessary ... The Locator Kit can be used ...
Rabbit polyclonal to Yaf9 ( Abpromise for all tested applications)....
Biology Products: