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Transgenic Animals

cyte, the male and the female pronuclei are visible as individuals under normal light microscope and not fused into a so-called zygote.

The DNA may be injected into either of these pronuclei with no difference in results. Usually the injection is into the male pronucleus because it is larger than the female nucleus and also because it is closer to the oocyte surface.

These oocytes are subsequently transferred into the uterus of pseudopregnant recipient animals and develop to term.

Setup of the workstation: One TransferMan micromanipulator is used for moving the holding capillary, the other TransferMan is used for moving the injection capillary.

The actual holding of the embryo is carried out using CellTram Air.

Two possibilities are available for injecting DNA:
using the CellTram Air as a simple injection device or using the FemtoJet for reproducible and programmable injections.

Together with an inverted microscope, combined with Hoffmann modulation contrast optics or
differential interference contrast optics, microinjection can be effected quickly and simply.

Preparation of the object slide: One drop of medium is placed on the slide. The embryos to be injected (10 to 20) are placed in one area. The whole droplet is covered with oil. Operating:
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