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Transformation of Nematodes With the Helios Gene Gun

tions or comparative experiments more meaningful. In applying this technique to various nematodes, it is conveniently possible to use a couple of hundred target worms of C. elegans and proceed in comparatively low precision, while DNA injections have to be done individually and precisely into the gonads. Parasitic worms, however, can only be transfected on an individual basis or in small groups by Biolistic gene transfer. In all experiments designed, reporter gene expression is observed independent from distance of initial gold particle target sites, clearly indicating an DNA drift within the entire bodies. Altogether, this technique is a reproducible and efficient method of producing fertile transgenic nematodes.


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