Navigation Links
Transformation of Nematodes With the Helios Gene Gun

DNA transfer rates of up to 1% were observed using this selection method, which became considerably enriched during following generations after further transfer (F3/F4: 20%; F5/F6 up to 90%), proving their genetic fixation.

Besides marker gene rol-6, we have employed two different reporter genes, a mutant form of GFP (green fluorescent protein), which was originally cloned from the jellyfish Aequorea victoria (Prasher et al., 1992; Cody et al., 1993; Chalfie et al., 1994) with enhanced fluorescence (Siemering et al., 1996), and -galactosidase from Escherichia coli. Expression was visualized by standard fluorescence microscopy (GFP; see Figure 4) or by histochemical staining (lacZ).

In both cases we have observed marker gene expression under transcriptional control of the SV40 early promoter (Figure 3 A and B), or of the C. elegans actin-1 core promoter after Helios gene gun mediated delivery of Au/DNA particles into the nematode. The lacZ expression of constructs containing the actin-1 core promoter of C. elegans (Files et al., 1983; Krause et al., 1989; Stone and Shaw, 1993) in DNA transfected L. sigmodontis will even be analyzed histochemically.


Discussion
The Biolistic DNA-transfection procedure, because of its simple mode of handling, has an important potential in various innovative molecular biology applications, such as DNA vaccination, protein targeting, and basic research applications such as gene regulation studies. The new Biolistic technology should therefore be able to substitute for other applications in this field. Also, the prepared DNA/Au particles can be stored for rather long periods, at least up to 8 months, which makes repeti
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Filamentous Fungi by Microprojectile Bombardment
6. Transformation of Filamentous Fungi by High-Voltage Electroporation
7. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
8. Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
9. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transformation Nematodes With the Helios Gene Gun

(Date:1/15/2014)... Ill. , Jan. 15, 2014  Bill Jacobs Automotive, a ... , is teaming up with the Heartland Blood Center and ... blood drive. The drive takes place Saturday, Jan. 18 at ... - 2 p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  ...
(Date:1/15/2014)... and SAN JOSE, California , January 15, ... developing antibody-drug conjugates for cancer, today announced the appointment of ... Dr Reynolds has over 20 years, development experience gained in ... Seattle Genetics. "I am delighted to welcome Tom ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Two champions ... continued their sponsorship of an annual competition for middle ... and access to, innovative STEM study. The competition presents ... TEAMS: Tests of Engineering Aptitude, Mathematics, and Sciences ...
(Date:1/15/2014)... January 15, 2014 AudioNotch is the ... therapy for the treatment of tinnitus. Patients listen to ... and over a period of weeks to months, their tinnitus ... in two forms: Notched Music and Notched White Noise. Now, ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5
... of Angel Soft website Madison, Wis. Imagine, says ... that you actually want to watch it again and again. ... Soft toilet paper by international tissue and paper manufacturer Georgia-Pacific. ... asks him what he is doing," Ramsey told a crowd ...
... exceeded expectations, reaching $3.6 billion in 2002, according to a new ... 2005. , ,In 2002, the state ranked 20 th ... in academic R&D spending. , ,"You'd be hard-pressed to find ... few years," said David Ward, president of NorthStar Economics ...
... has announced the release of Centricity Physician Office ... for physicians that extends the functionality of Centricity Physician ... , , Centricity Physician Office Mobile ... and schedules and to capture charges from any location. ...
Cached Biology Technology:Consumer "engagement" stressed at e-Business seminar 2Consumer "engagement" stressed at e-Business seminar 3New report says Wisconsin R&D spending exceeding expectations 2
(Date:4/16/2014)... From far away, the top of a leaf looks ... exterior is actually made up of a patchwork of ... in how these cells individually take on their own ... Sampathkumar, and colleagues sought to pinpoint the shape-controlling factors ...
(Date:4/16/2014)... muscular atrophy (SBMA) is a rare inherited neuromuscular disorder characterized ... considered it to be essentially an affliction of primary motor ... that control muscle movement. , But in a new study ... Neuron , a team of scientists at the University of ...
(Date:4/16/2014)... director of the Physical Biosciences Division of Lawrence Berkeley ... recognized as a leading authority on the evolutionary design ... to systems and synthetic biology, has been named one ... Award by U.S. Energy Secretary Ernest Moniz. The E.O. ...
Breaking Biology News(10 mins):For cells, internal stress leads to unique shapes 2Mutant protein in muscle linked to neuromuscular disorder 2Berkeley Lab's Adam Arkin wins 2013 Lawrence Award 2Berkeley Lab's Adam Arkin wins 2013 Lawrence Award 3
... University of Wisconsin School of Medicine and Public Health ... taking part in a novel clinical trial investigating if ... of severe coronary artery disease. , The trial, just ... in the Autologous Cellular Therapy CD34-Chronic Myocardial Ischemia (ACT34-CMI) ...
... but also too much iron is bad for our ... where it can lead to organ failure and even ... Researchers from the Innsbruck Medical University, the University of ... now made a surprising discovery that may lead to ...
... play a pivotal role in the inception of Darwin’s theory ... their significance when he collected them during his voyage on ... predict the impact the vast amount of marine microbial DNA ... J. Craig Venter, Ph.D., and his team ?will have on ...
Cached Biology News:UW launches study testing adult stem cells for heart damage repair 2UW launches study testing adult stem cells for heart damage repair 3Putting an old drug to a new use 2Darwin's famous finches and Venter's marine microbes 2Darwin's famous finches and Venter's marine microbes 3Darwin's famous finches and Venter's marine microbes 4Darwin's famous finches and Venter's marine microbes 5
... Express Cloning Checker Kits provide two ... after transformation without time-consuming plasmid preparation. By ... I, all you need to do is ... bacteria directly from the transformation plates into ...
GLP-1 (HYB 147-12)...
... Immunogen KLH-conjugated, synthetic peptide ... (RRGPHRNPG-PGDDDGQRSRSSS) of mouse p19ARF. ... amino acids 62-75. Clone 5-C3-1. ... evaluated by immunoblot. ...
EDTA, tetrasodium salt, 1 kg. Ethylene diaminetetraacetic acid.Dihydrate. Assay: 99-101% (as is).Risks: 36, 37, 38 ; Safety Precautions: 22, 36, 37. Category: Nucleotides & Enzymes & Biochemicals, Ul...
Biology Products: