Navigation Links
Transformation of Nematodes With the Helios Gene Gun

Peter Jackstadt1, Horst Zahner2 and Gerd Hobom1, 1 Institut fr Mikrobiologie und Molekularbiologie der Justus-Liebig Universitt Gieen, Frankfurter Str. 107, D-35392, Gieen, 2 Institut fr Parasitologie der Justus-Liebig-Universitt Gieen, Rudolf-Buchheim-Str. 2, D-35392 Gieen


Introduction
The Biolistic DNA transfer technique was first established for plant cells and plant tissues (Sandford et al., 1987; Jefferson et al., 1987; Bruce et al., 1989; Armaleo et al., 1990). More recent applications have been DNA transfer experiments aiming at DNA vaccination of animals (genetic immunization; Sundaram et al., 1996), for example a bombardment of mice to generate protective antibodies, instead of the DNA injection used for this purpose in the past.

Here we report on the potential to use this convenient method for transfection of nematodes, not only for the free-living soil nematode Caenorhabditis elegans, the well known model organism in its class, but also for the cotton rat filaria Litomosoides sigmodontis, which serves as model for human pathogenic filariae (Zahner, Hobom and Stirm, 1995), such as Wuchereria bancrofti or Brugia spp. These parasitic species cause a severe lymphatic disease called filariasis (elephantiasis), from which more than 100 million human beings suffer worldwide.


Methods
PREPARATION OF ADULT HERMAPHRODITE C. ELEGANS AND COTTON RAT PARASITE L. SIGMODONTIS FOR MICROPARTICLE BOMBARDMENT
C. elegans is cultivated on NGM agar plates (Brenner, 1974). Wild-type worms (strain N2 var. Bristol) are fed on Escherichia coli OP-50 (exogenous uracil dependent mutant) seeded on those pla
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Filamentous Fungi by Microprojectile Bombardment
6. Transformation of Filamentous Fungi by High-Voltage Electroporation
7. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
8. Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
9. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Transformation Nematodes With the Helios Gene Gun

(Date:6/19/2013)... June 19, 2013 Applied Rigaku Technologies, ... that details the measurement of sulfur in ultra-low sulfur ... high resolution benchtop EDXRF analyzer . The analysis detailed ... method ISO 13032. This International Standard specifies an ... determination of sulfur content in automotive gasoline. , Regulations ...
(Date:6/18/2013)... Santa Clara, CA (PRWEB) June 18, 2013 ... compared to standard size SEMs but have limited performance ... and lower resolution. Full size SEMs would normally provide ... require more knowledge to operate and have a higher ... SEMs address the gap between these two types ...
(Date:6/18/2013)... June 18, 2013 (PRWEB) June 18, 2013 ... and attendance system in North America, has introduced ... employee data and payroll records between ExakTime’s software ... automates ExakTime software setup and keeps employee ... employee data from a business’ current payroll software, ...
(Date:6/18/2013)... Wilmington, DE (PRWEB) June 18, 2013 ... teachers to the 11th annual DuPont National AgriScience Teachers ... Chesapeake Farms in Chestertown, Md. , ... sponsored by DuPont and is a special project of ... National Association of Agricultural Educators (NAAE) . The ...
Breaking Biology Technology:Rigaku Publishes New Application Note for Analysis of ULSD Per ISO 13032 2Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 2Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 3ExakTime Introduces AccountLinx Plus 2DuPont Sponsors National AgriScience Ambassador Academy 2DuPont Sponsors National AgriScience Ambassador Academy 3
... 20 CV Therapeutics, Inc. (Nasdaq: CVTX ... thoroughly reviewed and rejected the previously announced unsolicited proposal ... a price of $16.00 per share. After careful ... legal advisors, the CV Therapeutics board concluded that the ...
... AMSTERDAM, February 20 Elsevier announced today,that ... abstract and,citation (A&C) database, was chosen by ... in Research for Australia (ERA) initiative. , ... will assess research quality within Australia,s higher,education ...
... DUBLIN, February 20 Shire plc,(LSE: SHP, NASDAQ: ... it has signed an agreement with UCB to acquire ... (excluding the,USA, Canada and Barbados) used for the treatment ... EQUASYM XL is approved and marketed in 10 ...
Cached Biology Technology:CV Therapeutics' Board of Directors Rejects Astellas Unsolicited Proposal 2CV Therapeutics' Board of Directors Rejects Astellas Unsolicited Proposal 3CV Therapeutics' Board of Directors Rejects Astellas Unsolicited Proposal 4The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 2The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 3Shire to Enter European ADHD Market With the Acquisition of EQUASYM(R) IR and XL 2Shire to Enter European ADHD Market With the Acquisition of EQUASYM(R) IR and XL 3Shire to Enter European ADHD Market With the Acquisition of EQUASYM(R) IR and XL 4
(Date:6/19/2013)... further proof that the divergence of humans from chimpanzees ... profoundly influenced by mutations to DNA sequences that play ... study, published June 9 in Nature Genetics , ... genes must play an important role in evolution since ... the proteins produced by genes. Indeed, human and chimpanzee ...
(Date:6/18/2013)... University of Calgary,s Faculty of Medicine have discovered a ... harmful bacteria. Platelets, a component of blood typically associated ... bacteria, and upon detection, seal it off from the ... in Nature Immunology this week, provide the ... "The science community has known that platelets do participate ...
(Date:6/18/2013)... Laboratory of Molecular Biology, has been honored as ... Award. Torrent is being recognized for his outstanding ... predict antimicrobial regions in proteins. Luis Rivas, Centro ... this algorithm to interrogate full genomes in order ... appealing results." , Torrent obtained his Ph.D. ...
Breaking Biology News(10 mins):New research backs theory that genetic 'switches' play big role in human evolution 2New research backs theory that genetic 'switches' play big role in human evolution 3Immunity mechanism discovered 2The American Society for Microbiology honors Marc Torrent 2
... engine that could, the University of Nevada, Reno experiment ... along, dwarfed by the million-gallon tanks, pipes and pumps ... the plant,s electrical power could be supplied on-site by ... very pleased with the results of the demonstration testing ...
... from discoveries of ancient tools and artifacts may need ... finding suggested by a new study that for the ... and goats trampling artifacts into mud. In seeking ... new study documented how animal trampling in a water-saturated ...
... BOSTONDana-Farber Cancer Institute,s Belfer Institute of Applied ... and NYSE: SNY) announced today that they have ... to identify and validate novel oncology targets ... novel therapeutics agents directed to such targets and ...
Cached Biology News:University of Nevada, Reno, demonstrates successful sludge-to-power research 2University of Nevada, Reno, demonstrates successful sludge-to-power research 3Taking a new look at old digs: Trampling animals may alter Stone Age sites 2Taking a new look at old digs: Trampling animals may alter Stone Age sites 3Dana-Farber and sanofi-aventis establish cancer research alliance 2
AK6 Antibody...
Reacts with the 80 kDa fragment of the M-chain of human merosin....
Goat anti-mouse gamma chain-HRP...
mu Opioid Receptor splice variant Immunogen: Synthetic peptide corresponding to residues K S C M D R G M R N L L P D D G P R Q E of Mu Opioid Receptor. Storage: -20 C, Avoid Freeze/Thaw Cycle...
Biology Products: