Navigation Links
Transformation of Filamentous Fungi by Microprojectile Bombardment

h addition the suspension was vigorously vortexed and incubated on ice for 10 min. The DNA-coated tungsten particles were then pelleted in a microfuge for 10 seconds. After removing the supernatant, the tungsten particles were washed with ethanol, resuspended in 30 l ethanol, and 8 l loaded onto each of three macrocarrier discs for bombardment in the PDS-1000/He instrument. Target distance (6 cm), vacuum (28 in Hg) and other parameters were identical to conditions optimized for nuclear transformation of S. cerevisiae cells (Heiser, 1992). The bombardment pressure was varied from 400 to 1,600 psi. Bombarded plates were incubated at 37 C for 48 hours. Control plates were bombarded with tungsten particles which were prepared as described above but not coated with plasmid.

Formation and transformation of protoplasts
PEG-mediated transformation of protoplasts was performed as described by Herzog et al ., 1996.

DNA isolations and Southern blot analysis
DNA was isolated from transformed and non-transformed mycelia by the procedure of Raeder and Broda (1985). Samples of DNA were digested with PstI which cuts pRG-1 at a single site (Waring et al ., 1989). Southern blot hybridizations were carried out as outlined by Sambrook et al . (1989). Plasmid DNA probes were radioactively labeled using the Random Primed DNA Labeling Kit from United States Biochemical (Cleveland, Ohio).

Results and Discussion
In initial experiments using M10 tungsten particles, a helium pressure of 1,200 psi, and other bombardment parameters were used as optimized for nuclear transformation of S. cerevisiae (Heiser, 1992). The spore density was varied from 106108 conidia per plate
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by High-Voltage Electroporation
7. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
(Date:7/3/2015)... Houston, Texas (PRWEB) , ... July 03, 2015 ... ... search firm, highly-regarded by Fortune 500 companies and professional service industries alike-- typically ... aimed to make clients laugh, honor a good cause and break from their ...
(Date:7/3/2015)...  Pomerantz LLP announces that a class action lawsuit ... the "Company")(NYSE: PBYI ) and certain of its ... Court, Central District of California , ... class consisting of all persons or entities who purchased ... 2015 inclusive (the "Class Period").  This class action seeks ...
(Date:7/2/2015)... , July 2, 2015  Spherix Incorporated (Nasdaq: ... fostering and monetization of intellectual property, today announced a ... Spherix Incorporated v. Verizon Services Corp. et al. , ... for the Eastern District of Virginia ... 1, 2015 interpreting certain key claims in favor of ...
(Date:7/1/2015)... ... July 01, 2015 , ... It is now possible to separate two ... photon emission computed tomography (SPECT) according to researchers from Amen Clinics, UCLA, Thomas ... brain scans and is believed to be the largest brain imaging study ever, will ...
Breaking Biology Technology:The Alexander Group Declares Their Independence with a Bit of Levity 2SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 2SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 3SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 4Spherix Issues A Litigation Update 2Spherix Issues A Litigation Update 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4
... April 1, 2011 Predictive Biomarker Sciences (PBS-Bio) decodes the ... this week at the 102nd annual conference of the American Association ... Orange County Convention Center in Orlando, Fla. The presentations will ... shows how the drug UNBS1450, made by Unibioscreen, a Belgian firm, ...
... -- Researchers at the Hebrew University of Jerusalem have achieved ... nanocrystal properties with impurity atoms -- a process called doping ... semiconductor nanocrystals. Semiconductor nanocrystals consist of tens to ... width of a human hair. These tiny particles have uses ...
... the first high resolution structure of a nano-scale square made ... a paper in this week,s early online edition of the ... by a team of chemists headed by Thomas Hermann, an ... scientists said the ability to carry structural information encoded in ...
Cached Biology Technology:TGen and PBS-Bio presents 2 abstracts at AACR conference 2'Nanocrystal doping' developed by Hebrew University researchers enhances semiconductor nanocrystals 2
(Date:6/18/2015)... 2015 This report analyzes the worldwide markets ... Product Segments, which includes Immunoassay Analyzers and Reagents: Enzyme ... Immunoassay Systems, and Nephelometric Immunoassay Systems. The report provides ... , Japan , ... Latin America , and Rest of World. ...
(Date:6/17/2015)... HILDEN , Deutschland und GERMANTOWN, Maryland ... Investigator ®   Produktlinie mit ... QIAGEN N.V. (NASDAQ: QGEN ; Frankfurt Prime ... neue STR-Testkits der Produktlinie Investigator ... DNA-Spuren in den Markt eingeführt. Die neuen Kits zur ...
(Date:6/17/2015)... 2015  Synaptics Inc. (NASDAQ: SYNA ), ... that Xiaomi, one of the world,s largest mobile ... ® family of capacitive touchscreen solutions and ... for its latest smartphones, the Xiaomi Mi Note ... in-cell display solutions and DDICs in discrete display ...
Breaking Biology News(10 mins):Global Immunoassay Systems Market 2Global Immunoassay Systems Market 3Global Immunoassay Systems Market 4Global Immunoassay Systems Market 5Global Immunoassay Systems Market 6Global Immunoassay Systems Market 7Global Immunoassay Systems Market 8Global Immunoassay Systems Market 9Global Immunoassay Systems Market 10Global Immunoassay Systems Market 11Global Immunoassay Systems Market 12Global Immunoassay Systems Market 13Global Immunoassay Systems Market 14Global Immunoassay Systems Market 15Global Immunoassay Systems Market 16Global Immunoassay Systems Market 17Global Immunoassay Systems Market 18Global Immunoassay Systems Market 19Global Immunoassay Systems Market 20Global Immunoassay Systems Market 21Global Immunoassay Systems Market 22Global Immunoassay Systems Market 23Global Immunoassay Systems Market 24Global Immunoassay Systems Market 25Global Immunoassay Systems Market 26Global Immunoassay Systems Market 27Global Immunoassay Systems Market 28Global Immunoassay Systems Market 29QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3
... Medical scientists at the University of Alberta have made ... healthy cells while attacking infections. This finding could one ... treatments. , Faculty of Medicine & Dentistry researcher Colin ... journal, Journal of Immunology . His team included ...
... the University of Alberta have made a basic science ... itself. When DNA becomes too damaged it ultimately leads ... Mark Glover and his colleagues published their findings in ... this summer. For years, scientists thought two key proteins ...
... [Brown University] Although preeclampsia occurs in about 3 ... A report of new research, now in press at ... two distinct risk factors combine to affect the odds ... pregnancy complication. The findings suggest there could be new ...
Cached Biology News:UAlberta medical researchers discover how immune system kills healthy cells 2Discovery about DNA repair could lead to improved cancer treatments 2Study details paired risk factors in preeclampsia 2Study details paired risk factors in preeclampsia 3
Applications: Epitope mapping, Mapping protein-protein contacts, Identification of enzyme substrates/inhibitors, Identification of receptor agonists/antagonists...
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Request Info...
Biology Products: