Navigation Links
Transformation of Filamentous Fungi by Microprojectile Bombardment

Roland W. Herzog, Molecular Genetics Program, Department of Botany and Microbiology, 101 Life Sciences Building, Auburn University, AL 36849-5407, USA


Introduction
The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or electroporation. Both methods typically require preparing protoplasts or osmotically sensitive cells prior to transformation (Herzog et al ., 1996; Goldman et al ., 1990; a detailed review of fungal transformation systems is given by Lemke and Peng, 1995). Over the last few years, microprojectile bombardment has become a powerful tool for transformation of intact cells, particularly for the transformation of fungal strains that do not yield sufficient numbers of regenerating protoplasts or species that cannot be grown in culture, such as obligate plant pathogens. Biolistic transformation of Saccharomyces cerevisiae cells was optimized using the PDS-1000/He system (Heiser, 1992). In addition, several filamentous fungi have been transformed by means of microprojectile bombardment (Table 1). In this study, the PDS-1000/He system was used for stable nuclear transformation of an Aspergillus nidulans strain deficient in pyrimidine synthesis by selection for prototrophic transformants. The effect of conidial density, helium pressure, and size of microprojectiles on transformation frequency were investigated. Transformation frequency, mitotic stability of transformants, and Southern blot hybridization patterns were compared between conidia transformed by particle bombardment and protoplasts transformed by chemical (PEG-mediated) transformation. In addition, optimized condition
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by High-Voltage Electroporation
7. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
(Date:6/1/2015)... N.J. , June 1, 2015  Janssen ... the Phase 3 multicenter study SAR3007, which demonstrated ... trabectedin (YONDELIS ® ) compared to dacarbazine in ... previously treated with an anthracycline and at least ... randomized Phase 3 study ever conducted in this ...
(Date:6/1/2015)... -- About Bio-alcohols Bio-alcohols are organic ... Bio-alcohols are primarily used in the transportation sector. For ... gasoline in cars. Technavio,s analysts forecast the global bio-alcohols ... over the period 2014-2019. Covered in ... into five: transportation, infrastructure, medical, and others. Technavio,s report, ...
(Date:6/1/2015)... , June 1, 2015  Novartis is highlighting ... of CTL019, an investigational chimeric antigen receptor (CAR) ... the treatment of specific types of hard-to-treat non-Hodgkin ... the University of Pennsylvania,s Perelman School of Medicine ... diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma ...
(Date:6/1/2015)... , June 1, 2015  The Val Skinner ... national innovative science curriculum for high school students focused ... 16 th annual LIFE Event (LPGA Pros in ... Ridge Country Club in West Caldwell, N.J. ... LPGA,s biggest stars raised more than $500,000 on Monday, ...
Breaking Biology Technology:Trabectedin Improves Disease Control in Patients with Advanced Soft Tissue Sarcoma 2Trabectedin Improves Disease Control in Patients with Advanced Soft Tissue Sarcoma 3Trabectedin Improves Disease Control in Patients with Advanced Soft Tissue Sarcoma 4Trabectedin Improves Disease Control in Patients with Advanced Soft Tissue Sarcoma 5Trabectedin Improves Disease Control in Patients with Advanced Soft Tissue Sarcoma 6Global Bio-alcohols Market 2015-2019 2Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 2Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 3Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 4Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 5Novartis highlights CTL019 data showing its potential in the treatment of specific types of hard-to-treat non-Hodgkin lymphoma 6Val Skinner Foundation Announces Partnership With Discovery Education 2
... Amicus Therapeutics (Nasdaq: FOLD ) will announce ... of markets on Thursday, May 7, 2009. The results ... and webcast that afternoon at 5 p.m. EDT. Interested ... 5 p.m. EDT by dialing 877-874-1565 (U.S./Canada) or 719-325-4830 ...
... Microfluidics and Imaging -- Net Loss Narrowed by $0.07 per ... (Nasdaq: CALP ) today reported its first quarter ... million compared to $29.3 million in the first quarter of ... 2008 included $2.9 million of revenue related to product lines ...
... of its fifth Best Practices Awards program. Grand Prize winners ... Broad Institute of MIT and Harvard, Genedata, Genentech, GlaxoSmithKline, Merck & ... Research. , ... Needham, Mass (PRWEB) April 29, 2009 ...
Cached Biology Technology:Amicus Therapeutics Announces First Quarter 2009 Financial Results Release Date 2Caliper Life Sciences Reports First Quarter 2009 Results 2Caliper Life Sciences Reports First Quarter 2009 Results 3Caliper Life Sciences Reports First Quarter 2009 Results 4Caliper Life Sciences Reports First Quarter 2009 Results 5Caliper Life Sciences Reports First Quarter 2009 Results 6Caliper Life Sciences Reports First Quarter 2009 Results 7Caliper Life Sciences Reports First Quarter 2009 Results 8Caliper Life Sciences Reports First Quarter 2009 Results 9Caliper Life Sciences Reports First Quarter 2009 Results 10Bio-IT World Announces the Winners of its 2009 Best Practices Awards Program 2Bio-IT World Announces the Winners of its 2009 Best Practices Awards Program 3Bio-IT World Announces the Winners of its 2009 Best Practices Awards Program 4Bio-IT World Announces the Winners of its 2009 Best Practices Awards Program 5Bio-IT World Announces the Winners of its 2009 Best Practices Awards Program 6
(Date:5/11/2015)... May 11, 2015  Through a well-rounded UAS delegation representing ... had a strong showing at AUVSI,s Unmanned 2015 conference last ... Ohio,s UAS industry met with over ... all points along the UAS ecosystem. "Our ... Vice President for Aerospace Rich Knoll . "If you ...
(Date:5/8/2015)... -- Synaptics Inc. (NASDAQ: SYNA ), the leading developer ... executive management team will present at the following investor events: ... Conference Date: May 18, 2015 Time: 10:40am ET ... Cowen and Company Technology, Media & Telecom ... New York Palace Hotel, New York, NY ...
(Date:5/5/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... mobile commerce market, reminds investors and media that  Mr. ...  at CARTES SECURE CONNEXIONS AMERICA 2015, held in ... The three-day conference is organized into a series of nine ... Global Fraud: Where is the Trust in Cyberspace? ...
Breaking Biology News(10 mins):Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2Synaptics to Present at Upcoming Investor Conferences 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3
... Meeting on Acoustics -- a major international acoustics conference ... Coral Beach Hotel in Cancun, Mexico. Reporters are invited ... http://www.acoustics.org/press The press room contains news releases ... more than a thousand presentations at the meeting, which ...
... With so few Americans consuming the recommended amounts of fruit ... fruit servings and the important nutrients they provide ... of 100 percent juice supplies one serving (1/2 cup) of ... New research presented today at the American Dietetic Association (ADA) ...
... UNC scientists describes how cells infected by the Epstein-Barr ... changing their cellular "cargo" of proteins and RNA. This ... of recipient cells from benign to cancer-producing. In ... potentially manipulate other cells throughout the body. The findings ...
Cached Biology News:Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19 2Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19 3Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19 4Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19 5Drinking 100 percent fruit juice is linked to higher intake of essential nutrients 2UNC scientists identify cellular communicators for cancer virus 2
Schneider's Drosophila - Insect medium...
... more than doubles the benefits of high ... analysis cycles of the standard binary system ... tandem mode. Difficult separations are dramatically improved ... heartcut configurations, on-line sample preparation is fully ...
... the gene supplied by a customer. Mutated DNA ... choice to obtain a library of the mutated ... a desired value within 1-20 mutations per kb. ... be restricted to a desired region of a ...
... Highly-purified, endotoxin-free plasmid DNA preparations are ... in quantities from 1mg to multi-gram ... to customer needs, and glycerol stocks ... , Transformation, clonal selection (Clonal selection ...
Biology Products: