Navigation Links
Transformation of Filamentous Fungi by High-Voltage Electroporation

400 l produced lower numbers of transformants. This result could be expected since reducing the cross-sectional area of the solution at the electrode surface increases the resistance (Shigekawa and Dower, 1988).


Conclusions
There are two major advantages of electroporation over the traditional chemical method of transformation. The first is simplicity: the OSC do not need to be purified by sorbitol gradients, and it is possible to perform many electro-transformations at one time. The second advantage we found is that electroporation is more reproducible than PEG-mediated transformation. An important difference we observed between the two methods was the stability of transformants from T. harzianum when transformed with the plasmid pAN7-1. A possible explanation for this effect could be the activation of the repair system caused by the high-voltage electric pulse.

The results presented here show that OSC from T. harzianum can be efficiently transformed by electroporation. Electroporation is rapid, easy to perform, and requires minimal sample preparation. Therefore, it may prove to be a general method useful for introducing DNA into many fungal species in addition to T. harzianum, A. nidulans (Richey et al., 1989), F. solani (Richey et al., 1989), S. cerevisiae (Delorme, 1989; Hashimoto et al., 1985; Hill, 1989; Karube et al., 1985; Meilhoc et al., 1990; Rech et al., 1990; Weaver et al., 1988), S. pombe (Hood and Stachow, 1990; Weaver et al., 1988), and D. discoideum (Dynes and Firtel, 1989; Egelhoff et al., 1989; Howard et al., 1988). This report presents possibilities to improve transformation systems that have already been described, or to transform other filamentous fungi where
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by Microprojectile Bombardment
7. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
10. Optimizing Chemical Transfection and Electroporation of siRNAs
11. High Throughput siRNA Electroporation
Post Your Comments:
(Date:6/18/2013)... Angeles, CA (PRWEB) June 18, 2013 (PRWEB) June ... construction’s #1 time and attendance system in North ... software that shares employee data and payroll records ... AccountLinx Plus automates ExakTime software setup ... The software imports employee data from a business’ ...
(Date:6/18/2013)... The human skin is the largest organ, ... human body part. Similar to the liver, or heart, the ... repair and grow. Recent reports from the mankatofreepress.com explained how ... as important as other life supporting organs. The article suggests ... cell reproduction, increase the appearance of skin, and fight off ...
(Date:6/18/2013)... 2013 RegeneCure announced today ... AMCA Guided Bone Regeneration (GBR) Dental Membrane as a bone ... common problem encountered when patients have a tooth replaced with ... to house the implant. Consequently there is often a need ... natural bone regenerates. The bone substitute material, in turn, needs ...
(Date:6/18/2013)... , June 18, 2013 ... international investment research firm, announces the initiation ... a French biopharmaceutical company developing and marketing ... care indications.      (Logo: http://photos.prnewswire.com/prnh/20130417/608168) ... comprehensive report examining the investment merits of ...
Breaking Biology Technology:ExakTime Introduces AccountLinx Plus 2Natural Acne Remedies Through Diet, Probiotic Action Shares New Insight on What Foods May Help Lead to Clear Skin 2RegeneCure Starts Clinical Study Using Polymeric Bone Stimulating Membrane for Dental Implants 2RegeneCure Starts Clinical Study Using Polymeric Bone Stimulating Membrane for Dental Implants 3Edison Expands French Healthcare Sector Coverage With Initiation of Coverage on BioAlliance Pharma 2Edison Expands French Healthcare Sector Coverage With Initiation of Coverage on BioAlliance Pharma 3
... Aug. 11, 2011 SimPL, the Simple Platform for ... delivering up-to-date regulatory training , eLearning ... Healthcare administrators and educators spend a great ... SimPL is for those organizations that want a system ...
... Cord Blood America, Inc . ( www.cordblood-america.com ) (OTC Bulletin ... stem cell preservation company focused on bringing the life ... today announced revenues of $2.89 million for the first six ... $1.79 million in the same period in 2010.  Revenues for ...
... Pharma IQ is pioneering new compound management discussion ... Compound Management and Integrity Online event, which will take ... 5 weeks, beginning 31 st   of ... process is high on the agenda for many organisations and this ...
Cached Biology Technology:The SimPL Learning Management System Supports Compliance in Healthcare Facilities 2Cord Blood America Six Month Revenues Up 61 Percent; Gross Profit Up 100 Percent 2Cord Blood America Six Month Revenues Up 61 Percent; Gross Profit Up 100 Percent 3Compound Management and Integrity Goes Online 2Compound Management and Integrity Goes Online 3
(Date:6/17/2013)... alcohol produced by fungi, bacteria, and algae, is a ... is also used in the medical field it,s ... out excess fluids and used during surgery as a ... passage of other drugs. , Now Profs. Ehud Gazit ... Molecular Microbiology and Biotechnology and the Sagol School of ...
(Date:6/17/2013)... gas, is widespread across Pennsylvania, covering up to 280,000 ... Annals of the New York Academy of Sciences ... from toxic chemicals, the building of well pads and ... engendered a great deal of controversy, largely because of ... diversity and resources have scarcely been addressed in the ...
(Date:6/16/2013)... million years ago triggered a similar marine ecosystem crisis ... of global warming, according to research published today in ... from the universities of Newcastle, UK, Cologne, Frankfurt and ... crash in the marine ecosystem during the mid-Cretaceous greenhouse ... time the amplitude and duration of the temperature change.,Analysing ...
Breaking Biology News(10 mins):Artificial sweetener a potential treatment for Parkinson's disease 2Global cooling as significant as global warming 2
... travel ... documents, DALLAS, Dec. 19 ... -- also known as ePassports --,it also recognized there was an opportunity ... Entrust, Inc. (Nasdaq:,ENTU) to provide the public key infrastructure (PKI)-enabled digital,signatures., ...
... the Montreal Neurological Institute of McGill University and the ... Music and Sound Research (BRAMS), has found that tone-deaf ... regions of the brain related to processing musical pitch, ... auditory cortex, as compared to those who are musically ...
... -- Two deadly and highly infectious viruses -- both potential ... new drug developed by scientists at Weill Cornell Medical College ... related, newly recognized zoonotic viruses that can spread from their ... pigs, horses and humans. The mode of transmission ...
Cached Biology News:Entrust Digital Signatures Help Secure More Than 18 Million U.S. ePassports 2Entrust Digital Signatures Help Secure More Than 18 Million U.S. ePassports 3Entrust Digital Signatures Help Secure More Than 18 Million U.S. ePassports 4Jingle bells not merry for tone-deaf individuals 2Jingle bells not merry for tone-deaf individuals 3Drug aimed at 2 bioterror agents blocks live viral infection, Weill Cornell team reports 2Drug aimed at 2 bioterror agents blocks live viral infection, Weill Cornell team reports 3
Molecular Biology grade. Ideally suited for DNA and RNA separation and recovery. High gel strength. Gelling temp. 36C. Melting temp. 88C. EEO: 0.05-0.13 Resolution 0.2-40 kb; Rnase/Dnase: none detec...
Component in MasterPure™ Purification Kits...
... (X/R) Red System consists of concentrated ... and X/R Red Buffer. The X/R ... to produce red staining that may ... , Buffer, 100 mL , Chromogen, ...
... Signets FAST RED Chromogen System ... and substrate buffer. FAST RED ... alkaline phosphatase to produce red ... The FAST ...
Biology Products: