Navigation Links
Transformation of Filamentous Fungi by High-Voltage Electroporation


Electroporation of germinative tubes or mycelia from T. harzianum did not yield transformants, so osmotically sensitive cells were tested. The effect of incubation time of the germinative tubes with the cell wall-degrading enzymes on the electro-transformation efficiency was tested. Intervals of 15, 30, and 45 minutes were used. An incubation period shorter than 30 minutes did not yield any transformants while a 45 minute incubation yielded 50% less transformants than did a 30 minute incubation time (data not shown). To optimize conditions, different combinations of voltages and capacitances were chosen, while holding the parallel resistor at 200 ohms and holding the concentration of OSC at 2.0 x 108 per ml (data not shown). The electroporation medium consisted of distilled water with 1.2 M sorbitol as an osmotic protectant. These preliminary results indicated that transformation could be obtained at 2.0 kV/cm with a capacitance of 25 F. Figure 1A shows that this was a good approximation since the maximum yield of transformants was found at 2.8 kV/cm.

The effect of the parallel resistor (and thus the time constant, τ=RxC) on electroporation efficiency was also examined (Figure 1B). The best yield was obtained when the pulse was delivered using a field strength of 2.0 kV/cm, a parallel resistance of 800 ohms and a capacitance of 25 F. Using 2.0 kV/cm with 25 F, the survival of the OSC decreased sharply at 100 ohms reaching 20% survival, and continued to decrease slowly from 200 ohms to 800 ohms. At 800 ohms, the survival was about 10.0%. From these initial experiments, the best electrical conditions found for the electroporation of T. harzianum were a field strength of 2.8 k
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by Microprojectile Bombardment
7. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
10. Optimizing Chemical Transfection and Electroporation of siRNAs
11. High Throughput siRNA Electroporation
Post Your Comments:
(Date:8/27/2014)... College, PA (PRWEB) August 27, 2014 ... Simulation Software with new features for the biomedical ... is an enhanced Biological Thermal Sensor, exclusive to the ... a specialty version of XFdtd, developed to calculate the ... export functionality has been added to all versions to ...
(Date:8/26/2014)... 26, 2014  NeuroSigma, Inc., a Los ... bioelectronic technologies, today announced that it has filed a ... and Exchange Commission relating to a proposed initial public ... to be offered and the price range for the ... Jefferies LLC will act as the book-running manager for ...
(Date:8/26/2014)... engineers from Singapore has successfully piloted the world,s ... communications. , With the weight of payloads being ... lightweight, low power-consuming, wireless communication system to do ... been a challenge for system designers. , The ... Nanyang Technological University in Singapore launched VELOX-I, which ...
(Date:8/26/2014)... Ga. , Aug. 26, 2014  ViveBio, ... biological sample collection, stabilization and transportation, announced today ... Laboratory Procurement Services, LLC (LPS) of ... of products in the United States ... new class of ambient temperature dried biological specimen ...
Breaking Biology Technology:Remcom Announces Enhanced Biological Thermal Sensor and Other Biomedical Features in XFdtd EM Simulation Software Update 2Remcom Announces Enhanced Biological Thermal Sensor and Other Biomedical Features in XFdtd EM Simulation Software Update 3ZigBee in the Sky 2ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2
... /Xinhua-PRNewswire-FirstCall/ -- American Oriental,Bioengineering, Inc. (NYSE: AOB ... quarter 2008 financial results on Monday, November 10, ... hold a conference call at 8:00 am ET ... 2008 results. Listeners may access the,call by dialing ...
... 30 /PRNewswire-FirstCall/,-- Oridion Systems Ltd. (SWX: ORIDN) ... Foundation Health Plan, Inc., America,s largest,non-profit health ... in the world. The agreement is for ... sampling consumables (FilterLine(R))., With this agreement, ...
... ... Guidance of $0.90 - $1.10 for 2009 and $1.50 - $1.70 ... for 2010, PITTSBURGH, Oct. 30 ... months ended,Sept. 30, 2008., Financial Highlights, -- Adjusted diluted EPS of $0.23 and $0.52 ...
Cached Biology Technology:Oridion Signs Agreement With Major US Health Plan Provider for Microstream(R) Capnography 2Oridion Signs Agreement With Major US Health Plan Provider for Microstream(R) Capnography 3Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 2Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 3Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 4Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 5Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 6Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 7Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 8Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 9Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 10Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 11Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 12Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 13Mylan Reports Adjusted Diluted EPS of $0.23 for the Quarter Ended Sept. 30, 2008 14
(Date:8/27/2014)... of consumers are willing to pay a premium for ... they,re getting what the label claims is another matter. ... a new way to make sure farms are labelling ... Journal of Agricultural and Food Chemistry , could ... Bavarian Health and Food Safety Authority and the Wuerzburg ...
(Date:8/27/2014)... studies have shown that excessive iron is ... disease. Previous studies from Chunyan Guo and ... have shown that baicalin prevented iron accumulation ... transporter 1 expression, and increased ferroportin 1 ... Parkinson,s disease rats. However, the relationship between ...
(Date:8/27/2014)... are greedy bird-brains, University of Warwick research finds ... they make risky decisions, new research has shown. , ... of Warwick,s Department of Psychology, conducted tests that found ... likely to gamble for high-value than low-value rewards. , ... that the test results show the important role that ...
Breaking Biology News(10 mins):Gamblers are greedy bird-brains, University of Warwick research finds 2
... and the U.S.A., the scientists have shown how cutting-edge ... of active proteins in cell organelles at a particular ... use proteins. The work is published in the journal ... cities. They contain all the necessary parts that allow ...
... the use of liquid crystalline polymers (LCP) as a ... sought-after lab-on-a-chip technology. , University of Alberta researchers, collaborating ... Phillips Research Laboratories in the Netherlands, have shown that ... glass backing, can be fabricated and patterned on a ...
... of dengue fever wax and wane over a period ... why. With the incidence and range of the potentially ... these epidemics has never been more important. , A ... suggests that a brief period of cross-immunity conferred by ...
Cached Biology News:A catalogue of proteins 2Polymers show promise for lab-on-a-chip technology 2UGA study explains peaks and troughs of dengue epidemics 2UGA study explains peaks and troughs of dengue epidemics 3
Request Info...
Request Info...
Detect and measure levels of mouse IgG2b antibody isotypes quickly and easily....
Detect and measure levels of mouse IgG3 antibody isotypes quickly and easily....
Biology Products: