Navigation Links
Transformation of Filamentous Fungi by High-Voltage Electroporation


Electroporation of germinative tubes or mycelia from T. harzianum did not yield transformants, so osmotically sensitive cells were tested. The effect of incubation time of the germinative tubes with the cell wall-degrading enzymes on the electro-transformation efficiency was tested. Intervals of 15, 30, and 45 minutes were used. An incubation period shorter than 30 minutes did not yield any transformants while a 45 minute incubation yielded 50% less transformants than did a 30 minute incubation time (data not shown). To optimize conditions, different combinations of voltages and capacitances were chosen, while holding the parallel resistor at 200 ohms and holding the concentration of OSC at 2.0 x 108 per ml (data not shown). The electroporation medium consisted of distilled water with 1.2 M sorbitol as an osmotic protectant. These preliminary results indicated that transformation could be obtained at 2.0 kV/cm with a capacitance of 25 F. Figure 1A shows that this was a good approximation since the maximum yield of transformants was found at 2.8 kV/cm.

The effect of the parallel resistor (and thus the time constant, τ=RxC) on electroporation efficiency was also examined (Figure 1B). The best yield was obtained when the pulse was delivered using a field strength of 2.0 kV/cm, a parallel resistance of 800 ohms and a capacitance of 25 F. Using 2.0 kV/cm with 25 F, the survival of the OSC decreased sharply at 100 ohms reaching 20% survival, and continued to decrease slowly from 200 ohms to 800 ohms. At 800 ohms, the survival was about 10.0%. From these initial experiments, the best electrical conditions found for the electroporation of T. harzianum were a field strength of 2.8 k
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by Microprojectile Bombardment
7. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
10. Optimizing Chemical Transfection and Electroporation of siRNAs
11. High Throughput siRNA Electroporation
Post Your Comments:
(Date:9/17/2014)... RXi Pharmaceuticals Corporation (NASDAQ: ... developing and commercializing innovative therapies addressing major unmet ... has been granted a patent by the United ... delivery system for delivering its "original" RNAi compounds ... treatment of diseases with an inflammatory component. Potential ...
(Date:9/17/2014)... Beckman Coulter , a global leader in ... its agreement with Personify for an additional ... will continue to streamline the Beckman Coulter talent acquisition ... time-to-fill, time-to-source and a deep reduction to cost-per-hire. , ... based on the success of our partnership over the ...
(Date:9/17/2014)... 2014 First antibacterial ... a truly novel class of anti-infective in ... a biopharmaceutical company developing a truly novel ... has secured £4.0m ($6.4m) to advance the ... a range of Gram-positive infections including  Clostridium ...
(Date:9/17/2014)... 2014 The Pistoia Alliance, a ... the barriers to innovation in life sciences R&D ... Carmen Nitsche as Executive Director Business Development North ... global reach. , Carmen joins the Pistoia Alliance ... Development. At Accelrys, she managed various life sciences ...
Breaking Biology Technology:RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 2RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 3RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 4Beckman Coulter Signs 3-year RPO Contract with Personify 2Beckman Coulter Signs 3-year RPO Contract with Personify 3MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 2MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 3MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 4MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 5MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 6MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 7Pistoia Alliance Expands Business Development with Appointment of Carmen Nitsche 2
(Date:9/17/2014)... After decades of decline, grasses have returned to ... eye, the marsh in those places seems healthy again, ... service of the marsh coastal protection remains ... ecosystem function hasn,t come back," said ecologist Mark Bertness, ... the study in the journal Biological Conservation . ...
(Date:9/17/2014)... significantly faster since the 1960s. The typical development phases ... have accelerated -- by as much as 70 percent. ... by scientists from Technische Universitt Mnchen based on long-term ... observed since 1870. Their findings were published recently in ... dieback" was a hot topic, with the very survival ...
(Date:9/17/2014)... crime unit designed to fight food fraud in ... being passed off as beef scientists from Germany ... describe their approach, which represents a vast improvement over ... Food Chemistry . , Hans-Ulrich Humpf and colleagues note ... But they also say that adding, for example, horse ...
Breaking Biology News(10 mins):Cape Cod saltmarsh recovery looks good, falls short 2Cape Cod saltmarsh recovery looks good, falls short 3Global change: Trees continue to grow at a faster rate 2Global change: Trees continue to grow at a faster rate 3
... Biotechnology and Biological Sciences Research Council (BBSRC) Sustainable Bioenergy ... could help us breed grasses with improved properties for ... by a team from the University of Cambridge and ... findings are published today (Tuesday 17 Jan) in the ...
... Nitrogen is both an essential nutrient and a pollutant, ... that feeds billions, a benefit and a hazard, depending ... have spread nitrogen liberally around the planet, say sixteen ... in Ecology series, "Excess Nitrogen in the U.S. ...
... Worldwide pandemics of influenza caused widespread death and illness ... weather patterns around the time of these pandemics finds that ... the equatorial Pacific. The study,s authors--Jeffrey Shaman of Columbia University,s ... Harvard School of Public Healthnote that the La Nia pattern ...
Cached Biology News:Breeding better grasses for food and fuel 2Breeding better grasses for food and fuel 3Solutions for a nitrogen-soaked world 2Does the La Niña weather pattern lead to flu pandemics? 2
Mouse monoclonal [SPM217] to Secretory Component Glycoprotein ( Abpromise for all tested applications)....
Mouse monoclonal [3G8] to MDMA ( Abpromise for all tested applications)....
Shh Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Anti-Cohesin (Scc1/Rad21) Monoclonal Antibody Research Focus: signal transduction Storage: -20C Shipping Temperature: +4C...
Biology Products: