Navigation Links
Transformation of Filamentous Fungi by High-Voltage Electroporation

Contributed by G.H. Goldman, R. Geremia, M. Van Montagu, and A. Herrera-Estrella, Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium


Introduction
Virtually all fungal transformation protocols call for the addition of a high concentration of polyethylene glycol (PEG) following the initial period of exposure to DNA. However, electroporation could also be useful for introducing genes into filamentous fungi. Recently, electroporation has become a valuable technique for transferring nucleic acids into both eukaryotic and prokaryotic cells (Miller et al., 1988; Fster and Neumann, 1989). In this report, we show the use of high voltage-mediated transformation as an efficient method for genetic transformation of Trichoderma harzianum with plasmid DNA.


Materials and Methods
Strains and plasmid. T. harzianum strain IMI206040 was used as the transformation host. The plasmid used was pAN7-1, a derivative of pUC19 containing the E. coli hygromycin B phosphotransferase gene as a dominant selectable marker, and the gpd promoter and trpC terminator signals from Aspergillus nidulans (Punt et al., 1987). Plasmid DNA was purified from E. coli MC1061 by standard procedures (Maniatis et al., 1982) and dialyzed against distilled water prior to use.

Electro-competent cells. Osmotically sensitive cells (OSC) were prepared according to Laurila et al. (1985) with the following modifications: cellophane sheets placed on Potato Dextrose Agar (PDA) plates were inoculated with 5 x 106 spores/ml and incubated for 21 hours at 28 C; the germinative tubes from five cellophane sheets were suspended in 15 ml of buffer (1.2 M MgSO4<
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highest Possible Transformation Efficiencies for High-Throughput Applications
2. New Competent Cells for Highest Transformation Efficiencies
3. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
4. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
5. Transformation of Nematodes With the Helios Gene Gun
6. Transformation of Filamentous Fungi by Microprojectile Bombardment
7. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
10. Optimizing Chemical Transfection and Electroporation of siRNAs
11. High Throughput siRNA Electroporation
Post Your Comments:
(Date:3/5/2015)... (PRWEB) March 05, 2015 BioPlus Specialty ... the position of Vice President of Managed Care. In ... relationships for one of the nation’s leading specialty pharmacies. ... than 15 years of specialty pharmacy and biotech experience ... with Raptor Pharmaceuticals, Vertex Pharmaceuticals, and Pfizer. Prior to ...
(Date:3/5/2015)... SAN DIEGO and TORONTO ... APTO, TSX: APS), a clinical-stage company developing new therapeutics ... cancer, today announced that William G. Rice , ... at the upcoming 27th Annual ROTH Conference on Tuesday, ... the The Ritz Carlton, Laguna Niguel, CA. ...
(Date:3/5/2015)... , March 5, 2015  In response to ... Miami, FL on February 12, ... both of strong financial health and their SUDOSCAN® devices ... research has indicated. Furthermore, Impeto prefers to have the courts ... There are more than 40 articles written by Key ...
(Date:3/5/2015)... 2015 RMI specializes ... orthopedic conditions such as meniscal tears, ACL injuries, ... joint pain due to degenerative conditions like osteoarthritis. ... surgeries to promote better post-surgical outcomes. , Stemnexa ... pain-free bone marrow harvesting with two complimentary cellular ...
Breaking Biology Technology:BioPlus Specialty Pharmacy Announces Hiring of New Vice President of Managed Care: Jamie Damm 2Aptose Biosciences to Present at the 27th Annual ROTH Conference 2Aptose Biosciences to Present at the 27th Annual ROTH Conference 3Impeto Medical Responds to Published Allegations by LD Technology LLC ("LD") 2Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 2Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 3Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 4Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 5
... MUMBAI, July 24 , ... Focus 2009 Annual Executive,MindXchange on Biotechnology Applications in the Food ... The Indian food processing industry has seen some spectacular product,developments ... baby foods. A lot more has to be achieved through ...
... Clocking ... get someone to buddy punch for you, and you,re area access is automatically restricted. ,What,s more - ... ... end to the cat and mouse game of sneaking in late to work - the mouse is ...
... concepts of a thousand words- now, for the first time, ... concepts in our minds. The team, including Professor Rodrigo ... of Leicester in the UK, Professor Itzhak Fried at the ... California Institute of Technology, has published these findings in the ...
Cached Biology Technology:Growing Awareness and Increased Investments Lead to Growth of Biotechnology Applications in the Food & Beverage and Dietary Supplements Sector, Reveals Frost & Sullivan 2Growing Awareness and Increased Investments Lead to Growth of Biotechnology Applications in the Food & Beverage and Dietary Supplements Sector, Reveals Frost & Sullivan 3Growing Awareness and Increased Investments Lead to Growth of Biotechnology Applications in the Food & Beverage and Dietary Supplements Sector, Reveals Frost & Sullivan 4Time Clocks Technology Catches Up With Crooks 2Time Clocks Technology Catches Up With Crooks 3Oprah, Luke Skywalker and Maradona -- new study investigates how our brains respond to them 2Oprah, Luke Skywalker and Maradona -- new study investigates how our brains respond to them 3
(Date:2/10/2015)... Feb. 10, 2015  Alere Inc. (NYSE: ALR), ... financial results for the quarter ended December 31, ... and President of Alere said, "We made substantial ... as the global leader in rapid diagnostics and ... Alere Health divestiture in early January enabled us ...
(Date:2/5/2015)... North Carolina , 5. Februar 2015 ... Jahr als spezialisiertes Logistikunternehmen und hat eine ... einzige klinische Logistikfirma (Clinical Logistics Organization – ... der neuen Kampagne lautet First , ... Foto: http://photos.prnewswire.com/prnh/20150205/173753 ...
(Date:2/5/2015)... SAN DIEGO , Jan. 26, 2015   Epic ... treatment of cancer, today announced that Murali Prahalad , ... Personalized Medicine World Conference (PMWC) 2015: Silicon Valley, ... Mountain View, Calif. on January 26-28, ... entitled, "CTCs Come of Age as Biomarkers." Last year, Epic ...
Breaking Biology News(10 mins):Alere Inc. Announces Fourth Quarter 2014 Results 2Alere Inc. Announces Fourth Quarter 2014 Results 3Alere Inc. Announces Fourth Quarter 2014 Results 4Alere Inc. Announces Fourth Quarter 2014 Results 5Alere Inc. Announces Fourth Quarter 2014 Results 6Alere Inc. Announces Fourth Quarter 2014 Results 7Alere Inc. Announces Fourth Quarter 2014 Results 8Alere Inc. Announces Fourth Quarter 2014 Results 9Alere Inc. Announces Fourth Quarter 2014 Results 10Alere Inc. Announces Fourth Quarter 2014 Results 11Alere Inc. Announces Fourth Quarter 2014 Results 12Alere Inc. Announces Fourth Quarter 2014 Results 13Alere Inc. Announces Fourth Quarter 2014 Results 14Alere Inc. Announces Fourth Quarter 2014 Results 15Alere Inc. Announces Fourth Quarter 2014 Results 16Alere Inc. Announces Fourth Quarter 2014 Results 17Alere Inc. Announces Fourth Quarter 2014 Results 18Alere Inc. Announces Fourth Quarter 2014 Results 19Alere Inc. Announces Fourth Quarter 2014 Results 20Alere Inc. Announces Fourth Quarter 2014 Results 21Alere Inc. Announces Fourth Quarter 2014 Results 22Alere Inc. Announces Fourth Quarter 2014 Results 23Alere Inc. Announces Fourth Quarter 2014 Results 24Alere Inc. Announces Fourth Quarter 2014 Results 25Alere Inc. Announces Fourth Quarter 2014 Results 26Alere Inc. Announces Fourth Quarter 2014 Results 27Alere Inc. Announces Fourth Quarter 2014 Results 28Alere Inc. Announces Fourth Quarter 2014 Results 29Alere Inc. Announces Fourth Quarter 2014 Results 30Alere Inc. Announces Fourth Quarter 2014 Results 31Alere Inc. Announces Fourth Quarter 2014 Results 32Alere Inc. Announces Fourth Quarter 2014 Results 33Alere Inc. Announces Fourth Quarter 2014 Results 34Alere Inc. Announces Fourth Quarter 2014 Results 35Alere Inc. Announces Fourth Quarter 2014 Results 36
... from a simple effect: As the pulp inside dries, the ... Now, a team of researchers at MIT has discovered a ... orderly way, creating wrinkled surfaces with precise sizes and patterns. ... a wide variety of useful structures: microfluidic systems for biological ...
... NJAugust 1, 2012John Wiley & Sons, Inc., today ... publication, WIREs Energy and Environment , publishing ... energy research demands collaboration between different disciplines of ... physical and life scientists, economists, sociologists and policy-makers. ...
... diamond-back moth. It lays its eggs on the cabbage plants ... However, Morten Emil Mldrup from the University of Copenhagen has ... his spectacular research results at a public PhD defense on ... cheat the diamond-back moths to lay their eggs on tobacco ...
Cached Biology News:Wrinkled surfaces could have widespread applications 2Wrinkled surfaces could have widespread applications 3Wiley launches new interdisciplinary review WIREs Energy and Environment 2Gene technology helps deceive greedy pest insects 2