( cells transfected under optimal conditi...)Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

cells transfected under optimal conditions for either LipofectAMINE or LipoTAXI transfection reagents.⁴ Under all conditions tested, LipoTAXI transfection reagent was significantly more effective in transfecting the pEGFP-C3 vector into SW13 cells than LipofectAMINE reagent. Quantitative analysis of transfection efficiencies using various conditions for both liposome-based reagents is summarized in figure 2.

Figure 2

The increased transfection efficiency seen when using LipoTAXI transfection reagent creates a population of cells with various levels of exogenous protein expression. For some of our constructs, we have seen that expression level has a significant effect on the subcellular distribution of the exogenous protein (unpublished observations). Other effects of exogenous proteins, such as enzyme activity, may also be dependent on expression level. Use of LipoTAXI reagent provides a population of transfected cells containing diverse levels of expression of exogenous protein that may enhance studies.

We have concluded that LipoTAXI transfection reagent is less toxic to cells than LipofectAMINE reagent. This conclusion is based on our observation that cell death, as indicated by floating cells, occurred with greater frequency with increasing concentrations of LipofectAMINE reagent. This effect was not seen with increasing concentrations of LipoTAXI reagent. In addition, cell death was much more pronounced in areas where cell density was lower than 50%. This observation may be important for investigators who perform transfections with cell populations that are below confluency, such as those studying cell-cell adhesion.

Conclusions

Our results show that LipoTAXI transfection reagent is significantly more effective than LipofectAMINE reagent for transfe
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