Navigation Links
Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

evels of expression of exogenous proteins typical of transiently transfected cells. It was therefore necessary to optimize our transfection experiments in order to produce enough positively transfected cells for data analysis. In an attempt to overcome these difficulties, we compared the effectiveness of two liposome-based transfection reagents in SW13 cells using pEGFP-C3 as a control vector.

Transient Transfection of the pEGFP-C3 Vector into SW13 Cells

SW13 cells (8 x 104) were plated in a 24-well culture dish and allowed to attach overnight so that the cell density was between 60% and 80% confluent. In order to compare the relative efficiencies of the two liposome-mediated reagents, we used the manufacturers recommendations for concentration of reagent and DNA as a starting point for our experiments. Additional transfections were performed that used more or less of either the reagent or DNA. All procedures were performed according to the manufacturers recommendations, including the use of serum-free media for LipofectAMINE reagent-mediated transfections and serum-enriched media when adding LipoTAXI transfection reagent and DNA solutions to SW13 cells. Cells were exposed to the DNA-liposome complex for 5 hours at 37C in a humidified incubator with 5% CO2. Serum-containing medium was added to the cells, and the cells were incubated overnight. Cells were then fixed with 4% paraformaldehyde at room temperature for 25 minutes and rinsed with deionized, distilled water. Coverslips with attached cells were mounted in glycerol containing 2.5% 1,4-diazabicyclo(2.2.2)octane (DABCO) then viewed and photographed using a Nikon Microphot FX light microscope. Transfection efficiency was determined by counting fluorescent and total cells from six random fields for each condition.

Figure 1 shows fluorescent images of
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
4. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
5. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
6. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
7. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
8. Efficient Transfection of Neurospora Crassa
9. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
(Date:11/21/2014)... Alto, Calif. (PRWEB) November 20, 2014 ... a nonprofit, public policy research organization representing leading ... pharmaceutical firms, and PwC US today ... states in life sciences industry growth. The trend ... 2013. The 2015 California Biomedical Industry Report ...
(Date:11/21/2014)... 2014 2014 Deep Research Report ... a professional and in-depth research report on the ... information, like its definition, classification, application, and industry ... report covers the international market analysis, including China’s ... the US, Europe, Asia, China, Japan etc. regions) ...
(Date:11/21/2014)... Nov. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the ... periods ended September 30, 2014.  Biorem,s complete 2014 third quarter financial ... Financial Summary:Three-months ended September 30, , Nine-months ... data) , 2014 , 2013 , ... , 5,281 , 6,715 , 14,476 ...
(Date:11/18/2014)... Athena Signature Series: Turning Cancer ‘Off’ – featuring Dr. ... in the Regulatory Biology Laboratory at the Salk Institute, studies ... an “off switch” for drug resistance in cancer. , Learn ... is making a global impact in the fight against cancer. ... Cheryl K. Goodman, CEO of Social Global Mobile LLC, for ...
Breaking Biology Technology:California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 2California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 4Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 2Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 3Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3
... launched PATHT HTS, a new platform for high-throughput quantitative ... microarray industry's annual conference, Chips To Hits in Boston. ... performance of PATH protein microarray slides," said Robert Negm, ... "PATH HTS was designed to meet the pharmaceutical ...
... Madison, Wis. -- Up close, Imago Scientific ... steel, glowing ports and snaking hoses and wires. This microscope ... matter does so by an intricate process, first plunging specimens ... them one by one. The atoms next fly to a ...
... Inc. of Norcoss, Ga., will offer the Microsoft Education ... in the metro Atlanta area, the two companies ... a variety of public classes from end user applications to ... , Saint Michael's Hospital in Stevens Point and ...
Cached Biology Technology:UW-Madison helps Imago carry out its big vision for seeing small 2UW-Madison helps Imago carry out its big vision for seeing small 3UW-Madison helps Imago carry out its big vision for seeing small 4UW-Madison helps Imago carry out its big vision for seeing small 5Tech Digest: Inacom; St. Michael's, St. Clare's hospitals; Merge eMed; Advanced Data-Comm; Blue Cross and Blue Shield 2
(Date:11/21/2014)... 2014  Earlier this year Donald Spector , ... one of the most prolific inventors in the world, ... from Smartphones to third party agencies. Spector envisioned this ... of the earliest known patents in this area. Now ... military, child care, elder care and hospital applications. This ...
(Date:11/18/2014)... , Nov. 18, 2014  The Secure Identity ... Association today jointly announce the formation of The ... of its Identity and Biometric Entry and ... Framework received official support from BORDERPOL, the international ... improve and provide expertise regarding border security, traveler ...
(Date:11/15/2014)... 13, 2014  While we may still be a few ... in "Star Trek" to gain instant access to all that ... with smartphones and tablets for monitoring and measuring our health ... This may seem a tad Orwellian to some, but a ... of these technological opportunities into their healthcare regime. ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3Americans May Be Ready for a Brave New World of Healthcare 2Americans May Be Ready for a Brave New World of Healthcare 3Americans May Be Ready for a Brave New World of Healthcare 4
... of the brain and spine in America,s babies, particularly those of Hispanic origin, ... the B vitamin folic acid, according to a new petition filed with the ... Gruma Corporation Spina Bifida ... of Dimes Foundation , ...
... perennially covered in ice. It is exposed to severe ... and, during the winter, 24-hour darkness. But, for the ... To study them, Dr. Rachel Morgan-Kiss from the University ... to sample the ice-covered lake. The article describing her ...
... Academy at the University of Gothenburg, Sweden, have ... osteoporosis. The study, published in Nature Genetics ... shows among other interesting facts that women with a ... a more than 50 percent increased fracture risk. ...
Cached Biology News:Coalition petitions FDA to fortify corn masa flour with folic acid 2Coalition petitions FDA to fortify corn masa flour with folic acid 3Research on carbon-consuming life-forms in Antarctica published in JoVE 2Scientists have discovered genes that increase the risk of osteoporosis and fractures 2
PI/RNase Staining Buffer Solution , 100 ml Consult technical datasheet for details....
Selenoprotein P Purified Anti-Mouse clone 35, Isotype Mouse IgG 2b , 50 µg Consult technical datasheet for details....
Anti-Mouse Reticular Fibroblast, Purified (Clone ER-TR7) (rat IgG2a)...
...
Biology Products: