( results. ...)Transfection Reagent Selector Kit Handbook

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Selector Protocol for Effectene Reagent and SuperFect Reagent

QIAGEN has developed the following protocol for testing Effectene Reagent and SuperFect Reagent in parallel in 6-well plates. The selector kit includes quantities of each QIAGEN Transfection Reagent sufficient to perform two sets of optimization experiments as described. For transfection in other culture formats, please contact QIAGEN Technical Services for the appropriate pipetting scheme.

This selector protocol enables you to determine the best reagent and optimal transfection conditions for your cells by testing 9 different transfection conditions for each QIAGEN Transfection Reagent. The transfection mixes contain different amounts of DNA and DNATransfection Reagent complex, and different ratios of DNA to Transfection Reagent. The selector protocol procedure is illustrated schematically in Figure 1 and the corresponding pipetting scheme is provided in Figure 2. Two controls for each QIAGEN Transfection Reagent are also included in the protocol. The Reagent-but-no-DNA control checks whether the reagent was toxic to the cells, and the no-DNA-no-reagent control checks whether the cell culture conditions were correct. The reagent and transfection conditions yielding the highest transfection efficiency should be used for future experiments using the same cell line/plasmid DNA combination. In this protocol, two 6-well plates are used for testing each QIAGEN Transfection Reagent.

Optimal transfection conditions should be determined for every cell line if the highest transfection efficiency with either QIAGEN Transfection Reagent is required. For additional information on
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