( Effectene Transfection Reagent ...)Transfection Reagent Selector Kit Handbook

>Effectene Transfection Reagent Effectene Transfection Reagent is based on a proprietary non-liposomal lipid. Effectene Reagent works together with a specific DNA-condensing Enhancer to produce outstanding transfection efficiencies. In the first step of EffecteneDNA complex formation, the DNA is condensed by interaction with the Enhancer in a defined buffer system. Effectene Reagent is then added to the condensed DNA to produce condensed EffecteneDNA complexes. The EffecteneDNA complexes are mixed with medium and directly added to the cells.

Effectene Reagent spontaneously forms micelle structures that show no size or batch variation, as found with preformulated liposome reagents. This unique feature ensures excellent reproducibility of transfection complex formation. The process of highly condensing DNA molecules and then coating them with Effectene Reagent is a particularly effective way to transfer DNA into eukaryotic cells.

SuperFect Reagent and Effectene Reagent yield significantly better transfection results than many widely used liposome transfection reagents. SuperFect and Effectene Reagent are suitable for efficient transfection of a wide variety of cell lines (Table 1). Effectene Reagent is also the reagent of choice for primary cells. SuperFect Reagent and Effectene Reagent have been designed to efficiently transfect cells in the presence of serum, thereby reducing stress on the cells and lowering cytotoxicity. In addition, Effectene Reagent requires significantly less DNA to obtain high levels of transfection than do many liposome-based reagents and other transfection methods.

SuperFect Reagent and Effectene Reagent both offer:
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