( in this handbook is designed t...)Transfection Reagent Selector Kit Handbook

in this handbook is designed to test both reagents and different transfection conditions in parallel in order to determine the optimal reagent and transfection conditions for your cells. The selector kit protocol is designed for 6-well plates. If you prefer to use a different culture format for your determination, please contact QIAGEN Technical Services for the appropriate pipetting scheme.

If you prefer to test or use the QIAGEN Transfection Reagents separately, please contact QIAGEN Technical Services or your local distributor for a copy of the Effectene Transfection Reagent Handbook or the SuperFect Transfection Reagent Handbook, or visit us at www.qiagen.com/literature/index.html to obtain the handbooks as Adobe PDF files. Whatever your transfection needs, QIAGEN has the answer.

SuperFect Transfection Reagent

SuperFect Transfection Reagent represents a new class of activated-dendrimer transfection reagent designed for outstanding transfection results (1). SuperFect Reagent possesses a defined spherical architecture, with branches radiating from a central core and terminating at charged amino groups. SuperFect Reagent assembles DNA into compact structures, optimizing the entry of DNA into the cell. SuperFectDNA complexes possess a net positive charge that allows them to bind to negatively charged receptors (e.g. sialylated glycoproteins) on the surface of eukaryotic cells. Once inside the cell, SuperFect Reagent buffers the lysosome after it has fused with the endosome, leading to pH inhibition of lysosomal nucleases. This ensures stability of SuperFectDNA complexes and the transport of intact DNA to the nucleus. '"/>

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