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Transfection Reagent Selector Kit Handbook

illation counter. Currently, a sandwich-ELISA is also often used, which involves an antibody coupled ELISA plate. The hGH protein binds to the antibody on the plate, a digoxygenated antibody binds to hGH, and a secondary antibody coupled to alkaline phosphatase is used for detection.

Green fluorescent protein

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria (3), has the ability to absorb blue light and emit green light. This unique protein can be expressed in mammalian cells and protein expression can be visually monitored in living cells. Although the system provides a convenient way to detect protein expression without a specific assay, quantitative analysis is limited. This reporter gene system is best suited for in situ detection of gene expression, such as localization studies of fusion proteins within cells.

References

  1. Tang, M.X., Redemann, C.T., and Szoka, Jr F.C. (1996) In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjugate Chem. 7, 703714.
  2. Freshney, R.I. (1994) Culture of Animal Cells, A Manual of Basic Technique, Third Edition, Wiley-Liss.
  3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds. (1991) Current Protocols in Molecular Biology, Wiley Interscience, New York.
  4. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. (1989) Molecular cloning a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press.

Product Use Limitations'"/>

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