( Of the variety of di...)Transfection Reagent Selector Kit Handbook

Of the variety of different transfection methods described in literature (3, 4), the DEAEdextran method, the calcium-phosphate method, electroporation, and liposome-mediated transfection are the most commonly used. Each individual method has its characteristic advantages and disadvantages and the choice of transfection method strongly influences transfection results. SuperFect Transfection Reagent based on activated dendrimer technology, and Effectene Transfection Reagent representing a completely new class of lipid-based transfection reagent, have been designed to offer very high transfection efficiencies and reproducibility, while minimizing cytotoxic effects.

Plasmid DNA quality

The quality of plasmid DNA strongly influences the results of transfection experiments. Therefore only plasmid DNA of the highest quality, which is completely free of contaminating RNA, genomic DNA or proteins, should be used. DNA purified with QIAGEN and QIAfilter Plasmid Kits is ideally suited for transfection of most cell lines. For transfection of endotoxin-sensitive cells, we recommend using DNA purified with EndoFree Plasmid Kits. These kits efficiently remove bacterial lipopolysaccharide molecules during the plasmid purification procedure, ensuring optimal transfection results.

Genetic Reporter Systems

After cloning a gene of interest, transfection is a useful tool to determine how cis-acting sequences, such as promoters and enhancers, and trans-acting factors, such as transcription factors, act together to control eukaryotic gene expression. Common methods to monitor gene expression involve using techniques such as
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