( in cell cultures or cell lines grow as...)Transfection Reagent Selector Kit Handbook

in, cell cultures or cell lines grow as an adherent monolayer or in suspension. Cells or cell lines vary greatly with respect to their growth behavior and nutritional requirements (2). Optimization of cell culture technique is necessary to ensure that cells are healthy and in optimal condition for transfection. For extensive information on culturing of cells, please refer to the manual Culture of Animal Cells (2).

Adherent cells

Adherent cells are anchorage-dependent and propagate as a monolayer attached to the culture vessel. This attachment is essential for proliferation. Most cells derived from tissues are anchorage-dependent with the exception of hematopoetic cells (cells derived from blood).

Suspension cells

Suspension cells are able to survive and proliferate without attachment. Hematopoetic cells, transformed cell lines, and cells from malignant tumors can be grown in suspension.

Primary cell culture

Primary cell cultures arise from the outgrowth of migrating cells from a piece of tissue or by enzymatic, chemical, or mechanical dispersal of the tissue. Primary cell cultures are morphologically most similar to the parent tissue.

Finite cell line

Finite cell lines are formed after the first subculturing (passaging) of a primary cell culture, and can be propagated and subcultured several times.

Continuous cell line

There is a limit to the number of generations that a finite cell line can be propagated. Beyond this limit the fin
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