( decreased transfection...)Transfection Reagent Selector Kit Handbook

, decreased transfection efficiencies may be observed in later experiments. We recommend using cells with low passage number (<50 splitting cycles). Serum variability Variations in serum quality can lead to variation in transfection efficiency. In general, it is advisable to test a small lot of serum from a reputable supplier with a control cell line and assess it before performing transfection experiments. Once a given lot has yielded satisfactory and reproducible results, additional sera from the same lot should be purchased.

Appendix A: Composition of Buffers

Buffer Composition Storage

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1x PBS (phosphate-buffered saline) 137 mM NaCl
2.7 mM KCl
4.3 mM Na2HPO4
1.47 mM KH2PO4
Adjust to a final pH of 7.4 Room temp. 1x TE buffer, pH 7.4 10 mM TrisCl, pH 7.4
1 mM EDTA Room temp.

Appendix B: Background Information
Transfection Principle

Transfection delivery of foreign molecules such as DNA into eukaryotic cells has become a powerful tool for the study and control of gene expression, e.g. for biochemical characterization, mutational analyses, or investigation of the effects of regulatory elements or cell growth
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