( ity is observed remove the tr...)Transfection Reagent Selector Kit Handbook

ity is observed, remove the transfection complexes by centrifugation after a 23 hour incubation period. Remove the medium from the cell pellet, resuspend cells in fresh medium (containing serum and antibiotics), and incubate for gene expression.

For Effectene Reagent:

For adherent and suspension cells, results show that in many cases, removal of transfection complexes is not necessary. However, if cytotoxicity is observed, remove the EffecteneDNA complexes 618 h after addition, wash with PBS, and replace with fresh medium (containing serum and antibiotics), and incubate for gene expression.

Troubleshooting Guide

The following troubleshooting guide is helpful if lower transfection efficiencies or higher cytotoxicity than expected is observed. Comments and suggestions are listed in the order in which they should be considered. Observation Possible Cause Comments and Suggestions

---

Low transfection efficiency Transfection Reagent to DNA ratio is sub-optimal If the ratio of Transfection Reagent to DNA is sub-optimal, the overall charge of the complexes may be negative, neutral or strongly positive, which can lead to inefficient adsorption to the cell surface. Optimize the Transfection Reagent to DNA ratio according to the optimization section. Insufficient amount of Transfection Reagent DNA complex If the transfection ef
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Efficient Transfection of Neurospora Crassa
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. Efficient Transfection of Neurospora Crassa