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Transfection Reagent Selector Kit Handbook

erum and antibiotics, and continue incubation for an appropriate time for gene expression.
Post-transfection we recommend to:
  • Check the cells before evaluation of gene expression - is any toxicity apparent?
  • Check and note the incubation time before harvesting for evaluation of gene expression.

  • To assay for gene expression, proceed as follows:
    For transient transfection, harvest cells and evaluate after an appropriate incubation time. For example, cells transfected with -gal or cat reporter constructs are typically incubated for 2448 h after transfection to obtain maximal expression levels of the reporter gene.

    • Transfection Optimization

    The selector protocol in this handbook enables you to optimize the amount of DNA and Transfection Reagent, and the ratio of DNA to Transfection Reagent for each QIAGEN Transfection Reagent. In addition to these parameters, it is recommended to optimize a number of other parameters in order to achieve optimal transfection efficiency for every new cell line/plasmid DNA combination used. These additional parameters are the cell confluency/density at the time of complex addition and the length of exposure of cells to Transfection ReagentDNA complexes. Once the parameters yielding maximum transfection efficiency have been determined, they should be kept constant in every experiment using a particular cell line/plasmid DNA combination.

    Cell density at the time of complex addition

    For 6-we
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