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Transfection Reagent Selector Kit Handbook

ificantly decrease transfection efficiency.

  • Pipet 100 l of mastermix D into tubes 12, 13, 14, each of which will finally contain 1 g of DNA.
    Pipet 100 l of mastermix E into tubes 15, 16, 17, each of which will finally contain 2 g of DNA.
    Pipet 100 l of mastermix F into tubes 18, 19, 20, each of which will finally contain 4 g of DNA.

  • Controls for SuperFect Reagent
    Reagent-but-no-DNA control: Pipet 100 l of growth medium containing no serum or antibiotics into tube 21.
    No-DNA-no-reagent control: Pipet 100 l of growth medium containing no serum or antibiotics into tube 22.

  • To tubes 19 and control tube 10: add the volume of Effectene Reagent specified in Figure 2 to the condensed DNA solution. Mix by pipetting up and down 5 times, or by vortexing for 10 seconds.
    Note:     It is not necessary to keep Effectene Reagent on ice at all times. 1015 minutes at room temperature will not alter its stability.

  • To tubes 1220 and control tube 21: add the volume of SuperFect Reagent specified in Figure 2 to the DNA solutions. Mix by pipetting up and down 5 times, or by vortexing for 10 seconds.
    Note:     It is not necessary to keep SuperFect Reagent on ice at all times. 1015 minutes at room temperature will not alter its stability.

  • Incubate all tubes for 510 min at room temperature (2025C) to allow complex formation.

  • For adherent cells, while complex formation takes place gently
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