( optimization of transfection ...)Transfection Reagent Selector Kit Handbook

optimization of transfection efficiencies, please refer to the optimization guidelines. Once the parameters yielding maximum transfection efficiency have been determined, they should be kept constant in every experiment using a particular cell line/plasmid DNA combination.

If you prefer to test or use the QIAGEN Transfection Reagents separately, please contact QIAGEN Technical Services or your local distributor for a copy of the Effectene Transfection Reagent Handbook or the SuperFect Transfection Reagent Handbook, or visit us at www.qiagen.com/literature/index.html to obtain the handbooks as Adobe PDF files. Transfection Reagent Selector Kit Procedure Figure 1. Procedure for Transfection Reagent Selector Kit. Figure 2. Pipetting scheme for selector protocol with adherent cells in 6-well plates * It is important to prepare mastermixes AC in the order specified, since direct mixing of the DNA with the Enhancer may lead to precipitation.
If you prefer to use a different culture format for your determination, please contact QIAGEN Technical Services for the appropriate pipetting scheme.

NOTE: For transfection of suspension cells in 6-well format, better results may be obtained by using approximately 80% of the SuperFect Reagent volumes indicated in Figure 2.

Cell-culture preparation

1. Prepare two 6-well plates for Effectene Reagent and label the wells 1 11. Prepare two 6-well plates for SuperFect Reagent and label the wells 1222. The day before transfection, seed 0.9 4.0 x 105 cells (depending on the cell type) per
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