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Transcriptor Reverse Transcriptase


  • Permits the analysis of low-expressed genes and RNA, when limited amounts of sample material are available, using two-step RT-PCR:
In combination with the Expand High Fidelity PCR System, your target can be detected in as little as 1 pg total RNA and 100 fg mRNA.

Increase sensitivity in two-step quantitative RT-PCR

Analyze genes with very low or extremely high expression levels
  • Transcriptor Reverse Transcriptase has a broad dynamic detection range of 12 to 1.2 x 109 copies of in vitro transcribed RNA, allowing linear and accurate quantification over 108-fold range of input RNA (Figure 2).



Result: Transcriptor Reverse Transcriptase enables a broad dynamic detection range of 12 to 1.2 x 109 copies PBGD per capillary, which equates to a linear quantification of over 108-fold of input RNA.

Generate clear and interpretable results
  • Compared to other products, Transcriptor Reverse Transcriptase delivers high fluorescence intensities and well-shaped curves with identical distances for RNA dilutions (Figure 3).



Result: Transcriptor Reverse Transcriptase produces curves with better shape, higher fluorescence intensity, and lower crossing points compared to other reverse transcriptases

Obtain full-length transcripts
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