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Transcriptor Reverse Transcriptase

RNase Inhibitor, Transcriptor Reverse Transcriptase, the FastStart PCR Product Family, and PCR-Grade Nucleotides to guarantee your RNA molecules are optimally protected, transcribed, and amplified.

  • Avoid repeating the same experiments and performing multiple RNA extractions.
  • Celebrate quick results.
Transcriptor Reverse Transcriptase is a new recombinant reverse transcriptase expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity and, very importantly, RNase H activity that degrades RNA in RNA:DNA hybrids. If the RNA template is not degraded after first-strand cDNA synthesis, it can bind to the newly-synthesized cDNA and restrict its accessibility to primers during subsequent PCR amplification. RNase H mediated template destruction can prevent this problem and improve the sensitivity of RT-PCR analysis

Transcriptor Reverse Transcriptase optimally combines the individual enzymatic activities, resulting in a number of unique and beneficial properties:

Increase sensitivity in one- or two-step RT-PCR on conventional thermal cyclers
  • Permits the analysis of low-expressed genes and RNA, when limited amounts of sample material are available, using two-step RT-PCR:
In combination with the new FastStart High Fidelity PCR System, as little as 100 pg of total RNA is detected (Figure 1).



Result: The mouse -actin transcript is detected in as little as 100 pg mouse liver total RNA, by two-step RT-PCR.

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