Navigation Links
Transcriptor Reverse Transcriptase

Increase Power and Sensitivity in cDNA Synthesis with the new Transcriptor Reverse Transcriptase

With the new recombinant Transcriptor Reverse Transcriptase, you will
  • Achieve high sensitivity in one- or two-step RT-PCR
  • Obtain full-length cDNA transcripts
  • Reverse transcribe difficult templates with high secondary structure
  • Efficiently label RNA
Transcriptor Reverse Transcriptase is the product of choice to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro transcribed RNA) from a variety of sources for the following applications:
  • Synthesis of first-strand cDNA for use in subsequent amplification reactions, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler Instrument)
  • RT-PCR of GC-rich RNA templates with high secondary structure
  • Incorporation of Cy3-, Cy5-, DIG-, Biotin-, and aminoallyl-modified nucleotides during cDNA synthesis (e.g., for use in microarray applications)
  • Retrieval and cloning of the 5- and 3-termini of mRNA by RACE
  • Generation of cDNA libraries with large inserts
Transcriptor Reverse Transcriptase will allow you to Get the most out of your precious RNA samples
  • Improve the overall performance of existing assays.
  • Perform assays that were not previously possible.
Enjoy working with RNA

  • Discover the most powerful combination available today.
Combine: Protector RNase Inhibitor, Transcriptor Reverse Transcriptase, the FastStart PCR Product Family, and PCR-Grade Nucleotides to guarantee your RNA molecules are optimally protected, transcribed, and amplified.

  • Avoid repeating the same experiments and performing multiple RNA extractions.
  • Celebrate quick results.
Transcriptor Reverse Transcriptase is a new recombinant reverse transcriptase expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity and, very importantly, RNase H activity that degrades RNA in RNA:DNA hybrids. If the RNA template is not degraded after first-strand cDNA synthesis, it can bind to the newly-synthesized cDNA and restrict its accessibility to primers during subsequent PCR amplification. RNase H mediated template destruction can prevent this problem and improve the sensitivity of RT-PCR analysis

Transcriptor Reverse Transcriptase optimally combines the individual enzymatic activities, resulting in a number of unique and beneficial properties:

Increase sensitivity in one- or two-step RT-PCR on conventional thermal cyclers
  • Permits the analysis of low-expressed genes and RNA, when limited amounts of sample material are available, using two-step RT-PCR:
In combination with the new FastStart High Fidelity PCR System, as little as 100 pg of total RNA is detected (Figure 1).



Result: The mouse -actin transcript is detected in as little as 100 pg mouse liver total RNA, by two-step RT-PCR.

  • Permits the analysis of low-expressed genes and RNA, when limited amounts of sample material are available, using two-step RT-PCR:
In combination with the Expand High Fidelity PCR System, your target can be detected in as little as 1 pg total RNA and 100 fg mRNA.

Increase sensitivity in two-step quantitative RT-PCR

Analyze genes with very low or extremely high expression levels
  • Transcriptor Reverse Transcriptase has a broad dynamic detection range of 12 to 1.2 x 109 copies of in vitro transcribed RNA, allowing linear and accurate quantification over 108-fold range of input RNA (Figure 2).



Result: Transcriptor Reverse Transcriptase enables a broad dynamic detection range of 12 to 1.2 x 109 copies PBGD per capillary, which equates to a linear quantification of over 108-fold of input RNA.

Generate clear and interpretable results
  • Compared to other products, Transcriptor Reverse Transcriptase delivers high fluorescence intensities and well-shaped curves with identical distances for RNA dilutions (Figure 3).



Result: Transcriptor Reverse Transcriptase produces curves with better shape, higher fluorescence intensity, and lower crossing points compared to other reverse transcriptases

Obtain full-length transcripts during cDNA synthesis

  • Reverse transcription of mRNA up to 14 kb in length is possible, making it the product of choice for very long eukaryotic mRNA (Figure 4).
  • To achieve high sensitivity and yield in the subsequent PCR, use FastStart Taq DNA Polymerase or the new FastStart High Fidelity Enzyme Blend (both products allow hot-start PCR). The Expand Long Template PCR System is recommended for the amplification of long fragments (> 5 kb).



Result: Transcriptor provides high sensitivity and yield when reverse transcribing very long messages

Enjoy the power to reverse transcribe the most difficult templates
  • Due to the very high thermostability of Transcriptor Reverse Transcriptase (up to 65C), robust reverse transcription of GC-rich RNA with high secondary structure can now be easily achieved (Figure 5).
  • In combination with FastStart Taq DNA Polymerase and the new FastStart High Fidelity Enzyme Blend, Transcriptor Reverse Transcriptase is the product of choice to reverse transcribe and amplify difficult RNAs




Result: Transcriptor Reverse Transcriptase has very high thermostability and can be used at temperatures up to 65C

Shorten the reaction time
  • No need to perform an additional RNase H incubation step after reverse transcription (as is recommended for maximum sensitivity when using a RNase H--mutated reverse transcr iptase). This time-consuming, expensive step can be eliminated with Transcriptor Reverse Transcriptase.
  • No need to perform an additional RNase H incubation step after reverse transcription (as is recommended for maximum sensitivity when using a RNase H--mutated reverse transcriptase). This time-consuming, expensive step can be eliminated with Transcriptor Reverse Transcriptase.

Efficiently label RNA
  • Transcriptor Reverse Transcriptase efficiently incorporates modified nucleotides such as Cy3-, Cy5-, aminoallyl-, DIG-, and Biotin-dUTP modified nucleotides, making it an important enzyme in, for example, microarray applications.


Result: Transcriptor Reverse Transcriptase exhibits excellent efficiency for cDNA labeling with Cy3 and Cy5.

Rely on a multi-purpose enzyme, tested in a wide variety of assays
  • Stringent quality criteria are used during the production and manufacturing of Transcriptor Reverse Transcriptase. Each lot is function-tested with conventional thermal cyclers and the LightCycler Instrument, ensuring functionality in end-point and real-time PCR.
  • Additional specifications (e.g., testing for bioburden) increase the safety level when working with RNA.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
2. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
3. Properties of Reverse Transcriptases
4. Reverse transcriptases suitable for one-step RT-PCRb
5. Reverse transcriptases suitable for two-step RT-PCRd
6. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... 23, 2016  The Biodesign Challenge (BDC), a university ... to harness living systems and biotechnology, announced its winning ... New York City . ... showcased projects at MoMA,s Celeste Bartos Theater during the ... MoMA,s senior curator of architecture and design, and ...
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the Industry Network for ... Design Lab . Located in Pasadena, Calif., the Design Lab’s mission is to ... are designed, built and brought to market. , The Design Lab is Supplyframe’s ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case report published today ... a patient who developed lymphedema after being treated for breast cancer benefitted from an ... paradigm for dealing with this debilitating, frequent side effect of cancer treatment. ...
(Date:6/23/2016)... June 23, 2016  Blueprint Bio, a company dedicated ... the medical community, has closed its Series A funding ... . "We have received a commitment from ... we need to meet our current goals," stated ... the runway to complete validation on the current projects ...
Breaking Biology Technology:
(Date:4/15/2016)... , April 15, 2016  A new ... make more accurate underwriting decisions in a fraction ... timely, competitively priced and high-value life insurance policies ... screenings. With Force Diagnostics, rapid testing ... lifestyle data readings (blood pressure, weight, pulse, BMI, ...
(Date:4/13/2016)... CHICAGO , April 13, 2016  IMPOWER physicians ... are setting a new clinical standard in telehealth ... By leveraging the higi platform, IMPOWER patients can ... weight, pulse and body mass index, and, when they ... quick and convenient visit to a local retail location ...
(Date:3/29/2016)... 2016 LegacyXChange, Inc. (OTC: ... SelectaDNA/CSI Protect are pleased to announce our successful effort ... variety of writing instruments, ensuring athletes signatures against counterfeiting ... from athletes on LegacyXChange will be assured of ongoing ... Bill Bollander , CEO states, "By ...
Breaking Biology News(10 mins):