Navigation Links
TransMessenger Transfection Reagent Handbook

followed throughout the procedure.

General handling
Proper microbiological, aseptic technique should always be used when working with RNA. Hands and dust particles may carry bacteria and molds, and are the most common sources of RNase contamination. Always wear latex or vinyl gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin. Change gloves frequently and keep tubes closed. Ensure that all glassware, plasticware, and solutions (including water and culture medium) are RNase-free.

Disposable plasticware
The use of sterile, disposable, plastic tubes throughout the procedure is recommended. These tubes are generally RNase-free and do not require pretreatment to inactivate RNases.

DNA contamination
No currently available purification method can guarantee that RNA is completely free of DNA, even when no DNA is visible on an agarose gel. For RNA transfection, digestion of the purified RNA with RNase-free DNase is recommended. The RNA can then be repurified using the RNeasy Kit.

Optimizing RNA Transfection

To achieve optimal transfection efficiency for every cell typeRNA combination, we recommend optimizing a number of parameters:

The amounts of TransMessenger Transfection Reagent, RNA, and TransMessengerRNA complexes
The cell number/confluency
The length of exposure of cells to TransMessengerRNA complexes

Once the parameters yielding maximum transfection efficiency have been determined, they should be kept constant in every experiment usi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:11/26/2014)... PMG Research, Inc. announced today ... a network of sites that provide clinical research services to ... patient lives through its partnerships with large physician practices and ... site locations in the Southeastern United States and spans over ... board will be held by: Dr. Robert Holmes, ...
(Date:11/26/2014)... SonaCare Medical, a leading manufacturer of ... participated in the American Urology Association’s (AUA) surgical ... opinion leaders in urology presented on the latest ... masses while attendees had the opportunity to evaluate ... labs. Attendees at the Los Angeles, CA lab, ...
(Date:11/26/2014)... Continuing its award-winning streak, Nerium International, the ... Awards for outstanding creative achievement by its marketing and ... the organization’s top honor, in the Branding category for ... April of this year. The event showcased top speakers ... was awarded a Gold MarCom Award in the Special ...
(Date:11/26/2014)... Global biostimulants market is expected ... an estimated CAGR of 12.5%. In order to ... with least ecological impacts, farmers are looking to ... crop yield more effectively & efficiently. Biostimulants are ... the return on investments. , Leading companies in ...
Breaking Biology Technology:PMG Research Names Medical Advisory Board 2PMG Research Names Medical Advisory Board 3SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 2SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 32019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 4
... Mich., April 15 GeneGo, Inc., the leading,systems ... Science Institute has joined the MetaMiner Cardiac,Consortium. The ... first systems,biology and pathway analysis platform for cardiovascular ... on deliverables that will include,disease specific pathway maps ...
... April 15 WallSt.net,s 3-Minute Press Show is,a ... Tolentino., Shows air Monday through Friday on:, ... Show features in-depth interviews with,public company executives on ... is designed to provide viewers with insight into ...
... /PRNewswire-FirstCall/ - AEterna Zentaris Inc.,(NASDAQ: AEZS ; ... therapy and oncology, today reported the completion of ... its Phase 3 program,in benign prostatic hyperplasia (BPH), ... product candidate, cetrorelix. The study,involves approximately 600 patients ...
Cached Biology Technology:The Scripps Translational Science Institute Joins The MetaMiner Cardiac Consortium 2Featured Stocks on Today's Edition of WallSt.net's 3-Minute Press Show: NPHC, PECD, PNRR 2Featured Stocks on Today's Edition of WallSt.net's 3-Minute Press Show: NPHC, PECD, PNRR 3AEterna Zentaris Completes Patient Recruitment for First Phase 3 Efficacy Trial with Cetrorelix in Benign Prostatic Hyperplasia 2AEterna Zentaris Completes Patient Recruitment for First Phase 3 Efficacy Trial with Cetrorelix in Benign Prostatic Hyperplasia 3AEterna Zentaris Completes Patient Recruitment for First Phase 3 Efficacy Trial with Cetrorelix in Benign Prostatic Hyperplasia 4
(Date:11/10/2014)... shelves in early 2010, and people have used them ... be tossed into a washing machine without ever having ... though, has come with risks for young children. , ... found that from 2012 through 2013, U.S. poison control ... years of age swallowing, inhaling, or otherwise being exposed ...
(Date:11/7/2014)... CORAL SPRINGS, Florida , November 6, 2014 ... improved secure authentication for better mobile security revolutionizing online transactions. ... NXTD ), Alibaba Holdings Ltd. (NYSE: BABA ), ... MSFT ), eBay Inc. (NASDAQ: EBAY ... (NASDAQ: NXTD and NXTDW), a biometric authentication ...
(Date:11/6/2014)... asset for a predator. Except when that predator runs so ... relative to its size, is the fastest creature on Earth. ... per second (at about five miles per hour). The fastest ... sprinting gold from the tiger beetle, a person would have ... beetle has a problem. At peak speeds, everything becomes a ...
Breaking Biology News(10 mins):Study finds laundry detergent pods, serious poisoning risk for children 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6The tiger beetle: Too fast to see 2
... New Haven, Conn. It is not just whats in your ... difference between species at least in yeast according to ... Weve known for a while that the protein coding genes ... senior author Michael Snyder , the Cullman Professor of ...
... Today, researchers report for the first time ... are inherited only from the motherare directly linked to ... which highlights the role of mitochondrial genome variation in ... Genome Research ( www.genome.org ). According ...
... part of Biotesting Europe, a new 358,000 biometrics project, ... that future testing procedures and facilities meet the needs ... growing industry. Biometric recognition systems measure unique behavioural ... as varied as iris images, fingerprints, the structure of ...
Cached Biology News:Evolution is driven by gene regulation 2Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes 2UK scientists working to help cut ID theft 2
CREB-2 (H-290)...
Immunogen: Recombinant full length protein, corresponding to amino acids 1-435 of Human AP2M1. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Anti-Dynamin Monoclonal Antibody Description: 100 g Research Focus: neuroscience Storage: -20C Shipping Temperature: 4C...
cysteine and glycine-rich protein 2,...
Biology Products: