Navigation Links
TransMessenger Transfection Reagent Handbook

IRES will not be efficiently translated due to a lack of factors necessary for translation initiation at an IRES in these cells.

RNA quality
Optimal transfection results are achieved when RNA of the highest purity is used for transfection. Therefore only RNA of the highest quality, which is free of contaminating DNA and proteins, should be used. RNA purified with RNeasy Kits and Oligotex mRNA Kits is highly recommended for transfection. We strongly recommend checking RNA quality before starting the transfection. RNA concentration and purity can be determined by measuring the absorbance at 260 nm (A260) and 280 nm (A280) using a spectrophotometer. An absorbance of 1 unit at 260 nm corresponds to ~40 g RNA per milliliter. RNA integrity and size can be checked by denaturing agarose-gel electrophoresis and ethidium bromide staining. The RNA should appear as a sharp band on the stained gel. If the band is not sharp but appears as a smear containing smaller sized RNAs, it is likely that the RNA has suffered major degradation during its preparation. The functionality of the transcript can be determined by in vitro translation.

Handling RNA
Ribonucleases (RNases) are very stable and active enzymes that do not generally require cofactors to function. Since RNases are difficult to inactivate and minute amounts are sufficient to destroy RNA, do not use any plasticware, glassware, or solutions without first eliminating possible RNase contamination. Great care should be taken to avoid inadvertently introducing RNases during the transfection procedure. In order to create and maintain an RNase-free environment when working with RNA, the precautions listed below must be
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/15/2014)... (PRWEB) January 15, 2014 Cynvenio, a ... management through the genomic analysis of tumor cells in ... Finder™, a web-based cancer decision support application powered by ... with Cynvenio’s ClearID™ genomic test, Therapy Finder will provide ...
(Date:1/15/2014)... 15, 2014 A study has been launched ... Formula 1 track could help to tackle the problem of ... Stowhealth (a GP surgery based in Stowmarket) and academics at ... Telemetry technology, which is inspired by equipment used ...
(Date:1/15/2014)... Raleigh, NC (PRWEB) January 15, 2014 ... enhancement to its Online Web Portal for Life Science organizations ... ability to specify the subject matter of their documents in ... This will help reduce time-to-delivery of translations, often a critical ...
(Date:1/15/2014)... Jan. 15, 2014 TaiGen Biotechnology Company, Limited ("TaiGen") ... with R-Pharm, a leading Russian pharmaceutical company, to develop ... Russian Federation , Turkey ... (CIS). Nemonoxacin is a novel antibiotic for the treatment of ...
Breaking Biology Technology:Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4
... has traditionally been a face-to-face interaction in which people ... ,Since the technology boom, the networking landscape has changed ... list servs and online social networking. People no longer ... relationships. , ,The Midwest has been generously affected ...
... bacteria that causes tuberculosis (TB) and leprosy are notoriously ... held in check for the past 50 years by ... therapy. , ,Now, however, a new chink has been ... diseases difficult to treat. The discovery, reported today (May ...
... committed to helping improve patient safety and healthcare through ... administration and the efforts of Secretary Thompson and his ... If you are interested in learning more about this ... ,The University of Wisconsin and UW Health in ...
Cached Biology Technology:Using Technology to Professionally Network in the Midwest 2Using Technology to Professionally Network in the Midwest 3Using Technology to Professionally Network in the Midwest 4UW discovery could lead to new TB treatments 2UW discovery could lead to new TB treatments 3The doctor is in: Thompson prescribes better health information technology 2The doctor is in: Thompson prescribes better health information technology 3The doctor is in: Thompson prescribes better health information technology 4
(Date:4/17/2014)... and fragmentation of forests in the Amazon help ... contributing to rapid and widespread forest loss during ... The findings show that forests in the Amazon ... coupled with forest fires lead to large-scale loss ... Balch, assistant professor of geography, Penn State. , ...
(Date:4/17/2014)... Press journal Current Biology on April 17 ... lives. The Brazilian insects, which represent four distinct but ... the first example of an animal with sex-reversed genitalia. ... several different animals, Neotrogla is the only ... says Kazunori Yoshizawa from Hokkaido University in Japan. , ...
(Date:4/17/2014)... chock full of wild animals and plant life, but ... are churning away in the soil, decomposing organic matter ... vital role these fungi play in ecological systems, their ... of scientists has generated a genetic map of more ... work was published this week in the Proceedings ...
Breaking Biology News(10 mins):Drought and fire in the Amazon lead to sharp increases in forest tree mortality 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 3In sex-reversed cave insects, females have the penises 2Stanford biologists help solve fungal mysteries 2Stanford biologists help solve fungal mysteries 3
... Jan. 28, 2009 Mice with increased levels of a ... diet, researchers at UT Southwestern Medical Center have found. ... to the "weight-loss hormone," leptin, the researchers report. ... useful as a therapy against obesity, said Dr. Masashi Yanagisawa, ...
... and engineers will face a host of obstacles over ... of people caught up in a water shortage crisis, ... a briefing at the Broadcast Center of the National ... Chemical Society,s Global Challenges/Chemistry Solutions. The American ...
... Dog owners who sleep with their pet or permit licks on ... half of owners bond with their pets in these ways. ... these dog owners are no more likely to share the same ... are other dog owners. Dr. Kate Stenske, a clinical assistant ...
Cached Biology News:Natural brain substance blocks weight gain in mice, UT Southwestern researchers discover 2Substantial work ahead for water issues, say scientists at ACS' Final Report briefing 2Substantial work ahead for water issues, say scientists at ACS' Final Report briefing 3Dog owners more likely to share germs with pets by not washing hands than by sleeping with dog 2
20X TE buffer *RNase free*...
...
MOUSE ANTI HUMAN MOC31:AZIDE FREE...
... utility and flexibility press-to-seal silicone isolators can ... the researcher. They are available either pre-cut ... material that can be easily trimmed to ... and non-cytotoxic and can be used to ...
Biology Products: