Navigation Links
TransMessenger Transfection Reagent Handbook

enter Mainz, Germany
Tel: +49-6131-19240.


Introduction

TransMessenger Transfection Reagent has been designed to efficiently transfect eukaryotic cells with RNA, including siRNA (see page 14). This handbook contains guidelines and protocols for the transfection of single-stranded RNA (pages 913) and double-stranded siRNA (pages 1417).

The TransMessenger Principle
TransMessenger Transfection Reagent is based on a lipid formulation and is used in conjunction with a specific RNA-condensing reagent (Enhancer R) and an RNA-condensing buffer (Buffer EC-R). In the first step of TransMessengerRNA complex formation, the RNA is condensed by interaction with Enhancer R in a defined buffer system. In the second step, TransMessenger Transfection Reagent is added to the condensed RNA to produce TransMessengerRNA complexes. The TransMessengerRNA complexes are then mixed with serum-free medium and added directly to cells.

General Considerations for RNA Transfection

Transfection efficiencies are affected by a variety of parameters. The following factors should be considered carefully.

Cell culture
A healthy cell culture lays the foundation for successful transfection, particularly when transfecting cells with RNA. We strongly recommend subculturing cells for a minimum of 24 hours before transfection. This ensures normal cell metabolism and increases the likelihood of RNA uptake. Best results are obtained using the confluence levels indicated in the appropriate protocol sections. Contamination with bacteria (e.g., mycoplasma) and fungi should be avoided, since this can dra
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:3/26/2015)... 26, 2015 PRC Clinical, a Silicon-Valley ... several management and executive appointments effective immediately. , Mike ... (CFO) and member of the Board of Directors. Daniel ... and Sue Dowden will assume the position of Senior ... Chief Operations Officer (COO), has been appointed to the ...
(Date:3/26/2015)... SAN DIEGO , March 26, 2015  BioNano ... the appointment of  Terry Salyer as chief commercial ... building highly effective, worldwide sales teams in life sciences. ... Irys ® System, a genome-mapping platform to assemble ... and investigate structural variations. To date, 32 institutions located ...
(Date:3/26/2015)... 2015 PAREXEL International Corporation (Nasdaq: ... research organization , announced today that the ... acquire all of the business assets of privately-owned ... of specialized pharmacovigilance services, based in Chandigarh, ... assessment, monitoring, and prevention of adverse effects ...
(Date:3/26/2015)... Ale Gicqueau, Founder and President of ... Affairs at Align Technology, on “ Conducting Postmarket Registries ... 2015 Global Conference and Exhibition on April 25th through ... During the poster presentation, Ale Gicqueau and Victor Chen ... Postmarket Registry, the requirements and resources to conduct the ...
Breaking Biology Technology:PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 4Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3
... N.C. A genetic tool used by medical researchers may ... microbes and viruses from drinking water. , In a series ... of genetic material could successfully target a matching portion of ... make it stop working. If this new approach can be ...
... - YM BioSciences Inc.,(AMEX: YMI , TSX: ... and commercializes differentiated products for patients worldwide,today announced ... its,anti-cancer drug nimotuzumab, a humanized monoclonal antibody that ... the 2008 American,Society of Clinical Oncology (ASCO) Annual ...
... presentation at the 14th,International Conference on Prenatal Diagnosis and ... Diagnostic.,I.D.Rh(D) is the first in a series of noninvasive ... maternal blood sample., The I.D.Rh(D) test diagnoses the ... and can be performed as early as ten weeks ...
Cached Biology Technology:Possible new approach to purifying drinking water 2Possible new approach to purifying drinking water 3NIMOTUZUMAB CLINICAL DATA PRESENTED AT ASCO ANNUAL MEETING 2NIMOTUZUMAB CLINICAL DATA PRESENTED AT ASCO ANNUAL MEETING 3NIMOTUZUMAB CLINICAL DATA PRESENTED AT ASCO ANNUAL MEETING 4Presentation at the 14th International Conference on Prenatal Diagnosis and Therapy Highlights Biocept's I.D.Rh(D) Prenatal Diagnostic 2
(Date:3/20/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/vkzvcs/iris_biometrics ) ... Global Strategic Business Report" report to their offering. ... in US$ Thousands. The report provides separate comprehensive analytics for ... , Europe , Asia-Pacific ... Latin America . Annual estimates and ...
(Date:3/17/2015)... 17, 2015  MecklerMedia Corporation (OTCQX: MECK) announced ... service robots ever held in New ... May 11-13, 2015 at the Javits Convention Center. ... event include: Acorn Product Development; Axis NJ; c-Link ... Robotics; Littler; NewBotic Corporation; Neya Systems LLC; Reliabotics; ...
(Date:3/12/2015)... , March 12, 2015 WHEN:Tuesday, March ... Registration here: http://bit.ly/1G7Os0L . SPEAKERS: , Frost ... SeshagiriPhoto - http://photos.prnewswire.com/prnh/20150311/181111 Biometric ... to compete in several different markets and industries. ... currently witnessing an uptrend. Join ...
Breaking Biology News(10 mins):Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5MecklerMedia's RoboUniverse New York Announces Sponsors and Exhibitors, May 11-13, 2015 2Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 2Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 3
... be brought back from the brink with help from ... Kerrie Mengersen, from the School of Mathematical Sciences, is ... the Indonesian primate whose name means "person of the ... a quarter of villagers who lived side-by-side with orangutans ...
... /PRNewswire/ -- PAREXEL International Corporation ... services provider, today announced that it reached a milestone ... during the past three years.  The studies, which were ... more than 3,000 patients.   Early phase studies conducted ...
... results from a study by neuroscientists at Rush University Medical ... exhibit a specific structural change in the brain that can ... identify those who would most benefit from early intervention. ... meeting for the Society of Neuroscience in San Diego, Calif., ...
Cached Biology News:Orangutans count on stats for survival 2PAREXEL Completes 200 Patient Studies In Its Early Phase Units Over Three Years 2PAREXEL Completes 200 Patient Studies In Its Early Phase Units Over Three Years 3PAREXEL Completes 200 Patient Studies In Its Early Phase Units Over Three Years 4MRI scans show structural brain changes in people at risk for Alzheimer's disease 2
E2F-5...
DAP12 Market Segment: Protein Kinases...
Rabbit polyclonal to Flagellin ( Abpromise for all tested applications). Antigen: Synthetic peptide from the flagellin C terminal domain of Burkholderia pseudomallei....
Naphthol Blue Black, CI 20470...
Biology Products: