Navigation Links
TransMessenger Transfection Reagent Handbook

Influence of the RNA Factors such as the size of the RNA and special features contained within it (e.g., IRES, cap, or poly-A tail) can influence its stability and/or the expression rate. Some RNA features may not be compatible with every cell type. Sub-optimal cell
density If cell density at the time of TransMessenger RNA complex addition is not at an optimal
level, cells may not be in the optimal growth phase. This can lead to insufficient uptake of
complexes into the cells or inefficient processing of the RNA of interest. For adherent cells, the optimal confluency for transfection is 8090% (for single-stranded RNA) or 5080% (for siRNA). Be sure to seed cells a minimum of 24 h prior to transfection. Cell typeRNA
combination RNA species with features such as a cap, poly-A tail, or IRES will not necessarily be processed in every cell type. For example, an RNA with an IRES may not be translated in COS-7 and HeLa cells. If no expression is observed change the cell type or, if possible, the RNA. Poor RNA quality RNA used for transfection should be of high quality. Impurities present in RNA preparations can potentially lower transfection efficiency. Degradation of RNA leads to decreased expression levels. RNA should be checked for degradation before transfection. We recommend purifying RNA using RNeasy Kits, Oligotex mRNA Kits, or QIAGEN DNA/RNA Kits. Rep
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/23/2015)... Jan. 23, 2015  Array BioPharma Inc. (NASDAQ: ... definitive agreement with Novartis Pharma AG to acquire worldwide ... Phase 3 development.  This agreement is conditional on the closing ... on April 22, 2014, which are expected to close in ...
(Date:1/22/2015)... January 22, 2015 Shimadzu Scientific ... UV-1280 multipurpose UV-visible spectrophotometer. Offering wavelength scanning from ... is ideal for applications in a variety of ... , The user-friendly UV-1280 enables intuitive operation, while ...
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces ... of office space for Shire a leading biopharmaceutical company, at ... Transwestern | RBJ,s Robert Richards , president, and ... lease for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... January 22, 2015 Protocol Networks brings ... growing team of brand-neutral, independent consultants, Protocol Networks has ... explained that over the years, his company has attracted ... Town of Plainfield, as well as others. With the ...
Breaking Biology Technology:Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 2Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 3Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 4Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 5Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 6Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 7Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2
... WASHINGTON - A delegation led by Governor Doyle and ... week for the biotechnology industrys leading conference with goals ... states research and university community and to let others ... to members of the group, the delegation met its ...
... get difficult, its the companies who dont panic, that will ... years ago, I was working on a project for the ... San Jose, California. I was arranging and conducting seminars for ... again going through a make or break situation with many ...
... WI - GE Medical Systems, a unit of General ... by the U.S. Food and Drug Administration to begin ... (MRI) system. The Signa EXCITE 3.0T combines the increased ... with the breadth of imaging capabilities found on premium ...
Cached Biology Technology:On site at BIO 2003 2On site at BIO 2003 3On site at BIO 2003 4On site at BIO 2003 5A Race without a Finish Line! 2GE Medical Systems Receives FDA Clearance for New MRI System 2
(Date:12/24/2014)... , Dec. 23, 2014  Since its launch in December 2014, the ... eliminate the pain of trying to remember their usernames and ... fused to their smartphones. To assist people who have struggled ... company that created 1U and focuses on redefining identity, announced ...
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ... "Micro Market Monitor: North America Perimeter Security Systems Market" ... The North American perimeter security market is ... to 2019. Although the U.S. market holds a larger share ...
(Date:12/17/2014)... NEW YORK , Dec. 15, 2014  HITLAB SM ... Practices (GCP) audit to confirm its adherence to current ... enables HITLAB to conduct regulated smart device and smart ... safety and research quality. "HITLAB is determined ...
Breaking Biology News(10 mins):1U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2
... may help to protect against some food allergies. Kefir, a ... health food, and is often used to wean babies, as ... in children under the age of three, with around 5-8% ... of the problematic food. , "Friendly" bacteria in kefir may ...
... in the event of a large-scale smallpox bioterrorist attack ... by researchers at Fred Hutchinson Cancer Research Center that ... , Instead, the current U.S. government policy of post-release ... workers and close contacts would be sufficient to thwart ...
... information is passed from parents to offspring via sperm or ... as yeast, the genes can be passed to the next ... occur in the genetic material after it has been duplicated ... mature gametes or spores. Near the end of the process, ...
Cached Biology News:Friendly bacteria in alcoholic milkshake could fight food allergies 2Mass vaccination unnecessary in the event of a large bioterrorist US smallpox attack 2Mass vaccination unnecessary in the event of a large bioterrorist US smallpox attack 3Mass vaccination unnecessary in the event of a large bioterrorist US smallpox attack 4Mass vaccination unnecessary in the event of a large bioterrorist US smallpox attack 5Single molecular 'mark' seen as pivotal for genome compaction in spores and sperm 2
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/L; 1 tube of 1, 000 units enzyme/tube w/Buffer II and MgCl2...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/uL; 25 tubes of 1, 000 units enzyme/tube w/ Buffer I...
A robust formulation of Taq Polymerase supplied with a 10X colored reaction buffer which allows Direct Gel-Loading...
Ps-Aeruginosa (IgA) -Ab EIA Sample Size: 100 l...
Biology Products: