Navigation Links
TransMessenger Transfection Reagent Handbook

y 37C and 5% CO2). 3. On the day of transfection, dilute 1.6 l Enhancer R in Buffer EC-R. Add 0.8 g siRNA (minimum siRNA concentration, 0.1 g/l) and mix by vortexing. The final volume should be 100 l.
IMPORTANT:
Always mix Enhancer R with Buffer EC-R before adding RNA. Always keep the ratio (g:l) of siRNA to Enhancer R constant (1:2). 4. Incubate at room temperature (1525C) for 5 min, and then centrifuge the mixture for a few seconds to collect drops from the top of the tube. 5. Add 4 l TransMessenger Transfection Reagent to the siRNAEnhancer R mixture. Mix by pipetting up and down 5 times, or vortexing for 10 s. 6. Incubate the samples for 10 min at room temperature (1525C) to allow transfectioncomplex formation. 7. While complex formation is taking place, gently aspirate the growth medium from the plate, and carefully wash cells once with sterile PBS using 1.52 times the volume of media used for cell seeding.
IMPORTANT:
Do not allow the cells to become dry. Minimize the time they are without medium. 8. Add 300 l cell growth medium (without serum or antibiotics) to the tube containing the siRNATransMessenger Reagent complexes. Mix by pipetting up and down twice, then immediately add the transfection complexes drop-wise onto the cells. Gently swirl the pla
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:7/29/2014)... TransVac Solutions, the trusted leader in automated ... for hospitals, will be exhibiting at booth #209 at ... & Technical Exhibition Aug. 3-6 in Chicago, Ill. ... hospitals cleaner, reducing infection risk and saving millions in ... Staff will be available at booth #209 to ...
(Date:7/29/2014)... Hill, South Carolina (PRWEB) July 29, 2014 ... with teams of people where cell phone masts or other ... iPhones up to 100 feet away without the need for ... to target longer ranges. , The new app has launched ... of US homes are at very high risk of being ...
(Date:7/29/2014)... Eppendorf Centrifuge 5427 R has ... R is geared towards high-end applications like genomic, atomic, ... towards work requiring high sample throughput. Pipette.com is excited ... their extensive 16, 48, and 64 multichannel pipettes as ... tubes, PCR strip tubes, and more. , Introducing ...
(Date:7/29/2014)... , July 29, 2014 ... System (LBS) zur Erfassung biologischer Daten in ... entwickelt und patentiert. Das LBS nimmt gleichzeitig ... Koronararterie des Patienten und sucht erhöhte biologische ... das Potenzial, die Entdeckung und Entwicklung neuer ...
Breaking Biology Technology:TransVac Presents Cost Saving Technology at ASHE Conference for Keeping Hospitals Cleaner 2DisasterTalk App Could Save Lives as Report Reveals 8% of US Homes at Very High Risk of Natural Disaster 2International Pipette Distributor, Pipette.com Announces the Addition of the Eppendorf Centrifuge 5427 R to Their Product Portfolio 2PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 2PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 3PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 4
... In the merger, each outstanding share of common stock of ... either 0.63 shares of common stock of Bruker Daltonics or, ... to be of equivalent value, payable 75% in Bruker Daltonics ... stock of Bruker Daltonics are not being changed in the ...
... the development and use of devices that are one-billionth ... importance as a science, and the term now describes ... less than 1,000 nanometers of the substances involved in ... in the creation of exciting and innovative new products ...
... labs, technology, research, communication and planning , ,WASHINGTON ... the Department of Health and Human Services has made ... of states, territories and metro areas to respond to ... to receive $25 million, of which almost $16 Million ...
Cached Biology Technology:Bruker Daltonics and Bruker AXS Announce Merger Agreement 2Nanotechnology - Natures Lego Blocks! 2Wisconsin to Receive $25 Million for Bioterrorism 2
(Date:7/29/2014)... overseas territory of Ascension Island, one of the world,s ... renaissance. , Writing in the journal Biodiversity and ... Ascension Island Government Conservation Department report that the number ... outpost has increased by more than 500 per cent ... 24,000 nests are now estimated to be laid on ...
(Date:7/29/2014)... 2014  When it comes to technology, healthcare is usually one ... last. However, when it comes to wearables, healthcare is trying to ... the Internet of Things and its wearable cousins is less than ... healthcare industry, whether it,s in the hospital for a major procedure ... With that in mind, InformationWeek editor Rodney Brown ...
(Date:7/29/2014)... Schools of Dentistry (UM SOD) and Medicine (UM ... a five-year $10.7 million grant award from the ... of the National Institutes of Health to study ... (STDs). The grant, which renews a previous $12 ... direction for the research by studying chlamydial and ...
Breaking Biology News(10 mins):Major turtle nesting beaches protected in 1 of the UK's far flung overseas territories 2University of Maryland Schools of Dentistry and Medicine receive NIH grant 2University of Maryland Schools of Dentistry and Medicine receive NIH grant 3University of Maryland Schools of Dentistry and Medicine receive NIH grant 4
... Valley, NY. (Dec. 20, 2012) When different types ... them aid in repairing central nervous system (CNS) trauma, ... A Belgian research team carried out research aimed at ... cells - neural stem cells, mouse embryonic fibroblasts, dendritic ...
... U.S. Secretary of the Interior Ken Salazars announcement of ... (NPR-A) that balances wildlife conservation and energy development in ... Integrated Activity Plan and Final Environmental Impact Statement (Final ... is the first comprehensive land management plan ever developed ...
... Emeritus of the department of dermatology at Boston University School ... has been named a 2012 Charter Fellow of the National ... End, is among four faculty members at Boston University nominated ... fellows, who are elected by their peers, represent 98 innovators ...
Cached Biology News:Cell Transplantation study investigates fate and function of cells transplanted to the CNS 2WCS applauds Dept. of Interior plan balancing conservation and energy development in NPR-A 2
... Virgin, optically clear polystyrene with frosted ... coordinates for cell counting. 100% integrity ... a new product number, created to ... showing no availability yet, please order ...
Automated Cell Culture Flask...
... 100-240 V, uses multiplex suspension array technology ... up to 100 proteins and peptides in ... is composed of up to 100 color-coded ... conjugated with a unique reactant specific to ...
...
Biology Products: