Navigation Links
TransMessenger Transfection Reagent Handbook

to be transfected and kept constant in future experiments. This is achieved by counting cells prior to seeding and by keeping the time period between seeding and transfection (minimum 24 hours) constant. This will ensure that the cell density is not too high and that the cells are in optimal physiological condition on the day of transfection.

Table 4. Pipetting scheme for optimization of siRNA transfection in 24-well plates

Guidelines for Transfection of siRNA Duplexes Using TransMessenger Transfection Reagent

The procedure below is based on the TransMessenger Reagent standard procedure and RNAi studies using targets described by Elbashir et al. (2). This procedure is provided as a starting point for optimization of siRNA transfection in mammalian cells using TransMessenger Transfection Reagent. For specific cell type and targets, optimal conditions could be different from those described here. Please be sure to read the background information and the protocol notes on pages 9 and 10 of this handbook before starting. 1. The day before transfection, seed 50,000 to 100,000 cells (depending on cell type and the time point of analysis) per well of a 24-well plate in 0.5 ml appropriate growth medium containing serum and antibiotics.
Make sure that cells are in good condition and are seeded 24 h before transfection. Cells should be 5080% confluent on the day of transfection. 2. Incubate cells under their normal growth conditions (typicall
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:9/30/2014)... , Sept. 30, 2014  RegeneRx Biopharmaceuticals, Inc. ... that it has received a Canadian Patent for ... Peptides.  This patent includes claims using thymosin beta ... actin-sequestering peptides for these purposes.  The patent will ... Biopharmaceuticals, Inc. ( www.regenerx.com ) ...
(Date:9/30/2014)... OriginOil Inc. (OTC/QB: OOIL), developer of Electro ... up large quantities of water, announced today that in ... the launch of the company's CLEAN-FRAC™ industrial grade demonstrator ... STW Resources . , Immediately upon startup, the ... and achieved a 99.8% reduction in Turbidity on flowback ...
(Date:9/30/2014)... iLab Solutions announced the results of ... which focuses on core facility operations, growth and utilization ... today. , In its 4th year running, a number ... the year-over-year decrease in the percentage of revenue that ... feeling the constraints of this trend. When asked, “What ...
(Date:9/30/2014)... MA (PRWEB) September 30, 2014 ... (UC) and collaboration company is dedicated to supporting ... connectivity needs. Julie Power, a carrier ... to ensure collaboration solutions stays connected and productive ... to the increased movement of businesses to cloud ...
Breaking Biology Technology:RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2OriginOil, Inc. Report to Shareholders: Blue Gold in West Texas 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 3SoundConnect Expands Carrier Services Division 2
... 16, Shire plc (LSE: SHP, NASDAQ: SHPGY ... U.S. District Court of the Southern District of,New York ... Shire,s patents: U.S. Patent No. 5,968,976 (",976 Patent"); U.S. ... , The lawsuit was filed ...
... and megabase capacity for the next generation sequencing ... febit today announced the official launch ... several biomedical studies of early access customers over ... and deep sequencing coverage for a broad range ...
... tester machine for medical pouches & sachets from Ireland-based manufacturer Sepha. ... ... A leak tester machine for pharmaceutical and medical pouches ... makes its U.S. market debut through U.S. distributor Service Industries ...
Cached Biology Technology:Shire Files Lawsuit Against Barr Laboratories for Infringement of FOSRENOL(R) (Lanthanum Carbonate) Patents 2Automated Sequence Capture for Next-Generation Sequencing Paves the Way for Personalized Medicine 2Automated Sequence Capture for Next-Generation Sequencing Paves the Way for Personalized Medicine 3U.S. Distributor Debut for PAKSCAN - a Pouch & Sachet Leak Tester Machine from Ireland-based Sepha Ltd. 2U.S. Distributor Debut for PAKSCAN - a Pouch & Sachet Leak Tester Machine from Ireland-based Sepha Ltd. 3
(Date:9/30/2014)... tetrachloroethylene (PCE) in drinking water may increase the ... a new study led by a Boston University ... published in the journal Environmental Health , ... among 1,766 women in Cape Cod, Ma., where ... the early 1980s by the installation of vinyl-lined ...
(Date:9/30/2014)... on Earth, but when the climate changes and temperatures ... The amount of time needed to adjust to new ... in the future, reveals a new study from researchers ... journal Proceedings of the Royal Society B . ... of greenhouse gases in the atmosphere. The greenhouse effect ...
(Date:9/30/2014)... in urban and farm runoff in Hawaii is causing ... , The study, published Tuesday in the peer-reviewed ... the runoff ends up in algae that the turtles ... eyes, flippers and internal organs. , Scientists at Duke ... and Atmospheric Administration (NOAA) conducted the study to better ...
Breaking Biology News(10 mins):Contaminated water linked to pregnancy complications, BU study finds 2Shape up quickly -- applies to fish, too! 2Pollution linked to lethal sea turtle tumors 2Pollution linked to lethal sea turtle tumors 3
... University] The cactus, stalwart of the desert, has quite ... found the world over. In a paper published in ... Academy of Sciences , Brown University biologists and colleagues have ... and 10 million years ago and coincided with species explosions ...
... asses are descendants of the original ancestors of the horse ... now in danger of extinction, largely as a direct result ... Walzer,s group has been working together with colleagues in Germany, ... currently restricted to areas in Mongolia, China, India, Iran and ...
... ... cattle in native pasturelands in Brazil,s Pantanal and Cerrado regions can benefit ... Conservation Society. , The technique, which has been adapted ... small areas for shorter periods of time before moving onto other pastures. ...
Cached Biology News:Succulent plants waited for cool, dry Earth to make their mark 2Succulent plants waited for cool, dry Earth to make their mark 3A boring life -- the Asiatic wild ass in the Mongolian Gobi 2Grazing as a conservation tool 2
The F40 pump maintenance kit is used to service one F40 pump of a BioLogic DuoFlow workstation. It includes 2 piston seals, 4 check valves, seal removal tool, and 2 O-rings....
...
...
...
Biology Products: