Navigation Links
TransMessenger Transfection Reagent

t anti-mouse secondary antibody (a-MAP2) and Alexa Fluor 594-conjugated phalloidin, which binds to f-actin and serves here as an expression control.

Data kindly provided by A.M. Krichevsky and K.S. Kosik, Brigham and Womens Hospital, Harvard Medical School, Boston, MA (from Krichevsky, A.M., and Kosik, K.S. (2002) RNAi functions in cultured mammalian neurons. Proc. Natl. Acad. Sci. USA 99, 11926. Copyright 2002 National Academy of Sciences, U.S.A.)

Procedure All TransMessenger Reagent components are provided as ready-to-use solutions. To generate TransMessenger-RNA transfection complexes, simply mix your siRNA with Enhancer R and Buffer EC-R and incubate for 5 minutes at room temperature, then add TransMessenger Reagent and incubate for a further 10 minutes. The complexes are mixed with medium and added directly to the cells. Following a 3 hour incubation, the medium is changed and the cells are incubated until they are ready for analysis.

Optimal transfection results are achieved using high-purity siRNA. The QIAGEN siRNA Oligo Synthesis Service offers custom siRNA oligos, a range of library siRNAs that target specific genes, non-silencing control siRNAs, and a set of siRNAs targeting genes linked with cancer; the Cancer siRNA Oligo Set Version 1.0.
Since the amount of siRNA used is a critical factor for successful transfection, we recommend optimizing the amounts of siRNA and TransMessenger Transfection Reagent for every cell type-siRNA combination. To facilitate this, the reagent is provided with guidelines and starting points for optimization of RNAi experiments.

App
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
2. TransMessenger Transfection Reagent Handbook
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent

(Date:8/26/2015)... , ... August 26, 2015 , ... ... announces today that a second US laboratory is to open in Manhattan, Kansas ... the continued partnership and long-term growth of research and development through collaboration with ...
(Date:8/26/2015)... FRANCISCO , Aug. 26, 2015  Platform ... that it has received a $1.5M Phase II ... (NIH) to further develop its therapeutic agent to ... clinical need in end stage renal disease (ESRD) ... the National Institute of Diabetes and Digestive and ...
(Date:8/26/2015)... Florida , August 26, 2015 ... the biggest factors fueling the optimism in biotech,s ... M&A,s, collaborations and life sciences advancements.  Active biotechnology ... are Pressure BioSciences, Inc. (OTCQB: PBIO), Sequenom, Inc. ... SRPT ), Raptor Pharmaceutical Corp. (NASDAQ: ...
(Date:8/25/2015)... ... August 25, 2015 , ... Vets Plus, Inc. ... Wis. The company has added a AS16 Sharples centrifuge to harvest products after ... bacterial or fungal fermentation process development. , The new centrifuge greatly expands ...
Breaking Biology Technology:MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 2Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 3New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 2New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 3New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 4New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 5New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 6New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 7New centrifuge and fermentor expand product development capacity at Vets Plus 2
... VIENNA, Austria, March 12 f-star, an antibody,engineering ... its unique Modular Antibody Technology, announced today that ... in addition to its,headquarters in Vienna, Austria. The ... home to a number of biotech companies and ...
... March 12 iCAD, Inc. (Nasdaq:,ICAD), an industry-leading ... detection of cancer, today announced that John,DeLucia has ... Assurance and,Regulatory Affairs, reporting to iCAD President and ... years of medical device, in-vitro diagnostics,and biomedical research ...
... REDWOOD CITY, Calif., March 12 Genomic,Health, Inc. ... Scott, Ph.D.,Genomic Health,s Chairman and Chief Executive Officer, ... Healthcare Conference in Miami, FL,on March 19, 2008 ... the live and subsequently archived webcast of the,presentation, ...
Cached Biology Technology:Antibody Engineering Company f-Star Opens Second Research Site in Cambridge, UK 2iCAD Appoints John DeLucia as Vice President of Quality Assurance and Regulatory Affairs 2iCAD Appoints John DeLucia as Vice President of Quality Assurance and Regulatory Affairs 3Genomic Health to Present at Lehman Brothers Global Healthcare Conference 2
(Date:8/6/2015)... 6, 2015  Today, U.S. Congressman Mike ... ™ to learn firsthand how Silicon Valley technologies ... is a world leader in multi-factor identity management ... helping federal agencies and commercial organizations achieve new ... "The vast majority of network breaches can ...
(Date:8/5/2015)... 5, 2015 According to a ... Market by Software, Services, Vertical (BFSI, Airport, IT & Telecom, ... Global Forecast to 2020", published by MarketsandMarkets, defines and ... into various segments. The global PIAM Market is estimated ... Million by 2019, at a CAGR of 14.9 % ...
(Date:8/4/2015)... Aug. 04, 2015 ... has announced the addition of the ... and Service), Sub-Segment (Computer Forensics, Network Forensics, ... Types, Service, Vertical and Region - Global ... offering. By Component (Hardware, Software, ...
Breaking Biology News(10 mins):Rep. Honda to Visit Crossmatch in Redwood City 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2
... for the loss of millions of hours of work productivity this ... fever symptoms, including sneezing, watery eyes and runny and itchy noses, ... peak hay fever season. , While missing an hour of ... million Americans suffer from at least some symptoms related to hay ...
... normal baby. He hit all his developmental landmarks but after his ... with infection after infection. He stopped eating and was no longer ... or even looking at us anymore; he was in his own ... not potty trained, and was smearing (feces) on the walls. My ...
... at University of Copenhagen have identified a new group of ... are published in the new issue of Cell. , All ... specialised cell types that all originate from the same type ... develop into any type of cell through a carefully regulated ...
Cached Biology News:Hay fever can send work productivity down the drain 2Hay fever can send work productivity down the drain 3Army research mission focused on autism 2Army research mission focused on autism 3Army research mission focused on autism 4