t anti-mouse secondary antibody
(a-MAP2) and Alexa Fluor 594-conjugated phalloidin, which binds to f-actin
and serves here as an expression control.
Data kindly provided by A.M. Krichevsky and K.S. Kosik, Brigham and Womens
Hospital, Harvard Medical School, Boston, MA (from Krichevsky, A.M., and
Kosik, K.S. (2002) RNAi functions in cultured mammalian neurons. Proc.
Natl. Acad. Sci. USA 99, 11926. Copyright 2002 National Academy of Sciences,
All TransMessenger Reagent components are provided as ready-to-use solutions.
To generate TransMessenger-RNA transfection complexes, simply mix your siRNA
with Enhancer R and Buffer EC-R and incubate for 5 minutes at room temperature,
then add TransMessenger Reagent and incubate for a further 10 minutes. The
complexes are mixed with medium and added directly to the cells. Following
a 3 hour incubation, the medium is changed and the cells are incubated until
they are ready for analysis.
Optimal transfection results are achieved using high-purity siRNA. The
QIAGEN siRNA Oligo Synthesis Service offers custom siRNA oligos, a range
of library siRNAs that target specific genes, non-silencing control siRNAs,
and a set of siRNAs targeting genes linked with cancer; the Cancer siRNA
Oligo Set Version 1.0.
Since the amount of siRNA used is a critical factor for successful transfection,
we recommend optimizing the amounts of siRNA and TransMessenger Transfection
Reagent for every cell type-siRNA combination. To facilitate this, the
reagent is provided with guidelines and starting points for optimization
of RNAi experiments.
Page: All 1 2 3 Related biology technology :1
. Efficient DNA transfection of primary CNS neurons using TransMessenger
. TransMessenger Transfection Reagent Handbook3
. TransMessenger Transfection Reagent4
. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN
siRNA and TransMessenger Transfection Reagent*5
. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell
. Optimizing Transfection Conditions for Studying Signal Transduction Pathways7
. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells8
. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal
. Versatile Transfection Reagent Offers Low Toxicity and Consistent
. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells11
. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection
of Mammalian Cells