( ...)TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Features and benefits

• Efficient transfection of siRNA for effective gene silencing
  
• Quick and easy setup
  
• Ready-to-use reagent
  
• Tested for absence of RNase activity

Principle TransMessenger Transfection Reagent facilitates effective gene silencing in RNAi procedures by providing efficient transfection of siRNA into eukaryotic cells using a fast and easy procedure (see figure "Efficient Transfection of Primary Neuronal Cells Using QIAGEN siRNA and TransMessenger Reagent"). The reagent is a lipid-based formulation that is used in conjunction with a specific RNA-condensing enhancer and an optimized buffer to form siRNA-TransMessenger Reagent complexes that are efficiently transferred into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and low endotoxin levels (=10 EU/ml). Our rigorous standards eliminate reagent variables that can adversely affect the efficiency of siRNA transfection. Efficient Transfection of Primary Neuronal Cells Using QIAGEN siRNA and TransMessenger Reagent Primary neuronal cells from rats were transfected with a control nonspecific siRNA or an siRNA directed against microtubule-associated protein 2 (MAP2) using TransMessenger Transfection Reagent. Two days after transfection, cells were fixed, permeabilized, and stained using mouse monoclonal anti-MAP2 antibodies and Alexa Fluor 488-conjugated goa
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