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Total RNA from Whole Blood for Expression Profiling

RiboPure-Blood with RNAlater Provides Superior Stabilization and Recovery of RNA
The RiboPure-Blood Kit recovers twice the RNA yield as the market leader's system

RNAlater Stabilization Reagent preserves expression profiles better than competing technologies


Extracting RNA from Blood

Although whole blood is a readily available source for biological sampling, RNA extraction from blood is often difficult. White blood cellsthe most valuable RNA-containing cellsare outnumbered by red cells approximately 1000:1. In addition, once blood is collected, expression levels are threatened by a variety of ex vivo changes, including degradation by potent RNases. Ambion's RiboPure-Blood Kit relies on RNAlater for secure RNA preservation and uses a straightforward RNA isolation method that delivers high yields of quality RNA.


Stabilize and Preserve RNA from Blood Samples

Microarray studies were performed to compare the benefits of RiboPure-Blood KitRNAlater with the market leader's system for isolating blood RNA. Whether the samples were processed immediately or stored at room temperature for three days, RNA yields from blood stored in RNAlater were nearly twice that of samples processed with the competitor's system (Figure 1), and the RNA preparations were of comparable q
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