Navigation Links
Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

Abstract

Applied Biosystems has developed new techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to degradation.

These new techniques respond to the demand for high-quality, highthroughput total RNA extraction methods posed by real-time quantitative PCR and other array-based assays.

Introduction

The ABI PRISM 6700 Automated Nucleic Acid Workstation is a flexible, fully automated system, designed to dramatically increase the throughput of nucleic acid preparation and reaction setup. In just over one hour, the 6700 workstation produces 96 purified samples of total RNA.

In less than 2.5 additional hours, the 6700 workstation prepares up to four fully assembled, 96-well reaction plates containing reagents, standards and controls for 5' nuclease assay analysis or other nucleic acid-based assays.

Chemistry

Applied Biosystems lysis chemistry uses a unique formulation to effectively lyse cells or tissues. The lysis solution is supplied as a 2X formulation and should always be used at a final concentration of 1X. Lysis occurs almost immediately after the cells are placed in the lysis buffer or a tissue sample is homogenized in it. The reagent inactivates cellular RNases, preventing degradation of the isolated nucleic acid, and keeps genomic DNA in solution, allowing a clean separation of RNA from all other cellular components.

The cellular lysate or tissue homogenate is transferred to a purification tray and the lysate or homogenate is passed through the tray under precisely controlled vacuum conditions. The 96-well tray (800 μL/well) contains a glass fibre m
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
(Date:7/2/2015)... -- Spherix Incorporated (Nasdaq: SPEX ) -- an intellectual property development ... property, today announced a litigation update. In ... Corp. et al. , Case No. 1:14-cv-00721-GBL-TCB, in the ... Virginia , the Court issued a ... claims in favor of the Defendants on the one ...
(Date:7/2/2015)... (PRWEB) , ... July 02, 2015 , ... Sleepless nights ... an individual’s quality of sleep, launched its Indiegogo campaign on June 23. Sound sleepers ... voice command technology makes it effortless to use, while the integration of the Bluetooth ...
(Date:7/1/2015)... Costa Mesa, CA (PRWEB) , ... July 01, 2015 , ... ... and traumatic brain injury (TBI) using single photon emission computed tomography (SPECT) ... Columbia. The study, which examined over 20,000 brain scans and is believed to ...
(Date:7/1/2015)... Mass. , July 1, 2015   ... most frequently cited advantages of Otezla are its ... safety profile, as reported by surveyed rheumatologists. Otezla ... launched for the treatment of active psoriatic arthritis ... The current standard of care includes conventional oral ...
Breaking Biology Technology:Spherix Issues A Litigation Update 2Spherix Issues A Litigation Update 3Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3
... Luthersville, GA has the attention of cattle feeders and dairymen industry wide ... put up corn earlage as silage in the high moisture range without ... ... 2008 -- U.S. Ag, LLC of Luthersville, GA has the attention of ...
... Owner of PHC Consulting) hasn't felt a down economy at all. ... in the medical industry (laboratory clinical and research, physician call points, ... for revenues and Peggy attributes that growth to a strong web ... ...
... Entrepreneurial Excellence in Emerging Category, LOS ANGELES, ... announced that Dr. Donald Rice, Founder, President, & ... Year(R) 2008 Award in the,Emerging category in the ... LLP, the award recognizes outstanding entrepreneurs who are ...
Cached Biology Technology:Breakthrough Slashes $7 Corn Over Half for Cattle Feeders 2Breakthrough Slashes $7 Corn Over Half for Cattle Feeders 3Website and Blog Increase Placement for Medical Sales Recruiter - Providing Sales Winners Calling on the Hospital, Lab and Physician Call Point 2Agensys Founder, President, & CEO Dr. Donald Rice Named Ernst & Young Entrepreneur Of The Year(R) 2008 Award Winner for the Greater Los Angeles Area 2Agensys Founder, President, & CEO Dr. Donald Rice Named Ernst & Young Entrepreneur Of The Year(R) 2008 Award Winner for the Greater Los Angeles Area 3
(Date:6/18/2015)... Conn. , June 18, 2015 NXT-ID, Inc. ... authentication company focused on the growing mobile commerce market announces ... a featured segment on "Money on the Mark", scheduled to ... City June 20 th . ... will be from 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO ...
(Date:6/17/2015)... , June 17, 2015 QIAGEN ... launched new Investigator ® STR assay kits for analysis ... United States . The new genetic fingerprint kits provide ... (short tandem repeats or STRs) for DNA matching. They incorporate ... of DNA in each sample, a novel QIAGEN technology that ...
(Date:6/17/2015)... BEACH GARDENS, Fla. , June 17, 2015 ... management solutions, today announced that its U.are.U ® ... Togo,s Eateries, Inc., a ... fast casual sandwich chain, to increase security, improve ... Crossmatch fingerprint readers enable instant, non-repudiable identity confirmation ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3
... AOptix Technologies, Inc., (AOptix) ( www.aoptix.com ), ... and long-distance wireless optical communications solutions, today announced ... AWRE ) will be demonstrating a prototype dual-factor ... exhibition booth at the upcoming Biometrics Consortium Conference ...
... (Santa Barbara, Calif.) Scientists at UC Santa Barbara ... members of a parasitic fluke species that invades ... the early online version of the Proceedings of ... flatworms in colonies with vicious, killer morphs defending the ...
... Irvine, Calif., Sept. 16, 2010 UC Irvine researchers ... and reproduce actually evolve over time. The findings, reported in ... the long-held belief that sexual beings evolve the same way ... genetic research for new pharmaceuticals and other products. "This ...
Cached Biology News:AOptix Technologies to Show Combined Biometric Face and Iris Capture System at Biometrics Consortium Conference 2AOptix Technologies to Show Combined Biometric Face and Iris Capture System at Biometrics Consortium Conference 3'Warrior worms' discovered in snails; UCSB scientists see possible biomedical applications 2UCI scientists decode genomes of sexually precocious fruit flies 2
Human peripheral blood CD19+ B cells, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Monocytes, B Cells, NK Cells and Stem Cells...
Human peripheral blood basophils, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Granulocytes...
Umbilical cord blood CD4+ T cells, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood MNCs and T Cells...
... Isothermal Block is an anodized aluminum, solvent-resistant ... and to provide efficient thermal transfer to ... design of the HT96 Isothermal Block replaces ... product design allowing for compatibility with robotic ...
Biology Products: