Navigation Links
Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

Abstract

Applied Biosystems has developed new techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to degradation.

These new techniques respond to the demand for high-quality, highthroughput total RNA extraction methods posed by real-time quantitative PCR and other array-based assays.

Introduction

The ABI PRISM 6700 Automated Nucleic Acid Workstation is a flexible, fully automated system, designed to dramatically increase the throughput of nucleic acid preparation and reaction setup. In just over one hour, the 6700 workstation produces 96 purified samples of total RNA.

In less than 2.5 additional hours, the 6700 workstation prepares up to four fully assembled, 96-well reaction plates containing reagents, standards and controls for 5' nuclease assay analysis or other nucleic acid-based assays.

Chemistry

Applied Biosystems lysis chemistry uses a unique formulation to effectively lyse cells or tissues. The lysis solution is supplied as a 2X formulation and should always be used at a final concentration of 1X. Lysis occurs almost immediately after the cells are placed in the lysis buffer or a tissue sample is homogenized in it. The reagent inactivates cellular RNases, preventing degradation of the isolated nucleic acid, and keeps genomic DNA in solution, allowing a clean separation of RNA from all other cellular components.

The cellular lysate or tissue homogenate is transferred to a purification tray and the lysate or homogenate is passed through the tray under precisely controlled vacuum conditions. The 96-well tray (800 μL/well) contains a glass fibre m
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
(Date:6/19/2013)... NC (PRWEB) June 19, 2013 ... eclinical commerce network for clinical trials, today announced ... at DIA 2013 (booth #2000) in Boston, June ... the industry’s first and only fully configurable, cloud-based ... ClinPay®, the flagship module within Clinverse’s eClinical Commerce ...
(Date:6/19/2013)... June 19, 2013 MO BIO Laboratories, ... other challenging microbial nucleic acid purification, announces the ... for extraction of pure protein from all soil ... with the 20 th anniversary of the ... (Photo: http://photos.prnewswire.com/prnh/20130619/LA32633 ) ...
(Date:6/19/2013)... CLEVELAND , June 19, 2013   NineSigma ... announced that a year following its launch, NineSights.com ... and a focal point for the worldwide community of ... to climate and environmental sustainability experts, NineSights stands out ... broad range of scientific and technical disciplines, all vetted ...
(Date:6/19/2013)... Today DuPont Executive Vice President James ... in the greatest challenge facing our time – ensuring ... Borel spoke at the International Food and Agribusiness ... need for students to contribute their time and talents ... reached in collaboration with others. , “Food is one ...
Breaking Biology Technology:Clinverse, Inc. Exhibiting and Showcasing Its Technology at DIA 2013 2MO BIO Laboratories, Inc. launches the first kit for protein extraction from soil 2NineSigma Evolves Global Open Innovation through Social Media Platform, NineSights 2NineSigma Evolves Global Open Innovation through Social Media Platform, NineSights 3DuPont Leader Calls for New Generation of Food Visionaries to Fight Hunger 2
... K. H. Valkonen*, N. Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and , ... , * Biotechnology Laboratory, REDEC of Kajaani, University ... ** Department of Biological and Environmental Science, University of Jyvskyl, , ... , Introduction , ...
... Contributed by Philip W Miller, Waltraud I Dunn, and Robert R Schmidt, , ... FL 32611 USA , ... , Introduction , ... elucidate the pre- , and posttranslational mechanisms that regulate ...
... Introduction , ... or probes in genetic analysis. Quality control of these preparations ... electrophoresis or HPLC, but these , methods may ... not provide desired quantitative precision or automation. Capillary electrophorsis , ...
Cached Biology Technology:Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 2Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 3Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 4Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 5Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 6Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 7Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 8Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 9Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 10Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 11Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 12Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B 2Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B 3Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B 4Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B 5Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B 6Oligonucleotide Purity Analysis by Capillary Electrophoresis 2Oligonucleotide Purity Analysis by Capillary Electrophoresis 3
(Date:6/19/2013)... The Microbial Diversity Course at the Marine Biological ... a Milestones in Microbiology site by the American ... in Microbiology program recognizes institutions and the scientists ... toward advancing the science of microbiology. A ... site is scheduled for Saturday, June 22, 2013, ...
(Date:6/19/2013)... , June 19, 2013 MedNet Solutions ... clinical study management systems, is pleased to announce its ... held June 23-27 in Boston . ... a recognized leader in cloud-based eClinical solutions, has exhibited ... be showcasing iMedNet™ EDC , MedNet,s ...
(Date:6/19/2013)... 19, 2013  New York College of Health Professions ... Patent and Trademark Office for detecting biometric changes through ... guards have been injured, attacked or killed. ... the Intellectual Properties Agreements of New York College of ... of New York College says, ...
Breaking Biology News(10 mins):Microbial diversity course designated as a 'Milestones in Microbiology' site 2MedNet Solutions To Spotlight iMedNet EDC At The 49th DIA Annual Meeting 2New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 2New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 3New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 4
... from Huntsman Cancer Institute (HCI) at the University ... to identify cancer-causing rearrangements of genetic material called ... of the method and the research results appear ... Molecular Medicine journal. Many cancers result from ...
... diseases might soon have another warrior to contend with, thanks ... of Illinois. The new antibiotic, an analog of the widely ... boon to the dairy industry as a treatment for bovine ... product of bacteria resident in the cow,s udder. It helps ...
... may have discovered how memories are encoded in ... strengthened synaptic connections among neurons. However components of ... while memories can last a lifetime. Based ... lead researcher Jack Tuszynski, his graduate student Travis ...
Cached Biology News:Beyond the microscope: Identifying specific cancers using molecular analysis 2New antibiotic could make food safer and cows healthier 2New antibiotic could make food safer and cows healthier 3
... The Captivate magnetic ... can be used in conjunction ... (C-21473, C-21474, C-21476) or other ... six 1.5 mL microcentrifuge tubes. ...
Because it is manufactured at our ISO 9001–certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade DAPI is greater than or equal to 98% pure by HPLC....
Edited by D. Patel (1994) Comprehensive data on gel electrophoresis of proteins, nucleic acids, nucleoproteins and carbohydrates....
...
Biology Products: