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Total RNA Isolation From 50100 mg Plant Tissue

tion (300 l will give a concentration of 500 g/ml if the total yield is 150 g RNA).

2. Allow RNA to rehydrate on ice at least 30 min. Vortexvigorously for 5 sec, pulse-spin and carefully transfer sample to a 1.5 ml microfuge tube. Store purified RNA sample at - 7 0 C t o -80C until use.

3. Before use, vortex sample vigorously for 5 sec and pulsespin. Pipet sample up and down several times to ensure adequate mixing.



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