Navigation Links
Total RNA Isolation From 50100 mg Plant Tissue


AquaPure RNA Isolation Kit
Catalog #732-6370
Expected yield range: 50200 g RNA


Method
Cell Lysis
1.Add 50100 mg (0.050.1 g) frozen ground tissue or fresh tissue to a tissue grinder tube on ice and then add 3 ml cell lysis solution. Grind frozen tissue finely in liquid nitrogen with a porcelain mortar and pestle. Keep tissue frozen until added to the tissue grinder tube.

2 . Homogenize quickly using 510 strokes with a tube pestle. Transfer sample to an Oak Ridge centrifuge tube (or other tube rated for high speed).

Protein-DNA Precipitation
1 . Add 1 ml protein-DNA precipitation solution to the cell lysate.

2.Invert tube gently 10 times and place tube into an ice bath for 10 min.

3.Centrifuge at 15,000 x g for 5 min. The precipitated proteins and DNA will form a tight pellet.

RNA Precipitation
1. Pour the supernatant containing the RNA (leaving behind the precipitated protein-DNA pellet) into a clean Oak Ridge centrifuge tube (or other tube rated for high speed) containing 3 ml 100% isopropanol (2-propanol).

2. Cap the tube. Mix the sample by inverting gently 50 times.

3. Centrifuge at 15,000 x g for 5 min; the RNA will be visible as a small, translucent pellet.

4. Pour off supernatant and drain tube on clean absorbent paper. Add 3 ml 70% ethanol. Cap and invert the tube several times to wash the RNA pellet.

5. Centrifuge at 15,000 x g for 2 min. Carefully pour off the ethanol.

6. Invert and drain the tube on clean absorbent paper and allow to air-dry for 15 min.

RNA Hydration
1. Add 300 l RNA hydration solu
'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Total RNA Isolation From Plant Tissue

(Date:7/23/2008)...nt of retraction needed for surgical ...uly 23 /- Pioneer Surgical Technology...imFuse(TM) Anterior,Cervical Plate System. This in...cervical fusion procedures while addressing the cl...usion (ACDF) surgery., SlimFuse(TM) provides surg...
(Date:7/23/2008)...wire/ -- Tengion Inc., a clinical,stage biotechnol...ns and,neo-tissues, today announced that it has ap... and Commercial Planning. Mr. Stejbach brings more...nd managed care experience from,both Merck & Co., ...lcome Mark to the Tengion executive team as,we are...
(Date:7/23/2008).../ -- eFuture Information Technology,Inc. (Nasdaq: ...-end supply,chain management software and services...today announced that it has signed a contract to p...m 3.0 (,mySHOP 3.0, or ,the,system,) to Tiens ...stall,the system at Tiens Group,s six distribution...
(Date:7/23/2008)...mpany of Intent to Grant Patent, LONDON, July 23 ...), a,leading European RNAi focused biotechnology c...cover for its lead product, Atu027. The European,P...tends to grant a,European patent covering Silence,...l give Silence broad exclusivity not only for Atu0...
Breaking Biology Technology:Pioneer(R) Surgical Technology, Inc. Announces the Market Launch of the SlimFuse(TM) Anterior Cervical Plate System 2Tengion Appoints Mark Stejbach Vice President of Marketing & Commercial Planning 2eFuture Signs Contract to Provide Tiens Group with Retail Business Management System 2eFuture Signs Contract to Provide Tiens Group with Retail Business Management System 3eFuture Signs Contract to Provide Tiens Group with Retail Business Management System 4eFuture Signs Contract to Provide Tiens Group with Retail Business Management System 5Silence Obtains Important Patent Protection for Lead Internal Development Compound, Atu027 2Silence Obtains Important Patent Protection for Lead Internal Development Compound, Atu027 3
...ding to the hyperboleers, there is a B-movie style...between the New Economy and the Old Economy. In ro...rk Stock Exchange, thumping its chest and proclaim...e Old Economy rose up and threw the New Economy ou...nnocent bystanders in its fall. Of course, anyone ...
...lion provider of medical imaging, point-of-care sy...logy, announced today that it has received top rec...healthcare industry authority, KLAS Enterprises fo... Centricity(R) Pharmacy Information System, was re...rprises , a research and consulting firm specializ...
...saga of the consolidating pharmaceutical industry ...the news over the last two weeks, were talking abo... Sanofi-Synthelabo to take over French-German pha... a real major pharma player in France. If it happe...hird-largest pharmaceutical company after No. 1 P...
...sponse, it looks as if Small Tree Communications ... of former Cray and SGI employees to brave the mar...ver Grove Heights, Minn.-based business, with ties...touting its link aggregation mechanism as a breakt...omers who need enhanced networking for the Apple ...
Other Biology Technology:The Old Economy Vs. the New Economy 2The Old Economy Vs. the New Economy 3The Industry Impact of Two Big Pharmas Mating 2The Industry Impact of Two Big Pharmas Mating 3The Industry Impact of Two Big Pharmas Mating 4The Industry Impact of Two Big Pharmas Mating 5The Industry Impact of Two Big Pharmas Mating 6The Industry Impact of Two Big Pharmas Mating 7Former SGI/Cray Team Develops Products for G5 Platform 2Former SGI/Cray Team Develops Products for G5 Platform 3
(Date:7/22/2008)...s has identified a protein able to stimulate the p...ed in the recognition and the elimination of infec...Institute for Research in Immunology and Cancer (I... University of Oulu in Finland published in the l...p to combat age-related declines in immune respons...
(Date:7/22/2008)...iologist Matthew Fields spends his days studying a...ed. , Fields studies the physiology and behavior...ed virtually every square inch of the earth,s surf...s have global impact," Fields said. "They grow fas...in numbers." , Fields is particularly interested...
(Date:7/22/2008).... , ,, Plants undergo stress because of l...cess of light. A research team from the University...s that are triggered in plants to protect themselv...best suited to the environment during reforestatio...ghts, extreme temperatures, contamination, and so ...
(Date:7/22/2008)...fectively Treat Biofilms That Cause Cavities and G...sts to cure resin fillings and hydrogen peroxide (...treating bacteria in deep layers of biofilms that ...s from Hebrew University, Hadassah, Jerusalem, Isr... report their findings in the July 2008 issue of t...
Breaking Biology News(10 mins):Identification of protein able to stimulate production of T-cells 2MSU researcher uses grant to study little-known but largely useful microbes 2Measuring the stress of forested areas 2Measuring the stress of forested areas 3Tips from the Journals of the American Society for Microbiology 2Tips from the Journals of the American Society for Microbiology 3Repellents between dusk and bedtime make insecticide treated bednets more effective 6068 1Repellents between dusk and bedtime make insecticide treated bednets more effective 6068 2Research suggests mechanism for acne drugs link to depression 6066 1Research suggests mechanism for acne drugs link to depression 6066 2An Alzheimers vaccine 3F 1179 1An Alzheimers vaccine 3F 1179 2An Alzheimers vaccine 3F 1179 3Luxembourg joins EMBL 1177 1Luxembourg joins EMBL 1177 2
... motexafin gadolinium (brand name: Xcytrin, manufa...cer cells by disrupting key enzymes involved in ce...ers led by Joseph Hacia, Ph.D., assistant professo...k School of Medicine of the University of Southern...increases in the amount of zinc available inside t...
...oped to track enemy troop movements during the Vie...e elephant populations, according to new research ...nal of Applied Ecology. , Dr Jason Wood and collea...ons from the footfalls of elephants and other larg...sing a geophone buried near a path leading to a wa...
...rsity scientists in the Department of Biological S...-year-old evolutionary process in the human genome...sted in a latent state for long periods of time ma...chers say that these elements have played a crucia...ing hyperactive progeny copies, giving rise to the...
...atories has completed a two-year study of the pote...posure to depleted uranium (DU) during the 1991 Gu...rsal and Health Effects Using a Gulf War Case Stud...loys analytical capabilities used by Sandia,s Nati...th risks associated with uranium handling. , U.S....
Other Biology News:USC researchers determine mechanism of action of chemotherapy drug 2Vietnam war technology could aid elephant conservation 2Scientists track stealth DNA elements in primate evolution 2Scientists track stealth DNA elements in primate evolution 3Sandia completes depleted uranium study 2
Human IgG Fd. ELISA Specificity: (4) ,human IgG subclass 1 100% ,human IgG subclass 2 100% ,human IgG subclass 3 100% ,human IgG subclass 4 99% ,hu
Human VEGF R2 (KDR) MAb (Clone 89106)
Salmonella typhimurium Immunogen: Heat inactivated bacterial cells of Salmonella typhimurium B Storage: 4 C
TNT® T7 Quick Coupled Transcription/Translation System from Promega
Biology Products: