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Total RNA Isolation From 500 l Body Fluid

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6. Invert and drain the tube on clean absorbent paper and allow sample to air-dry for 15 min.

RNA Hydration
1. Add 100 l RNA hydration solution (100 l will give a concentration of 100 g/ml if the total yield is 10 g RNA).

2. Allow RNA to rehydrate for at least 30 min on ice. Alternatively, store purified RNA sample at -70 to -80C until use.

3. Before use, vortex sample vigorously for 5 sec and pulsespin. Pipet sample up and down several times to mix the sample further.



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