Navigation Links
Total RNA Isolation From 500 l Body Fluid


AquaPure RNA Isolation Kit
Catalog #732-6370


Method
Cell Lysis
1. Vigorously add 2.5 ml cell lysis solution to an Oak Ridge centrifuge tube (or other tube rated for high speed) containing 500 l body fluid (e.g., cerebrospinal fluid, plasma, saliva, serum, sputum, synovial fluid, urine, whole blood).

2. Vortex for 5 sec to mix thoroughly.

3. Heat sample to 65C for 5 min to complete lysis. Cool sample to room temperature. (This step is optional when testing for certain viruses such as hepatitis C.)

Protein-DNA Precipitation
1. Add 1 ml protein-DNA precipitation solution to the lysate.

2. Cap tube and invert gently 10 times and place tube into an ice bath for 10 min. 3. Centrifuge at 15,000 x g for 5 min. The precipitated proteins and DNA will form a tight pellet.

RNA Precipitation
1. Pour the supernatant containing the RNA (leaving behind the precipitated protein-DNA pellet) into a clean Oak Ridge centrifuge tube (or other tube rated for high speed) containing 3 ml 100% isopropanol (2-propanol). If RNA yields are expected to be low, add a glycogen solution as a carrier to the isopropanol (5 l of 20 mg/ml glycogen per 3 ml isopropanol).

2. Mix the sample by capping and inverting gently 50 times.

3. Centrifuge at 15,000 x g for 5 min; depending on yield, the RNA may or may not be visible as a small, translucent pellet.

4. Pour off supernatant and drain tube briefly on clean absorbent paper. Add 3 ml 70% ethanol, cap the tube, and invert it several times to wash the RNA pellet.

5. Centrifuge at 15,000 x g for 2 min. Carefully pour off the et
'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Total RNA Isolation From Body Fluid

(Date:1/14/2014)... Diego, CA (PRWEB) January 14, 2014 ... on the products of cells grown under simulated embryonic ... international license agreement with Suneva Medical, Inc. for physician-dispensed ... (CCM). , This agreement is an amendment ...
(Date:1/14/2014)... , Jan. 14, 2014 As pet owners drew up ... many of them wrote: "Take better care of my furry companion." ... designer clothes and top-brand carrying cases to take the little canine ... might also feel compelled to buy some pricey toys at the ...
(Date:1/14/2014)... 14, 2014 The largest international professional organization ... and therapeutic derivatives thereof has endorsed an educational ... about the challenges of adulterated herb and botanical ... The Society for Medicinal Plant and ...
(Date:1/14/2014)... 14, 2014 iLab Solutions, the leader in ... new Director of Product Strategy. In this role, Michelle will ... sub-teams to guide in the development of iLab products. Her ... maximum possible benefit to the scientific community by offering the ...
Breaking Biology Technology:Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... - Oncolytics Biotech Inc.,("Oncolytics") (TSX:ONC, NASDAQ:ONCY) announced ... pursue a pivotal (Phase II/III) randomized trial ... refractory,patients with head and neck cancers. The ... by the Company,s Board of Directors from ...
... team up in specialty biologics development, TORONTO ... and,Therapure BioPharma Inc. have agreed to partner in ... for cancer treatments, with a goal of,advancing the ... a,marketing partner to add to its portfolio of ...
... deCODE,genetics (Nasdaq: DCGN ) today announced today that ... Qualifications Panel to present a plan for,regaining compliance ... market value of listed securities of $50 million. ... Letter from The,Nasdaq Stock Market LLC indicating that ...
Cached Biology Technology:Oncolytics Biotech Inc. Announces Decision to Pursue Phase II/III Pivotal Clinical Trial 2Therapure Biopharma Inc. announces collaboration with BioVectra Inc. 2deCODE Requests Hearing With Nasdaq Following Receipt of Staff Determination Letter 2deCODE Requests Hearing With Nasdaq Following Receipt of Staff Determination Letter 3deCODE Requests Hearing With Nasdaq Following Receipt of Staff Determination Letter 4
(Date:4/17/2014)... RIVERSIDE, Calif. One day about eight years ago, ... of California, Riverside, and her father were on a field ... stumbled upon an orchid they had never seen before. , ... authority on orchids. The orchid turned out to be ... Silveras: Lophiaris silverarum . , "Lophiaris" is the genus ...
(Date:4/17/2014)... in France have hit on a novel method ... the correct and most effective treatment possible., Kidney ... western and developing world. If left untreated, apart ... renal failure and other complications. In many patients ... Clearly a more effective pathological approach to diagnosis ...
(Date:4/17/2014)... whereby the genetic information of DNA is used ... have numerous different functions in living organisms. Messenger ... gene expression, by relating the genetic information of ... proteins. , By examining the different types ... organism at a given time, researchers can determine ...
Breaking Biology News(10 mins):Orchid named after UC Riverside researcher 2Orchid named after UC Riverside researcher 3Rapid and accurate mRNA detection in plant tissues 2
... discovery by researchers at Oregon State University, the world,s multi-billion ... This industry, that produces metal castings used in everything from ... dates back thousands of years to before Greek and Roman ... but still continues to evolve. Some modern technologies use ...
... sites across the U.S. have contaminated groundwater that requires remediation, ... meaning restoration is unlikely to be achieved in the next ... new report from the National Research Council. The report ... sites ranges from $110 billion to $127 billion, but the ...
... becomes increasingly important to know in detail the dynamics of ... of a millimeter). What happens, for example, when we try ... molecules, such as DNA) through a nanopore if knots cause ... because now a new DNA sequencing method to electrochemically analyze ...
Cached Biology News:Sweet new approach discovered to help produce metal casting parts, reduce toxicity 2Report: Cleanup of some contaminated groundwater sites unlikely for decades 2Report: Cleanup of some contaminated groundwater sites unlikely for decades 3Pull with caution 2
Although each Beacon 2000 System includes one preinstalled sample chamber (size specified at time of order), optional chambers are available and easily retrofit by the user....
... Immunogen : Recombinant human ... 5mg/ml BSA, 0.15M NaCl, 0.05% ... of glycerol to 30% Quality ... on acid extracts of murine ...
cDNA Size Fractionation Columns offer an efficient and convenient method for size-fractioning double-stranded cDNA....
Self Closing Forceps...
Biology Products: