Navigation Links
Total RNA Isolation From 500 l Body Fluid


AquaPure RNA Isolation Kit
Catalog #732-6370


Method
Cell Lysis
1. Vigorously add 2.5 ml cell lysis solution to an Oak Ridge centrifuge tube (or other tube rated for high speed) containing 500 l body fluid (e.g., cerebrospinal fluid, plasma, saliva, serum, sputum, synovial fluid, urine, whole blood).

2. Vortex for 5 sec to mix thoroughly.

3. Heat sample to 65C for 5 min to complete lysis. Cool sample to room temperature. (This step is optional when testing for certain viruses such as hepatitis C.)

Protein-DNA Precipitation
1. Add 1 ml protein-DNA precipitation solution to the lysate.

2. Cap tube and invert gently 10 times and place tube into an ice bath for 10 min. 3. Centrifuge at 15,000 x g for 5 min. The precipitated proteins and DNA will form a tight pellet.

RNA Precipitation
1. Pour the supernatant containing the RNA (leaving behind the precipitated protein-DNA pellet) into a clean Oak Ridge centrifuge tube (or other tube rated for high speed) containing 3 ml 100% isopropanol (2-propanol). If RNA yields are expected to be low, add a glycogen solution as a carrier to the isopropanol (5 l of 20 mg/ml glycogen per 3 ml isopropanol).

2. Mix the sample by capping and inverting gently 50 times.

3. Centrifuge at 15,000 x g for 5 min; depending on yield, the RNA may or may not be visible as a small, translucent pellet.

4. Pour off supernatant and drain tube briefly on clean absorbent paper. Add 3 ml 70% ethanol, cap the tube, and invert it several times to wash the RNA pellet.

5. Centrifuge at 15,000 x g for 2 min. Carefully pour off the ethanol.

6. Invert and drain the tube on clean absorbent paper and allow sample to air-dry for 15 min.

RNA Hydration
1. Add 100 l RNA hydration solution (100 l will give a concentration of 100 g/ml if the total yield is 10 g RNA).

2. Allow RNA to rehydrate for at least 30 min on ice. Alternatively, store purified RNA sample at -70 to -80C until use.

3. Before use, vortex sample vigorously for 5 sec and pulsespin. Pipet sample up and down several times to mix the sample further.



back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Total RNA Isolation From Body Fluid

(Date:7/25/2014)... A new study by researchers from the University ... tiny nanosystems function, unlocking the potential to create ... study, which has been published in the prestigious ... a novel laser technique to examine in rich ... a small cluster containing an acetylene molecule and ...
(Date:7/25/2014)... 25, 2014 Lawrence Livermore scientists Charles ... Reuters list of " The World's Most Influential Scientific ... the 3,000 researchers who were identified by analyzing citation ... who published the highest-impact work (2002-2012 and 2012-2013). The ... combustion modeling . , "This recognition is a ...
(Date:7/24/2014)... July 24, 2014 -- Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) (the ... randomized, active control Phase 3 study of Zerenex (ferric ... for the treatment of hyperphosphatemia in patients with end-stage ... binding and iRon delivery with FErric CiTrate in EsrD) ... the American Society of Nephrology ( JASN ). ...
(Date:7/24/2014)...  ECC West Africa, LLC (ECC) signed a contract ... College Hospital (UCH) Ibadan Board of Management for the ... construct, and operate and maintain a new, state-of-the-art Cancer ... the first of the 4-phase project, ECC will conduct ... UCH Ibadan intends to finance and develop the Cancer ...
Breaking Biology Technology:Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 2Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 3Scientists Burst onto Influential List for Combustion Research 2Scientists Burst onto Influential List for Combustion Research 3Scientists Burst onto Influential List for Combustion Research 4Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2
... CAMBRIDGE, Mass. Chemists and engineers at Harvard University have ... enough to be used for sensitive probing of the interior ... the journal Science , is smaller than many viruses ... to take cellular measurements, which can be nearly as large ...
... as a 2010 American Chemical Society Fellowa distinguished group of ... or industry. Donna Nelson, professor in the OU Department ... ceremony at the ACS National Meeting in Boston on August ... second group of chemists to be honored for contributions to ...
... Faculty of Medicine scientists who proved it is possible to ... silicon chip or the brain on a microchip ... monitors brain cell activity at a resolution never achieved before. ... the new silicon chips are also simpler to use, which ...
Cached Biology Technology:New nanoscale transistors allow sensitive probing inside cells 2Neurochip technology developed by Canadian team 2
(Date:7/25/2014)... 2014 Biometrics industry expands and ... digital technology for online & commerce payments while seeking ... GOOG ), LifeLock Inc. (NYSE: ... Apple Inc. (NASDAQ: AAPL ) and Fortinet ... a biometric authentication company focused on the growing m-commerce ...
(Date:7/24/2014)... sobering examples of how wildlife loss leads to conflict ... by Wildlife Conservation Society (WCS) Health & Ecosystems: ... calls for an interdisciplinary approach to tackle global biodiversity ... about 15% of the world,s people and provides protein ... It should come as no surprise that today,s unprecedented ...
(Date:7/24/2014)... 2014 /PRNewswire-iReach/ -- The Geneva Healthcare Suite, an innovative ... cut emergency room wait times by an average of ... Center, according to a recent study published in the ... using the suite to access data from incoming patients, ... "Using Geneva,s technology platform we have been able ...
Breaking Biology News(10 mins):Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 2Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 3Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 4Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 5Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 6New study draws links between wildlife loss and social conflicts 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 3
... of specific neurons within the brain. It affects a person,s ... movement and loss of muscle co-ordination. New research published ... shows that the RyanR inhibitor Dantrolene is able to reduce ... model of HD. Progressive damage to medium spiny ...
... Lumbering around during the final weeks before delivery is tough ... compensate for the extreme physical changes that accompany the later ... find a distinct gait change such as a change ... stages of pregnancy. Intrigued by the ways that newborn dolphins ...
... 2011 Against the backdrop of the growing epidemic of ... of The Scripps Research Institute have made an important new ... in keeping a steady balance between our food intake and ... keys to fighting obesity and related disorders such as diabetes. ...
Cached Biology News:Pregnancy is a drag for bottlenose dolphins 2Scripps Research scientists uncover new role for gene in maintaining steady weight 2
Rabbit polyclonal to JAK1 (phospho Y1022 + Y1023) ( Abpromise for all tested applications). entrezGeneID: 3716 SwissProtID: P23458...
Perm/Wash Buffer 250 Tests...
PCR purification kit, 100 preps...
The BD PhosFlow Fix Buffer I can be used for the simultaneous fixation and permeabilization of cells prior to intracellular staining. Applications: Intracellular Staining (Flow) Storage Temperature:...
Biology Products: