Navigation Links
Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus

n tube on clean absorbent paper. Add 600 l 70% ethanol and invert the capped tube several times to wash the RNA pellet.

5. Centrifuge at 13,00016,000 x g for 1 min. Carefully pour off the ethanol.

6. Invert and drain the tube on clean absorbent paper and allow to air-dry 1015 min.

RNA Hydration
1. Add 20 l RNA hydration solution (20 l will give a concentration of 50 g/ml if the total yield is 1 g RNA).

2. Allow RNA to rehydrate on ice for at least 30 min. Store purified RNA sample at 70 to 80C until use.

3. Before use, vortex sample vigorously for 5 sec and pulsespin. Pipet sample up and down several times to ensure adequate mixing.



back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
(Date:2/26/2015)... , Feb. 26, 2015 DuPont Executive ... priorities driven by key research advancements and product launches ... today,s Bank of America Merrill Lynch 2015 Global Agriculture ... challenge of building grain supplies the last two years, ... at the pace of the last decade, when demand ...
(Date:2/26/2015)... , Feb. 26, 2015 The healthcare landscape ... ever-changing arena, Frost & Sullivan will host its 20 ... Executive MindXchange , March 8 to 10, at the Hilton ... Calif. This event is a platform ... leaders to discuss industry disruptions and how to succeed in ...
(Date:2/26/2015)... 26, 2015 Innovation is more ... from unexpected parts of the world and transforming ... agriculture to manufacturing to health care, disruption is ... realities and socio-political trends shake the foundations of ... ingredient for CEOs to survive the next wave ...
(Date:2/26/2015)... February 26, 2015 Regis Technologies ... development of oncology drug substances. , Regis Technologies is ... their new Potent Compound Suite (PCS) for 2015. , ... square foot, cGMP facility in Morton Grove, Illinois. The ... of potent compounds up to about one kilogram per ...
Breaking Biology Technology:DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 2DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 3DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 4DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 5Embrace the Future of Medical Technologies with Frost & Sullivan 2Embrace the Future of Medical Technologies with Frost & Sullivan 3One Million Acts of Innovation Canada Creates Partnership with The Economist Events’ Innovation Forum 2Regis Extends cGMP Services to Oncology Market 2
... Brian J. Shiell and Wojtek P. Michalski, CSIRO Division of Animal Health,Australian ... , Introduction ... of proteins can be performed , on ... the most commonly used method of recovering proteins from gels, can be , ...
... M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries ... , Introduction ... sensitive method for the detection of nucleotide , ... markers. Denaturing gradient gel electrophoresis (DGGE) detects sequence ...
... David A. Crispin,* Ru Chen,* Michael B. Kimmey,** Teresa A. Brentnall** , ... , **Medicine, Division of Gastroenterology, University of Washington, Seattle, , ... , Introduction , ... , and its frequency is rising. At the time of ...
Cached Biology Technology:Fractionation of Bovicola ovis Homogenates Using the Mini Whole Gel Eluter 2Fractionation of Bovicola ovis Homogenates Using the Mini Whole Gel Eluter 3Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 2Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 3Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 4Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 5Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 6Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 7Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System 8Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE 2Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE 3
(Date:2/24/2015)... NEW YORK , Feb. 24, 2015 This ... Thousands. The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... America . Annual estimates and forecasts are provided for ... is provided for these markets. Market data and analytics are ...
(Date:2/19/2015)... Feb .19, 2015 Research and Markets ... of the "Military Electro-Optical / Infrared Systems ... by Platform - Forecast to 2020" report ... electro-optical/infrared systems market is expected to reach $16.35 ... 7.71%. This report segments the military ...
(Date:2/12/2015)... OXFORD, Conn. , Feb. 12, 2015 ... ("NXT-ID" or the "Company"), a biometric authentication company focused ... it has filed provisional patent 62/113114 for ... MULTIPLE DEVICES. The patent further establishes NXT-ID,s ... to its intellectual property portfolio the ability for multiple ...
Breaking Biology News(10 mins):World Iris Biometrics Industry 2World Iris Biometrics Industry 3World Iris Biometrics Industry 4World Iris Biometrics Industry 5World Iris Biometrics Industry 6World Iris Biometrics Industry 7World Iris Biometrics Industry 8World Iris Biometrics Industry 9World Iris Biometrics Industry 10World Iris Biometrics Industry 11Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 3NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 2NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 3
... 17, 2009) Tufts University researchers have identified a gene-diet ... replicated their findings in three independent studies. Men and ... index (BMI) scores and a higher incidence of obesity, ... saturated fat. These associations were seen in the apolipoprotein ...
... , REDWOOD CITY, Calif., Nov. 17 DigitalPersona, ... solutions, today announced that its customer, Picis, has ... and privacy protection for its Picis ED PulseCheck® ... U.are.U® Fingerprint Readers and One Touch® for Windows ...
... WASHINGTON DC, NOVEMBER 16, 2009 Experts in soil ... will present a Congressional educational briefing, "The Essence of Earth ... in 2168 Rayburn House Office Building (Gold Room). ... concept of "terroir" -- how the balance of vineyard management ...
Cached Biology News:Nutrigenomics researchers replicate gene interaction with saturated fat 2Nutrigenomics researchers replicate gene interaction with saturated fat 3Picis ED PulseCheck Increases Security and Privacy Protection in Nearly 150 Hospitals Through the Use of DigitalPersona Biometrics 2Picis ED PulseCheck Increases Security and Privacy Protection in Nearly 150 Hospitals Through the Use of DigitalPersona Biometrics 3Soil experts to discuss terroir, the link between Earth, wine on Nov. 19 in DC 2
...
... area that will help protect your PCR ... the work area prior to use, reducing ... amplified. Containment features reduce the chance of ... windows for a clear view, an acid-resistant ...
The large Dodeca stainer shaking rack holds the staining trays in the large Dodeca stainer....
... is the most advanced thermal cycler ever ... performance, the Dyad also boasts graphical programming ... navigation via touch pad or mouse. ... economically expanded to a four-bay system with ...
Biology Products: