( f glycosylated and nonglycosylatedpr...)Tools for Proteomic Science II

f glycosylated and nonglycosylated proteins in a single lane is also possible.

Labeling and detection

With the nonradioactive coupling reagents and the Protein Labeling Kits, Roche Applied Science provides a simple and rapid method for the labeling of proteins with alkaline phoshatase, biotin, digoxigenin, fluorescein, or peroxidase. Streptavidin and antibody conjugates recognize biotin or digoxigenin and fluorescein, and allow the convenient detection of labeled proteins.

Epitope tagging

Epitope tagging eliminates the laborious and time-consuming task of producing a new antibody every time a protein is studied. Our anti-Tag antibodies simplify the detection, characterization, and purification of proteins. They are also available in conjugated forms, and recognize commonly used epitope sequences, such as the influenza hemagglutinin protein (HA), human c-myc protein, six histidine residues (His6), and others. The epitope sequences can be easily inserted at the desired position within the protein amino-acid sequence using recombinant DNA techniques. In particular, our anti-HA antibody (clone 3F10) represents a powerful antibody for detection and purification due to its high affinity.

Western blotting

Detect your protein by colorimetric or chemiluminescence methods: The detection of proteins using chemiluminescence has become the method of choice, with peroxidase and luminol reagents being the most popular chemiluminescence system for protein detection. You can save sample material by using our sensitive Lumi-LightPLUS Western blotting substrates, kits, and polyvinylidene difluoride (PVDF) membranes. The Lumi-LightPLUS substrate enables multiple exposures due to the high stability of the s
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