( ...)Tools for Proteomic Science II

Now that we know complete genomes, we want to understand the functions of proteomes, the sum of all proteins encoded by a genome at a specific stage. Assembling the protein puzzle presents an even more challenging task than the sequencing of the genome. The reason for this is the complexity of protein structure and function, and the sensitivity of these proteins to environmental conditions. The combination of accuracy, efficiency, and a gentle touch are critical for obtaining optimal results from your protein research. Achieving the best outcome requires the best input and that is where Roche Applied Science comes in. We at Roche Applied Science utilize our 50 years of experience in the development of prime tools to meet your evolving research needs.

During the last few decades, Roche Applied Science has developed many techniques and reagents for protein research based on our competence in purification and stabilization, labeling and detection, protein expression and cell biology. Roches proteomics group is using these tools in diagnostic and pharmaceutical research, and for the development of high-tech drugs.

In this second Biochemica article, we continue with a summary of our most important products for proteomic science

Protein Characterization

Glycan analysis

Obtain detailed information on the amounts of carbohydrate and their structures. Using our glycoprotein analysis system you can determine whether or not your protein is glycosylated, whether the sugar moiety is N- or O-linked, as well as gather additional data regarding carbohydrate structures. Quantification of the sugar moiety and simultaneous staining of glycosylated and nonglycosylated proteins in a single lane is also possible.

Labeling and detection

With the nonradioactive coupling reagents and the Protein Labeling Kits, Roche Applied Science provides a simple and rapid method for the labeling of proteins with alkaline phoshatase, biotin, digoxigenin, fluorescein, or peroxidase. Streptavidin and antibody conjugates recognize biotin or digoxigenin and fluorescein, and allow the convenient detection of labeled proteins.

Epitope tagging

Epitope tagging eliminates the laborious and time-consuming task of producing a new antibody every time a protein is studied. Our anti-Tag antibodies simplify the detection, characterization, and purification of proteins. They are also available in conjugated forms, and recognize commonly used epitope sequences, such as the influenza hemagglutinin protein (HA), human c-myc protein, six histidine residues (His6), and others. The epitope sequences can be easily inserted at the desired position within the protein amino-acid sequence using recombinant DNA techniques. In particular, our anti-HA antibody (clone 3F10) represents a powerful antibody for detection and purification due to its high affinity.

Western blotting

Detect your protein by colorimetric or chemiluminescence methods: The detection of proteins using chemiluminescence has become the method of choice, with peroxidase and luminol reagents being the most popular chemiluminescence system for protein detection. You can save sample material by using our sensitive Lumi-LightPLUS Western blotting substrates, kits, and polyvinylidene difluoride (PVDF) membranes. The Lumi-LightPLUS substrate enables multiple exposures due to the high stability of the signal after substrate addition (stable for more than 9 hours).

ELISA

An enzyme-linked immunosorbent assay (ELISA) for biomedical research must be carefully developed and excellently manufactured. ELISAs from Roche Applied Science are designed to be quick and accurate. One-step immunoassays save time and result in less error. Special additives eliminate interference from human anti-mouse antibodies (HAMA). We offer ELISAs for different fields in drug and disease research, such as cytokine and growth-hormone research, oncology research, and others. Moreover, streptavidin-coated microplates (Strepta- Well) with high binding capacity for chemiluminescence, colorimetric, or fluorescence detection are available.

Pathway Analysis

Reporter gene assays

Roche Applied Science offers a broad line of sensitive kits and reagent sets for reporter gene assays on transfected cells. These nonradioactive products measure promotor activity with a sensitivity equal to or greater than that of radioisotopic assays, providing a safe alternative without sacrificing sensitive results.

Apoptosis and cell proliferation

Apoptosis- and cell proliferation-related genes and proteins have become quite relevant putative drug or diagnostic targets since many studies have demonstrated that deregulation of cell death is associated with a variety of diseases. These pathological conditions are accompanied either by cell accumulation (e.g., cancer) or cell loss (e.g., Alzheimers disease). After the identification of a putative target in the proteomics workflow, its function and relevance, as well as the efficacy of specific drugs must be further characterized. For the study of apoptosis- and cell proliferation-related targets, Roche Applied Science provides many different research tools to fulfill the demands of such screening studies.

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