Navigation Links
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

s: The Northern blot containing total RNA from CHO cells was cut into duplicate sections, and each was prehybridized in 3 ml of Stratagenes QuikHyb hybridization solution for 15 minutes in Stratagenes PersonalHyb hybridization oven, according to the QuikHyb manuals instructions. Each blot was hybridized with 3 x 106 cpm of one of the probes (106 cpm/ml) for 1 hour, also according to the manual. The membranes were washed twice for 10 minutes in 2X SSC/0.1% SDS at room temperature and twice for 15 minutes with 0.1X SSC/0.1% SDS at 60C. The membranes were enveloped in plastic wrap and exposed to Kodak XAR-5 film at -80C (with an intensifying screen) for varying amounts of time. An overnight exposure detected the low to moderate-abundance MSH2 transcript from as little as 200 to 500 ng of total RNA. After a 4-day exposure, the expected 3.1-kb band was observed from as little as 100 ng of total RNA, with a minimum of background (Figure 2).

Fig.2

REFERENCES

  1. Palombo, F., et al. (1994) Nature 367: 417.

  2. Varlet, I., et al. (1994) Nuc. Acids Res. 22: 57235728.

  3. Dolter, K. and Braman, J. (1999) Strategies 12: 3.

  4. Blakely, M. (1988) Strategies 2: 78.

  5. Mackman, C.H., Kubitz, M., and Willbanks, A. (1990) Strategies 3: 2627.

  6. Dycaico, M. (1997) Strategies 10: 4849.

  7. Braman, J. and Basehore, S. (1997) Strategies 10: 8486.

  8. Felts, K., et al. (1992) Strategies 5: 3738.

  9. Jerpseth, B
    '"/>



Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Tools for Proteomic Science II
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. New Tools for Small RNA Analysis
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
8. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
9. Detecting miRNA & siRNA
10. Detecting Attomole Amounts of Small RNA
11. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tools for Detecting MSH2 Expression Chinese Hamster Ovary Cells

(Date:9/2/2015)... 2015 Veracyte, Inc. (NASDAQ: VCYT ) ... and chief executive officer, will present at the Morgan ... at 10:30 a.m. ET in New York, ... webcast and subsequent replay may be accessed by visiting Veracyte,s website ... shortly after conclusion of the presentation and archived on ...
(Date:9/2/2015)... 2015 Biovista Inc. freut ... von 2015 bekanntgeben zu können. Hierbei handelt es ... Biovista unterstützt mit seiner Möglichkeit der ... anderer Krankheiten als denen, für die ein Medikament ... sein Team bei der Wylder Nation Foundation bei ...
(Date:9/2/2015)... ... 02, 2015 , ... Temarry Recycling's new $100,000 waste to ... process and continue to contribute to the sustainability of our natural resources. , ... process has been operating at their Mexico facility, Recicladora Temarry, for the last ...
(Date:9/1/2015)... , September 2, 2015 ... auf die Forschung und Entwicklung neuartiger Therapien ... chronisch-entzündliche Darmerkrankungen und Reizdarm konzentriert, erhält vom ... dass sein neues Medikament patentiert wird. Das ... Allergien bei Kindern verhindert und Entzündungen vorbeugt.   ...
Breaking Biology Technology:Veracyte to Present at Morgan Stanley Global Healthcare Conference 2Biovista sponsert die Forschung von Wylder Nation Foundation zum Thema Mangel an saurer Sphingomyelinase 2Temarry Recycling’s New $100,000 Waste to Energy Renovation 2Das Patent von Swecure zur Verhinderung von Allergien bei Kindern wird in den USA zugelassen 2
... system for boats and recreational vehicles. Think about a ... hikers, campers and sportsmen. Consider a company that produces ... for cows, which, in turn, is better for farmers. ... welcome to the third annual Governor's Business Plan Contest. ...
... have stepped forward in an attempt to prevent a young ... state, but outside investors are still in the picture. , ... will meet with four prospective Wisconsin investors on May 18 ... in Green Bay. Those meetings will precede talks with investors ...
... The first embryonic stem cells were isolated in mice in ... derive stem cells from human embryos. That kicked into full ... a look at key moments in the controversy so far: ... of Wisconsin isolate the first embryonic stem cells in ...
Cached Biology Technology:Business plan contest finalists reflect state's economic character 2Business plan contest finalists reflect state's economic character 3Wisconsin investors will make their pitch to IPIC 2A brief timeline of the stem cell debate 2A brief timeline of the stem cell debate 3A brief timeline of the stem cell debate 4A brief timeline of the stem cell debate 5A brief timeline of the stem cell debate 6
(Date:8/11/2015)... Today, ZUK announced its Android smartphone Z1, featuring FPC,s touch ... 2015 that relate to sales of FPC1155 for this smartphone model ... for 2015. Jörgen Lantto, CEO of FPC, comments: " ... manufacturer in China and we are proud ... for Z1 , its first ...
(Date:8/10/2015)... The latest 364 page report from ... the global border security market . Visiongain ... 2015. Now: Border security is hard to achieve ... business critical issue you need to know about - and ... analysis of how this will impact your company and the ...
(Date:8/6/2015)... TELTOW, Germany , August 6, 2015 ... SensoMotoric Instruments (SMI) shows the world,s first ... based on Epson,s Moverio BT-200 see-through head mounted ... this new solution, unprecedented quality and efficiency is brought ... context-sensitive displays. For the first time, professionals and researchers ...
Breaking Biology News(10 mins):FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3
... Conservation Biology annual meetings, in Brasília, Brazil, the Woods ... da Amazonia (IPAM) will hold an international symposium on ... the Amazon Basin. , This region has entered a ... industries driving deforestation (cattle ranching and soybean farming) are ...
... available online) of Nature Genetics establishes a model ... cells in the human body determine their own ... associate professor of pediatrics and of medical and ... Medicine, investigated the interaction of proteins responsible for ...
... you being in a car wreck or being bitten by a ... a free trip to the beach. It's really no contest, says ... wreck are far greater than being a shark's lunch, says John ... sharks for years. , Worldwide, about one million people a year ...
Cached Biology News:Determining The Fate Of Cells In The Human Body 2Shark attack worries? Driving to the beach is more deadly 2