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Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

modest expression level of this RNA make it a good candidate for evaluating products for Northern blot analysis.

Fig.1

Stratagenes products for optimized Northern blot analysis (Figure 1) demonstrate substantial benefits: The StrataPrep total RNA miniprep kit allows pure total RNA to be isolated quickly and easily with high yields. Gels prepared with the Joule Box minigel electrophoresis system run fast and generate consistent results. Capillary transfer of RNA is efficient with the Duralon-UV membrane, and the Stratalinker enables rapid and simple immobilization of RNA on the membrane.

The cDNA synthesized with the ProSTAR first strand RT-PCR kit is suitable for many uses (including PCR). Stratagenes PCR primers, including primers for MSH2 transcripts, are optimized for RT-PCR. An MSH2 fragment is readily generated from cDNA during PCR using these primers. With the StrataPrep PCR purification or DNA gel extraction kit, DNA fragments purified after PCR are ready to be used in primer labeling reactions.

The Prime-It II random primer labeling kit is used to label DNA fragments to high specific activity in just a few minutes. Unincorporated nucleotides can be readily and swiftly removed from these probes using NucTrap probe purification columns. When hybridization of these probes is performed in QuikHyb solution, the hybridization occurs rapidly and with minimal background. The PersonalHyb hybridization oven is used with various sizes of tubes, avoiding the tedious manipulations required when using sealed bags for hybridization reactions. Additionally, the accurate and precise temperature control of this oven assures consistent results.

Conclusions

Use Stratagenes products for Northern blot analysis to achieve outstanding results with substantial time sav
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