Navigation Links
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells


Integrated system simplifies and improves Northern blot analysis

Karen Dolter Jeff Braman
Stratagene

Streamline RNA analysis with a whole host of products from Stratagene. First, the StrataPrep total RNA miniprep kit is used to isolate ample yields of high-quality RNA from tissues and cells, then the RNA is ready to use in a number of applications, including Northern blot analysis. This procedure features the following products: the Joule Box minigel electrophoresis system, the Stratalinker UV crosslinker,* the prostar first strand RT-PCR kit, Stratagenes primer sets for RT-PCR, the StrataPrep PCR purification kit or the StrataPrep DNA gel extraction kit, the Prime-It II random primer labeling kit, NucTrap probe purification columns,** QuikHyb hybridization solution, and the PersonalHyb hybridization oven. When used together for Northern blot analysis, this excellent integrated system permits high sensitivity and reproducible results.

Northern blot analysis of gene transcription is dependent on such factors as isolating intact, high-quality RNA, probe-labeling to high specific activity, removing unincorporated radiolabeled nucleotides, and hybridizing with conditions that maximize the signal-to-noise ratio. Products developed at Stratagene eliminate many variables that lead to poor quality Northern blot results. Consequently, we used these products for a Northern blot analysis of MSH2 gene transcription in a hamster cell model system.

MSH2 is important for DNA mismatch repair, and mutations in this gene are responsible for many cases of hereditary nonpolyposis colorectal cancer.1 The MSH2 gene encodes a 3.1-kb mRNA that is expressed at low to moderate levels.2 The larger-than-average size and
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Tools for Proteomic Science II
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. New Tools for Small RNA Analysis
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
8. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
9. Detecting miRNA & siRNA
10. Detecting Attomole Amounts of Small RNA
11. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tools for Detecting MSH2 Expression Chinese Hamster Ovary Cells

(Date:8/21/2014)... Rockville, MD (PRWEB) August 21, 2014 ... is making progress in its development of JRC-LAMP-vax, an ... red cedar pollen. Phase IC studies began this month ... solution to hay fever. , The Japanese red cedar ... million people in Japan. Mountain Cedar pollen wreaks similar ...
(Date:8/21/2014)... 21, 2014 His Majesty Willem-Alexander, King ... The Court of His Majesty Willem-Alexander, King of the ... Grand Opening of POET-DSM Advanced Biofuels’ Project LIBERTY cellulosic ... , The King will take part in the Grand ... for 11 a.m.-12:20 p.m. and tour the plant. POET-DSM ...
(Date:8/21/2014)... Aug. 21, 2014 OTC Markets ... Inc., a biotechnology company, on its approval to ... traded on OTCQX®, the best marketplace for established ... Group. Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO ... successful execution of its growth strategy and achievement ...
(Date:8/21/2014)... St. Louis, MO (PRWEB) August 21, 2014 ... premier, full service biopharmaceutical contract development and manufacturing ... selected by Progenics Pharmaceuticals, Inc., an oncology company ... targeting and treating cancer, to manufacture the anti-prostate ... Progenics’ PSMA ADC product candidate. Under the ...
Breaking Biology Technology:Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 2Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 3Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 4His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 3
... , , , AUSTIN, Texas, Jan. ... announced its predictions for key trends and merger and acquisition ... the oil and gas sector for 2010. Bender Consulting combines ... transformation initiatives for High Tech, Energy and Bio Tech clients. ...
... , MADEIRA BEACH, Fla. , ... Mr. Diabetes®, Executive Director of Defeat Diabetes Foundation and diabetes ... ProtoKinetix (PKTX) Advisory Board.  ProtoKinetix, Inc. is a biotechnology company ... glycoproteins (AAGP™) for medicine and the biotechnology and cosmetic industries. ...
... Million EUR 2008 08/07 2009 ... MEUR %Sales Var.% MEUR %Sales Var.%, Southern Europe (1) ... countries (2) 11.3 23 17 13.1 23 ... 1.5 3 (25), SLIT ...
Cached Biology Technology:Bender Consulting Predicts Key Trends and Merger and Acquisition Activity for the Oil & Gas Sector in 2010 2Bender Consulting Predicts Key Trends and Merger and Acquisition Activity for the Oil & Gas Sector in 2010 3Andrew P. Mandell - Mr. Diabetes(R) to Sit On ProtoKinetix Advisory Board 2STALLERGENES : Sales Guidance Exceeded : EUR193 Million, up 13% 2STALLERGENES : Sales Guidance Exceeded : EUR193 Million, up 13% 3
(Date:8/21/2014)... the Fram Strait is a key region in the global ... Greenland and Svalbard warm Atlantic water flows to the north ... Arctic water masses and sea ice push their way out ... of the Atlantic water cools here on its way to ... the water caused in this manner drives the global band ...
(Date:8/21/2014)... of researchers at Louisiana Tech University has ... consumer-grade 3D printers and materials to fabricate ... and chemotherapeutic compounds for targeted drug delivery. ... and research faculty from Louisiana Tech,s biomedical ... create filament extruders that can make medical-quality ...
(Date:8/20/2014)... of 158 pregnant teenagers in Rochester, NY, nearly half ... of ice, cornstarch, vacuum dust, baby powder and soap, ... , Moreover, such teens had significantly lower iron levels ... substances. , Pregnant teens, regardless of pica, are at ... iron deficiency and anemia. Low iron in pregnant teens ...
Breaking Biology News(10 mins):Arctic sea ice influenced force of the Gulf Stream 2Arctic sea ice influenced force of the Gulf Stream 3Louisiana Tech University researchers use 3D printers to create custom medical implants 2Louisiana Tech University researchers use 3D printers to create custom medical implants 3Pica in pregnant teens linked to low iron 2
... of what makes some people stand head and shoulders above ... the first common version of a gene influencing height has ... together can make a height difference of up to 6cm. ... online today in the journal Nature Genetics, mean that scientists ...
... Those spindly plants that desperately try to reach for a ... from the same affliction: Shade avoidance syndrome or SAS. Now, ... by researchers at the Salk Institute for Biological Studies. ... on a natural chemical factory for the synthesis of the ...
... at the Burnham Institute for Medical Research (Burnham) may ... therapy by showing the link between a certain protein ... feed breast tumors. Like mortar between bricks in a ... together and collectively form tissues. Cancer cells that loosen ...
Cached Biology News:A tall story: New research adds to growing body of knowledge of genetics of height 2A place in the sun 2A place in the sun 3T-cadherin affects blood vessel growth in breast cancer, hormone from fat cells may play a role 2T-cadherin affects blood vessel growth in breast cancer, hormone from fat cells may play a role 3